Kekik (Thymus Vulgaris L.) Özütü Kullanarak Biyosentezlenmiş Gümüş Nanopartiküllerin Antibakteriyel Etkisinin Araştırılması

Year 2023, Volume: 23 Issue: 6, 1551 - 1560, 28.12.2023

Cansu Güneş Ahmet Avcı

https://doi.org/10.35414/akufemubid.1289809

Abstract

Nano boyutlu metaller, bakterilere karşı direnç oluşturma noktasında etkili birer antibakteriyel ajan olarak bilinir. Bu ajanlar özellikle insanlık için ciddi bir tehdit oluşturan ilaca dirençli bakterilerle mücadele etmek için doğal kökenli alternatifler oluşturmaktadır. Gümüş nanopartiküllerin (AgNP'ler) iyi derecede antimikrobiyal aktiviteye sahip olduğu kanıtlanmıştır. AgNP'lerin biyosentezi diğer nanopartikül üretim yöntemlerine kıyasla daha güvenli, düşük maliyetli ve çevre dostu bir yöntem olarak kabul edilmektedir. Bu çalışmada, biyosentez yöntemi kullanılarak Thymus Vulgaris L. sulu özütüyle enkapsüle olmuş gümüş nanopartikül (eAgNP) üretimi başarıyla gerçekleştirilmiştir. AgNP’lere kekik bitkisinin antiseptik, antimikrobiyal ve antioksidatif özelliklerinin kazandırılması amaçlanmıştır. eAgNP üretimini sağlayacak optimum bitki özütü ve AgNO3 konsantrasyonu UV-vis spektrofotometre analizi ile belirlenerek bulunan oranlar doğrultusunda yeşil sentez gerçekleştirilmiştir. Sentezlenen eAgNP'ler UV-Vis spektral analizi, FT-IR, XRD analizi ve TEM görüntü analizi ile karakterize edilmiştir. eAgNP'lerin antimikrobiyal aktivitesi E. coli ve S. aureus bakterileri kullanılarak disk difüzyon metodu ile araştırılmıştır. TEM analizi ile biyosentezlenmiş eAgNP'lerin bitki özütü ile enkapsülasyonunun gerçekleştiği ve 6-70 nm boyut aralığında küresel bir şekle sahip olduğu bulunmuştur. eAgNP'leri çevreleyen bitki kaynaklı fitokimyasalların varlığı FT-IR ile, AgNP oluşumu ise XRD analizi ile kanıtlanmıştır. 5 mg/mL'lik bir konsantrasyonda disklere emdirilen eAgNP'ler, E. coli ve S.aureus bakterilerine karşı sırasıyla 20.5 mm ve 23.2 mm ortalama inhibisyon çapları ile antimikrobiyal aktivite göstermiştir. Sonuçlar, oldukça güçlü antimikrobiyal aktiviteye sahip, çevre dostu eAgNP'lerin Thymus Vulgaris L. özütü kullanılarak hazırlanabileceğini ve biyomalzeme alanında önemli bir potansiyel oluşturabileceğini ortaya koymuştur.

Keywords

Antibakteriyel aktivite, Biyosentez, Enkapsülasyon, Gümüş nanopartikül, Thymus vulgaris l.

Supporting Institution

Necmettin Erbakan Üniversitesi Bilimsel Araştırma Projeleri

Project Number

211319018

Thanks

Bu çalışma, Necmettin Erbakan Üniversitesi Bilimsel Araştırma Projeleri tarafından Yüksek Lisans Tez Projesi olarak desteklenen projenin bulgularının bir kısmını içermektedir (Proje No: 211319018).

Investigation of Antibacterial Effect of Biosynthesized Silver Nanoparticles Using Thyme (Thymus Vulgaris L.) Extract

Abstract

Nano-sized metals are known as effective antibacterial agents at the point of creating resistance against bacteria. These agents are alternatives of natural origin to struggle drug-resistant bacteria, which pose a serious threat to humanity. Silver nanoparticles (AgNPs) have been proven to have good antimicrobial activity. Biosynthesis of AgNPs is accepted as a safer, lower cost, and more environmentally friendly method compared to other nanoparticle production methods. In this study, silver nanoparticles (eAgNPs) encapsulated with Thymus Vulgaris L. aqueous extract were successfully produced using the biosynthesis method. It is aimed to bring in the antiseptic, antimicrobial, and antioxidative properties of the thyme plant to AgNPs. The optimum plant extract and AgNO3 concentration that is required for eAgNP production were determined by UV-vis spectrophotometer analysis and green synthesis was carried out in line with the ratios found. The synthesized eAgNPs were characterized by UV-Vis spectral analysis, FT-IR, XRD analysis, and TEM analysis. The antimicrobial activity of eAgNPs was investigated using a disc diffusion method against E. coli and S. aureus. It has been confirmed that the encapsulation of biosynthesized eAgNPs with the plant extract was successfully carried out by TEM analysis and spherical-shaped biosynthesized nanoparticles had a size range of 6-70 nm. The presence of plant-derived phytochemicals surrounding eAgNPs was confirmed by FT-IR and the formation of AgNP was analyzed by XRD spectrum. Biosynthesized eAgNPs adsorbed into discs at a concentration of 5 mg/mL showed antimicrobial activity against E. coli and S. aureus with mean inhibition diameters of 20.5 mm and 23.2 mm, respectively. The results introduced that eco-friendly eAgNPs with strong antimicrobial activity can be prepared using Thymus Vulgaris L. plant extract and may create a significant potential in the field of biomaterials.

Keywords

Antibacterial activity, Biosynthesis, Encapsulation, Silver nanoparticles, Thymus vulgaris l.

Project Number

211319018

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