In Vitro Plant Regeneration of Libyan Wild Plants: Edible Species (Arbutus pavarii) and Endanger Species [Haplophyllum tuberculatum (Forsk.) Juss]

Year 2017, Volume: 27 Issue: 2, 127 - 132, 15.12.2017

Adel M. Elmaghabı Salem Hammud Elmunder Abugnıa

Abstract

Protocol of
micropropagation of Arbutus pavarii and Haplophyllum
tuberculatum was successfully achieved. In vitro plant
regeneration of Arbutus pavarii was attempted using single nodal on MS
medium supplemented with different concentration of three various growth
regulators indole-3-butyric acid (IBA), Kinetin (K) and
N-Isopentenylaminopurine (2ip). Multiple shoots from single node were obtained
on MS medium with the 0.5 mg/l 2ip. While, faster improving of shoots elongation
was on MS contained 0.5 mg/lK. Explants obtained in
micropropagation step were used for rooting step under several treatments. The
best results were obtained when explants were sup-cultured on MS medium with 1
mg/l IBA. New plants were vigorous, of good quality and presented phenotypic
characters similar to mother plants. Micropropagation of Haplophyllum tuberculatum was achieved from sterilized single
nodal segments on MS medium supplemented with for different concentrations of
2,4-D for callus induction and other different hormones K and BA for
multiplication of auxiliary branches and rooting . The highest results of the
weight of callus were growing in MS medium containing 1 or 2 mg/l 2.4-D hormone
with maltose. Whereas, the axillary soothing was significantly proliferated on
MS medium supplemented 2 mg/ l^-1 K. Acclimation of plantlet was in
greenhouse. 

Keywords

Micropropagation, growth regulators, Arbutus pavarii, Haplophyllum tuberculatum, in vitro culture

References

• Al-Rehaily, A. J., S. I. Alqasoumi, H. S. Yusufoglu, M. A. Al-Yahya, B. Demirci, N. Tabanca, D. E. Wedge, F. Demirci, U. R. Bernier, J. Becnel, H. E. Temel, and K. H. Baser. 2014. Chemical composition and biological activity of Haplophyllum tuberculatum Juss. essential oil. Journal of Essential Oil Bearing Plants 17 (3): 452-459.
• Bertsouklis, K. F., and M. Papafotiou. 2009. In vitro propagation of Arbutus andrachne L. Acta Hort. 813: 477-479.
• El-Darier, S. M., and F. M. El-Mogaspi. 2009. Ethnobotany and relative importance of some endemic plant species at El-Jabal El-Akhdar Region (Libya). World Journal of Agricultural Sciences 5 (3): 353-360.
• Elmaghrabi, A. M., and S. J. Ochatt. 2006. Isoenzymes and flow cytometry for the assessment of true-to-typeness of calluses and cell suspension of barrel medic prior to regeneration. Plant Cell Tissue Org. Cult. 85: 31-43.
• Elmaghrabi, A. M., E. Abughnia, and S. Hamoud. 2017. In vitro propagation of the wild medicinal plant, caper (Capparis spinosa L.). African Journal of Biotechnology. ISSN: 1684-5315. http://www. academicjournals.org/AJB. (under press).
• El-Naggar, E. B., S. M. El-Darier, A. A. El-Mekanen, E. Švajdlenka, and M. Zemlièka,. 2014. Chemical Composition of Essential Oil of Haplophyllum tuberculatum (Rutaceae) Grow Wild in Different Habitats of Egypt. Global Journal of Pharmacology 8 (3): 385-393.
• George, E. F., M. A. Hall, and G. J. De Klerk. 2008. Plant Propagation by Tissue Culture, 3rd Edition. Springer.
• Gonçalves, J. C., G. Diogo, and S. Amâncio. 1998. In vitro propagation of chestnut (Castanea sativa × C. crenata): Effects of rooting treatments on plant survival, peroxidase activity and anatomical changes during adventitious root formation. Sci. Hort. 72 (3): 265-275.
• Gholami, M., K. Javidnia, R. Miri, and M. Soltani. 2009. Micropropagated Haplophyllum patavinum Hand-Mazz. (Rutaceae) Oil as rich source of α-Palmitolactone. Iranian Congress of Physiology and Pharmacology (19). http://en.seminars.sid.ir/View Paper.aspx?ID=1607.
• Louhaichi, M., A. K. Salkini, H. E. Estita, and S. Belkhir. 2011. Initial Assessment of Medicinal Plants Across the Libyan Mediterranean Coast. Advances in Environmental Biology 5 (2): 359-370.
• Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
• Puricelli, L., G. Innocenti, G. Dellemonache, R. Caniato, R. Filippini, and E. M. Cappelletti. 2002. In vivo and in vitro production of alkaloids by Haplophyllum patavinum. Natural Product Letters 16 (2): 95-100.
• Torres, J. A., F. Valle, C. Pinto, A. Gar cia-Fuentes, C. Salazar. And E. Cano. 2002. Arbutus unedo L. communities in southern Iberian Peninsula mountains. Plant Ecol. 160: 207-223.