Intra-Genetic Variation within Olive Cultivar 'Nabali' in Palestine by Microsatellite and Random Amplified Polymorphic DNA
Öz
ABSTRACT:
Over seventy olive trees (Nabali) from different regions in Palestine were used
in this study. Intra-genetic variation within different olive Nabali variants
were approved by SSR and RAPD markers. Four SSRs bands were monomorphic
revealing a true-to-genotype of Nabali cultivar. Ten RAPD markers produced 60
reproducible bands with an average of 6 bands / marker. Only 24 were
polymorphic. The percentage of polymorphic bands was 38% which is relatively
high. Similarity matrix for studied populations ranged from moderate (0.610)
for Jalkamous and Karawa Bani Zaid (2) to highly genetic similarity or even
identity (1.000) in some cases as Aqraba and Aseerah (N). The interaction between different variants
trends to be high. The effect of geographic location was absent in this study
and has no significant contribution. Dendogram based on Jaccards coffiecnt revealed three main clusters, the biggest
group consisted of the majority of variants including Bieta, Karawa Bani Zaid
(1), Salfeet, Salfeet (h), Aqraba, Aseereh (N), Jalkamous, and Alaroub. Second
group consisted of two variants Alaar and Nahaleen. The third group is
containing only Karawa Bani Zaid (2). The relative high polymorphic bands of
RAPD markers (40%) and moderate genetic similarity among different Nabali
variants suggested attribution of genetic background. Selection for new traits
within Nabali is suggested.
Anahtar Kelimeler
Kaynakça
- Abdelhamid, S., N. Grat-Kamoun, F. Marra, and T. Caruso. 2012. Genetic similarity among Tunisian cultivated olive estimated through SSR markers. Scientia Agricola 70: 33-38.
- Angiolillo, A., M. Mencuccini, L. Baldoni. 1999. Olive genetic diversity assessed using amplified fragment length polymorphisms. Theoretical and Applied Genetics 98: 411-421.
- Belaj, A., Z. Satovic, G. Cipriani, L. Baldoni, R. Testolin, L. Rallo, I. Trujillo. 2003. Comparative study of the discriminating capacity of RAPD AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive. Theoretical and Applied Genetics 107: 736-744.
- Belaj, A., Z. Satovic, L. Rallo, I. Trujillo. 2004. Optimal use of RAPD markers for identifying varieties in olive (Olea europaea L.) germplasm collections. Journal of the American Society for Horticultural Science 129: 266-270.
- Cipriani, G., M. T. Marrazzo, R. Marconi, A. Cimato, R. Testolin. 2002. Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theoretical and Applied Genetics 104 (2-3): 223-228.
- Gomes, S., P. Martins-Lopes, J. Lima-Brito, J. Meirinhos, J. Lopes, A. Martins, H. Guedes-Pinto. 2008. Evidence of clonal variation in olive ‘Verdeal-Transmontana’ cultivar using RAPD, ISSR and SSR markers. Journal of Horticultural Science and Biotechnology 83: 395-400.
- Gomes, S., P. Martins-Lopes., H. Guedes-Pinto. 2012. Olive tree genetic resources characterization through molecular markers. In: Mahmut Caliskan (Ed.), Genetic Diversity in Plants, ISBN: 978-953-51-0185-7, InTech, DOI: 10.5772/32973. Available from: http://www.intechopen.com/books/genetic-diversity-in-plants/olive-tree-genetic-resources-characterization-through-molecular-markers.
- Ipek, A., E. Barut, H. Gulen, M. Ipek. 2012. Assessment of inter and intra-cultivar variations in olive using SSR markers. Sci. Agric. 69: 327-335.
- Jaccard P. 1908. Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles 44: 223-270.
- Khadari, B., C. Breton, N. Moutier, J. Roger, G. Besnard, A. Berville, F. Dosba. 2003. The use of molecular markers for germplasm management in a French olive collection. Theoretical and Applied Genetics 106: 521-529.