Evaluation of Antimicrobial and Antibiofilm Activities of Lactic Acid Bacteria (LAB) Against Citrobacter spp. Isolates

Year 2023, Volume: 23 Issue: 1, 27 - 36, 01.03.2023

Safiye Elif Korcan , Sevim Feyza Erdoğmuş , Mine Erik , Arzu Ünal , Beytullah Kenar

https://doi.org/10.35414/akufemubid.1149039

Abstract

The aim of this research was to evaluate the antimicrobial and antibiofilm effects of LABs against Citrobacter isolates. In this study, Citrobacter isolates (C1, C2, C3) which were identified as Citrobacter braakii with the BD PhoenixTM automation system. Biofilm formation investigated by Congo red agar method and microtiter plate method. Acording to antibiogram test results, all isolates was resistance to ampicillin, amoxicillin-clavulanate. Antimicrobial activity test results revealed that extracts of LABs (Lactococcus lactis (L1), Lactobacillus fermentum (L2), Enterococcus faecalis (L3), Lactobacillus casei (L4), Lactobacillus plantarum (L5), Enterococcus faecium (L6), Lactobacillus curvatus (L7), Enterococcus durans (L8) Lactococcus garviae (L9), Enterococcus faecalis (L10)) extracts have an antimicrobial effect on Citrobacter braakii. The highest antimicrobial effect determined on C2 isolate and the lowest effect determined on C3. According to antibiofilm test results, it was observed that high doses of L1, L2, L4, L6, L7, L8 extracts inhibited biofilm formation in all Citrobacter isolates.

Keywords

Antibiotic resistance, Biofilm, Citrobacter spp., Lactic Acid Bacteria

Laktik Asit Bakterilerinin (LAB) Citrobacter spp. İzolatlarına Karşı Antimikrobiyal ve Antibiyofilm Aktivitelerinin Değerlendirilmesi

Abstract

Bu çalışmanın amacı; Citrobacter izolatlarına karşı laktik asit bakterilerinin antimikrobiyal ve antibiyofilm aktivitelerini belirlemektir. Bu çalışmada kullanılan Citrobacter izolatları (C1, C2, C3) BD PhoenixTM otomasyon sistemi ile Citrobacter braakii olarak tanımlanmıştır. Biyofilm oluşumu Kongo kırmızılı agar ve mikrotitrasyon plak metodu kullanılarak incelenmiştir. Antibiyogram test sonuçlarına göre, tüm izolatlar amfisilin ve amoksilin-klavulanata karşı dirençli bulunmuştur. Antimikrobiyal aktivite test sonuçları laktik asit bakterilerinden elde edilen ekstraktların (Lactococcus lactis (L1), Lactobacillus fermentum (L2), Enterococcus faecalis (L3), Lactobacillus casei (L4), Lactobacillus plantarum (L5), Enterococcus faecium (L6), Lactobacillus curvatus (L7), Enterococcus durans (L8) Lactococcus garviae (L9), Enterococcus faecalis (L10)) Citrobacter braakii üzerinde antimikrobiyal ve antibiyofilm etkinliğinin olduğu saptanmıştır. En yüksek antimikrobiyal etki C2 izolatı üzerinde ve en düşük etki C3 izolatı üzerinde belirlenmiştir. Antibiyofilm test sonuçlarına göre L1, L2, L4, L6, L7, L8 ekstraktlarının en yüksek dozlarının tüm Citrobacter izolatlarında biyofilm oluşumunu engellendiği görülmüştür.

Keywords

Antibiyobiyotik direnci, Biyofilm, Citrobacter spp., Laktik Asit Bakterileri

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