Intra-Genetic Variation within Olive Cultivar 'Nabali' in Palestine by Microsatellite and Random Amplified Polymorphic DNA

Abstract

ABSTRACT: Over seventy olive trees (Nabali) from different regions in Palestine were used in this study. Intra-genetic variation within different olive Nabali variants were approved by SSR and RAPD markers. Four SSRs bands were monomorphic revealing a true-to-genotype of Nabali cultivar. Ten RAPD markers produced 60 reproducible bands with an average of 6 bands / marker. Only 24 were polymorphic. The percentage of polymorphic bands was 38% which is relatively high. Similarity matrix for studied populations ranged from moderate (0.610) for Jalkamous and Karawa Bani Zaid (2) to highly genetic similarity or even identity (1.000) in some cases as Aqraba and Aseerah (N).  The interaction between different variants trends to be high. The effect of geographic location was absent in this study and has no significant contribution. Dendogram based on Jaccards coffiecnt  revealed three main clusters, the biggest group consisted of the majority of variants including Bieta, Karawa Bani Zaid (1), Salfeet, Salfeet (h), Aqraba, Aseereh (N), Jalkamous, and Alaroub. Second group consisted of two variants Alaar and Nahaleen. The third group is containing only Karawa Bani Zaid (2). The relative high polymorphic bands of RAPD markers (40%) and moderate genetic similarity among different Nabali variants suggested attribution of genetic background. Selection for new traits within Nabali is suggested.

 

Keywords

• Olive,Intra-Genetic Variation,SSR,RAPD.

Intra-Genetic Variation within Olive Cultivar 'Nabali' in Palestine by Microsatellite and Random Amplified Polymorphic DNA

Abstract

ABSTRACT: Over seventy olive trees (Nabali) from different regions in Palestine were used in this study. Intra-genetic variation within different olive Nabali variants were approved by SSR and RAPD markers. Four SSRs bands were monomorphic revealing a true-to-genotype of Nabali cultivar. Ten RAPD markers produced 60 reproducible bands with an average of 6 bands / marker. Only 24 were polymorphic. The percentage of polymorphic bands was 38% which is relatively high. Similarity matrix for studied populations ranged from moderate (0.610) for Jalkamous and Karawa Bani Zaid (2) to highly genetic similarity or even identity (1.000) in some cases as Aqraba and Aseerah (N).  The interaction between different variants trends to be high. The effect of geographic location was absent in this study and has no significant contribution. Dendogram based on Jaccards coffiecnt  revealed three main clusters, the biggest group consisted of the majority of variants including Bieta, Karawa Bani Zaid (1), Salfeet, Salfeet (h), Aqraba, Aseereh (N), Jalkamous, and Alaroub. Second group consisted of two variants Alaar and Nahaleen. The third group is containing only Karawa Bani Zaid (2). The relative high polymorphic bands of RAPD markers (40%) and moderate genetic similarity among different Nabali variants suggested attribution of genetic background. Selection for new traits within Nabali is suggested.

 

Keywords

• Olive,Intra-Genetic Variation,SSR,RAPD.

References

1. Abdelhamid, S., N. Grat-Kamoun, F. Marra, and T. Caruso. 2012. Genetic similarity among Tunisian cultivated olive estimated through SSR markers. Scientia Agricola 70: 33-38.
2. Angiolillo, A., M. Mencuccini, L. Baldoni. 1999. Olive genetic diversity assessed using amplified fragment length polymorphisms. Theoretical and Applied Genetics 98: 411-421.
3. Belaj, A., Z. Satovic, G. Cipriani, L. Baldoni, R. Testolin, L. Rallo, I. Trujillo. 2003. Comparative study of the discriminating capacity of RAPD AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive. Theoretical and Applied Genetics 107: 736-744.
4. Belaj, A., Z. Satovic, L. Rallo, I. Trujillo. 2004. Optimal use of RAPD markers for identifying varieties in olive (Olea europaea L.) germplasm collections. Journal of the American Society for Horticultural Science 129: 266-270.
5. Cipriani, G., M. T. Marrazzo, R. Marconi, A. Cimato, R. Testolin. 2002. Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theoretical and Applied Genetics 104 (2-3): 223-228.
6. Gomes, S., P. Martins-Lopes, J. Lima-Brito, J. Meirinhos, J. Lopes, A. Martins, H. Guedes-Pinto. 2008. Evidence of clonal variation in olive ‘Verdeal-Transmontana’ cultivar using RAPD, ISSR and SSR markers. Journal of Horticultural Science and Biotechnology 83: 395-400.
7. Gomes, S., P. Martins-Lopes., H. Guedes-Pinto. 2012. Olive tree genetic resources characterization through molecular markers. In: Mahmut Caliskan (Ed.), Genetic Diversity in Plants, ISBN: 978-953-51-0185-7, InTech, DOI: 10.5772/32973. Available from: http://www.intechopen.com/books/genetic-diversity-in-plants/olive-tree-genetic-resources-characterization-through-molecular-markers.
8. Ipek, A., E. Barut, H. Gulen, M. Ipek. 2012. Assessment of inter and intra-cultivar variations in olive using SSR markers. Sci. Agric. 69: 327-335.

9. Jaccard P. 1908. Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles 44: 223-270.
10. Khadari, B., C. Breton, N. Moutier, J. Roger, G. Besnard, A. Berville, F. Dosba. 2003. The use of molecular markers for germplasm management in a French olive collection. Theoretical and Applied Genetics 106: 521-529.
11. Muzzalupo, I., A. Chiappettac, C. Benincasaa, E. Perri. 2010. Intra-cultivar variability of three major olive cultivars grown in different areas of central-southern Italy and studied using microsatellite markers. Scientia Horticulturae 126: 324-329.
12. Owen, C., A. Carolyn, L. Bita, G. Banilas, S. Hajjar, V. Sellianakis, U. Aksoy, S. Hepaksoy, R. Chamoun R., S. Talhook., I. Metzidakis, P. Hatzopoulos, P. Kalaitzis. 2005. AFLP reveals structural details of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean. Theoretical and Applied Genetics 110: 1169-1176.
13. Qutub, M., S. Ali, M. Mutawea, M. Abed, T. Arabasi, F. Pierini, E. Lodolini. 2010. Characterization of the main Palestinian olive cultivars and olive oil.
14. Salomonson, A. 1996. Interactions between somatic mutations and plant development. Vegetatio 127: 71-75.
15. Schlüter, P. M., S. A. Harris. 2006. Analysis of multilocus fingerprinting data sets containing missing data. Mol. Ecol. Notes. 6: 569-572.
16. Wiesman, Z., A. Avidan, S. Lavee, B. Quebedaux.1998. Molecular characterization of common olive varieties in Israel and the West bank using randomly amplified polymorphic DNA (RAPD) markers. J. Amer. Soc. Hort. Sci. 123: 837-841.
17. Zohary, D., and M. Hopf. 1994. Domestication of plants in the Old World: The origin and spread of cultivated plants in west Asia, Europe, and the Nile Valley. 2nd ed. Oxford Clarendon Press.