Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves

Year 2023, Volume: 23 Issue: 2, 292 - 299, 03.05.2023

Munise Yurtsever Maide Şeker , Şenay Vural Korkut

https://doi.org/10.35414/akufemubid.1205241

Abstract

One of the most prevalent environmental stresses that affects plants physically and physiologically is
the low temperature. Since low temperature adversely affects plant productivity Researchers
investigated the molecular processes that regulate the effects of cold stress on plants and discovered
many cold responsive genes as well as the control mechanisms that regulate them. CBF/DREB (C repeat
binding factor/dehydration response element binding factor) transcription factors induces the
expression of corresponding genes in plants in response to abiotic stress conditions such as cold,
drought and salinity. These transcription factors contribute to plant resistance to stress by activating
genes in various pathways. Genes encoding CBF transcription factors were first discovered in
Arabidopsis thaliana (L.). Later, Gene homologs were isolated and cloned from a variety of plants. They
belong to the AP2/EREBP protein family. In the present study, the full-length cDNA encoding homologue
of the transcription factor CBF1 was cloned from Olea europaea L. cv. Gemlik leaves. A 748 bp long
cDNA encodes a hypothetical protein of 224 amino acids. BLAST analysis revealed that the CBF1
sequences of Gemlik cultivar and wild olive were nearly identical. A phylogenetic tree was built using
Olea europea L. cv. Gemlik CBF1 sequence.

Keywords

CBF/DREB, Olea europaea L, Gene Cloning, DNA Sequencing, Phylogenetic analysis

Supporting Institution

TÜBİTAK

Project Number

1919B011703461

Thanks

This work was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK), project no 1919B011703461.

Olea europaea L. yaprağından tam uzunlukta CBF1 transkripsiyon faktörünü kodlayan gen homoloğunun klonlanması

Abstract

Bitkileri fiziksel ve fizyolojik olarak etkileyen en yaygın çevresel streslerden biri düşük sıcaklıktır. Düşük
sıcaklık bitki verimliliğini olumsuz etkilediğinden, araştırmacılar soğuk stresinin bitkiler üzerindeki
etkilerini düzenleyen moleküler süreçleri araştırmışlar ve soğuğa duyarlı genlerle birlikte bunların
kontrol mekanizmalarını da ortaya çıkarmışlardır. CBF/DREB (C tekrar bağlama faktörü/dehidrasyon
yanıt elemanı bağlama faktörü) transkripsiyon faktörleri, bitkilerde soğuk, kuraklık ve tuzluluk gibi
abiyotik stres koşullarına yanıt olarak ifade edilen genlerin ekspresyonunu indüklemektedir. Bu
transkripsiyon faktörleri, çeşitli yolaklardaki genleri aktive ederek strese karşı bitki direncine katkıda
bulunurlar. CBF transkripsiyon faktörlerini kodlayan genler ilk olarak Arabidopsis thaliana'da (L.)
keşfedildi. Daha sonra çeşitli bitkilerden gen homologları klonlandı. CBF/DREB transkripsiyon faktörleri
AP2/EREBP protein ailesi içinde yer alırlar. Bu çalışmada, transkripsiyon faktörü CBF1'in homologunu
kodlayan tam uzunluktaki cDNA, Olea europaea L. cv. Gemlik yapraklarından klonlanmıştır. 748 bp
uzunluğunda cDNA, 224 amino asitlik varsayımsal bir proteini kodlar. BLAST analizi, Gemlik çeşidinin ve
yabani zeytinin CBF1 dizilerinin neredeyse aynı olduğunu ortaya çıkardı. Olea europea L. cv Gemlik CBF1
dizisi kullanılarak bir filogenetik ağaç oluşturuldu.

Keywords

CBF/DREB, Olea europaea L, Gen Klonlama, DNA Dizileme, Filogenetik Analiz

Project Number

1919B011703461

References

• Agarwal PK, P Agarwal, MK Reddy, and SK Sopory, 2006. Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Reports, 25, 1263-1274.
• Baker SS, KS Wilhelm, and MF Thomashow, 1994. The 5′-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant Molecular Biology, 24, 701–713.
• Boyer JS, 1982. Plant productivity and environment. Science, 218(4571), 443–448.
• Caffagni A, N Pecchioni, E Francia, D Pagani, and J Milc, 2014. Candidate gene expression profiling in two contrasting tomato cultivars under chilling stress. Biologia Plantarum, 58, 283–295.
• Chen JQ, XP Meng, Y Zhang, M Xia, and XP Wang, 2008. Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnology Letters, 30, 2191–2198.
• Cultrera NGM, V Sarri, L Lucentini, M Ceccarelli, F Alagna, R Mariotti, S Mousavi, CG Ruiz, and L Baldoni, 2019. High levels of variation within gene sequences of Olea europaea L. Frontiers in Plant Science, 9, 1–17.
• Gilmour SJ, DG Zarka, EJ Stockinger, MP Salazar, JM Houghton, and MF Thomashow, 1998. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced CORgene expression. The Plant Journal, 16, 433–442.
• Guo HM, ZC Li, H Zhang, YZ Xin, and HM Cheng, 2011. Cloning of Cotton CBF Gene for Cold Tolerance and Its Expression in Transgenic Tobacco. Acta Agronomica Sinica, 37, 286–293.
• Huang B, LG Jin, and JY Liu, 2007. Molecular cloning and functional characterization of a DREB1/CBF-like gene (GhDREB1L) from cotton. Science in China, Series C: Life Sciences, 50, 7–14.
• Inoue H, H Nojima, and H Okayama, 1990. High efficiency transformation of Escherichia coli with plasmids. Gene, 96, 23–28.
• Jaglo-Ottosen KR, SJ Gilmour, DG Zarka, O Schabenberger, and MF Thomashow, 1998. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science, 280, 104–106.
• Liang N, L Yu, C Liu, Z Wang, X Zhao, and Y Zhan, 2019. Molecular cloning and expression under abiotic stresses and hormones of the ethylene response factor VII gene FmRAP2.12 from Fraxinus mandshurica. Journal of Forestry Research, 30, 1289–1300.
• Lissarre M, M Ohta, A Sato, and K Miura, 2010. Cold-responsive gene regulation during cold acclimation in plants. Plant Signaling and Behavior. Landes Bioscience, 5, 948-952.
• Liu Q, M Kasuga, Y Sakuma, H Abe, S Miura, K Yamaguchi-Shinozaki, and K Shinozaki, 1998. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell, 10, 1391–1406.
• Mizoi J, K Shinozaki, and K Yamaguchi-Shinozaki, 2012. AP2/ERF family transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1819, 86-96.
• Mosa KA, A. Ismail, and M Helmy, 2017. Introduction to Plant Stresses. Springer, 1–19.
• Palva ET and P Heino, 1997. Molecular Mechanism of Plant Cold Acclimation and Freezing Tolerance. In Plant Cold Hardiness, 3–14.
• Pino MT, A Ávila, A Molina, Z Jeknic, and THH Chen, 2013. Enhanced in vitro drought tolerance of Solanum tuberosum and Solanum commersonii plants overexpressing the ScCBF1 gene. Ciencia e Investigacion Agraria, 40, 171–184.
• Qin F, Y Sakuma, J Li, Q Liu, YQ Li, K Shinozaki, and K Yamaguchi-Shinozaki, 2004. Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L. Plant and Cell Physiology, 45, 1042–1052.
• Riechmann JL and EM Meyerowitz, 1998. The AP2/EREBP family of plant transcription factors. Biological Chemistry. Biological Chemistry, 379, :633-46.
• Shi Y, Y Ding, and S Yang, 2018. Molecular Regulation of CBF Signaling in Cold Acclimation. Trends in Plant Science, 23, 623-637.
• Stockinger EJ, SJ Gilmour, and MF Thomashow, 1997. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proceedings of the National Academy of Sciences of the United States of America, 94, 1035–1040.
• Stockinger EJ, Y Mao, MK Regier, SJ Triezenberg, and MF Thomashow, 2001. Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression. Nucleic Acids Research, 29, 1524–1533.
• Thomashow MF, 2001. So what’s new in the field of plant cold acclimation? Lots! Plant Physiology, 125, 89–93. Yamaguchi-Shinozaki K and K Shinozaki, 1994. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell, 6, 251–264.
• Zarka DG, JT Vogel, D Cook, and MF Thomashow, 2003. Cold Induction of Arabidopsis CBF Genes Involves Multiple ICE (Inducer of CBF Expression) Promoter Elements and a Cold-Regulatory Circuit That Is Desensitized by Low Temperature. Plant Physiology, 133, 910–918.
• Zhang X, SG Fowler, H Cheng, Y Lou, SY Rhee, EJ Stockinger, and MF Thomashow, 2004. Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant. Arabidopsis. Plant Journal, 39, 905–919.