The Effects of Adding Different Levels of Lactic Acid Bacterial with Enzymes Inoculants on the Fermentation, Aerobic Stability and in vitro Digestibility of Wet Brewers Grain Silages

Abstract

This study was carried out to determine the effect of lactic acid bacteria+enzyme (LAB+E) mixture inoculants on the fermentation, aerobic stability, and in vitro organic matter digestibility (IVOMD) of wet brewers grain silages. SILAID (Global Nutritech Biotechnology LLC, Richmond, VA) containing water soluble Lactobacillus plantarum and Enterococcus faecium bacteria with cellulose, pentosanaz and amylase was used as bacterial inoculants. Inoculants were applied to silages at 5x10⁵, 1x10⁶ and 5x10⁶ cfu/g levels. Wet brewers grains were ensiled in 1.0 litter special jars equipped with a lid that enabled gas release only. Silages were sampled for chemical and microbiological analyses on day 60 after ensiling and subjected to aerobic stability test for 5 days. In addition, IVOMD of silage was determined with enzymatic technique. Lactic acid bacteria+enzyme mixture inoculants decreased the pH, ammonia nitrogen and acetic acid whereas increased the lactic acid and lactobacilli count of silages (P<0.05). In addition, LAB+E mixture inoculants decreased neutral detergent fiber, acid detergent fiber and cellulose contents (P<0.05) whereas were not effect IVOMD of silages (P>0.05). 

Keywords

• Wet brewers grain,fermentation,aerobic stability

Farklı Düzeylerde Laktik Asit Bakterileri ile Enzim İlavesinin Yaş Bira Posası Silajlarında Fermantasyon, Aerobik Stabilite ve in vitro Sindirim Üzerine Etkileri

Öz

Bu çalışma, laktik asit bakteri+enzim (LAB+E) karışımı inokulantın yaş bira posası silajlarının fermantasyon ve aerobik stabilite özellikleri ile in vitro organik madde sindirilebilirliği (IVOMS) üzerindeki etkilerinin belirlenmesi amacıyla düzenlenmiştir. İnokulant olarak, Lactobacillus plantarum ve Enterococcus faecium bakterileri ile birlikte selülaz, pentozanaz ve amilaz enzimlerini içeren SILAID (Global Nutritech Biotechnology LLC, Richmond, VA) kullanılmıştır. İnokulant silajlara 5x10⁵, 1x10⁶ ve 5x10⁶ kob g^-1 düzeylerinde ilave edilmiştir. Yaş bira posaları yalnızca gaz çıkışına izin veren 1 litrelik cam kavanozlara silolanmışlardır. Altmış günlük silolanma süresi sonrasında açılan silajlarda kimyasal ve mikrobiyolojik analizler yapılmış ve silajlar 5 gün süreyle aerobik stabilite testine tabi tutulmuştur. Ayrıca, silajların enzimatik yöntem ile IVOMS saptanmıştır. Sonuç olarak, LAB+E inokulantı ilavesi silajların pH, amonyak azotu ve asetik asit içeriklerini düşerken, laktik asit içeriklerini ve lactobacilli sayısını artırmıştır (P<0.05). Ayrıca LAB+E ilavesi silajların nötr deterjanda çözünmeyen lif,  asit deterjanda çözünmeyen lif ve selüloz içeriğini azaltmıştır (P<0.05), bunlara karşın IVOMS etkilememiştir (P>0.05).

Anahtar Kelimeler

• Yaş bira posası,fermantasyon,aerobik stabilite

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