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Tütsülenmiş ve Marine Edilmiş Gökkuşağı Alabalığı (Oncorhynchus mykiss) Filetolarının Soğutulmuş Saklama Süresince Elektroforetik Protein Profilindeki Değişimler

Year 2015, Volume: 21 Issue: 2, 262 - 269, 11.03.2015
https://doi.org/10.15832/tbd.29146

Abstract

Bu çalışmayla, tütsülenmiş ve marine edilmiş gökkuşağı alabalığının (Oncorhynchus mykiss) buzdolabında depolama
süresince elektroforetik protein profilindeki değişimlerin belirlenmesi amaçlanmıştır. Çiğ, tütsülenmiş ve marine edilmiş
alabalık örneklerinin, 9 haftalık buzdolabında depolama süresince kas proteinlerindeki değişimler sodyum dodesil sülfat
poliakrilamid jel elektroforezi (SDS-PAGE) kullanılarak incelenmiştir. SDS-PAGE ve densitometrik analiz sonuçlarına
göre, tütsülenmiş ve marine edilmiş alabalık kaslarının, aktin bandlarında önemli bir değişimin olmadığı, buna karşılık
bazı protein banlarının sayısında ve yoğunluğunda bir azalmanın meydana geldiği gözlemlenmiştir. Depolamanın
9. haftasında, 205 kDa molekül ağırlığındaki myosin ağır zinciri (MHC), gözden kaybolmuştur. Ayrıca tütsülenmiş
ve marine edilmiş örneklerin protein yüzdeleri de haftalık olarak önemli oranda değişim göstermiştir (P

References

  • Alparslan Y, Baygar T, Hasanhocaoğlu H & Metin C (2013). Effects of scale and skin on chemical and sensory quality of marinated sea bass filets (Dicentrarchus labrax, L. 1758) in sunflower oil during storage at 4 ºC. Emirates Journal of Food and Agriculture 25(7): 516-523
  • An H, Marshall M R, Otwell W S & Wei C I (1988). Electrophoretic identification of raw and cooked shrimp using various protein extraction system. Journal of Food Science 53(2): 313-318
  • AOAC (1990). Official methods of analysis, 15th ed. Association of the Offical Analtical Chemists, Washington, DC, USA
  • Ayinsa H K & Maalekuu B K (2013). Effect of traditional fish processing methods on the proximate composition of red fish stored under ambient room conditions. American Journal of Food and Nutrition 3(3): 73-82
  • Basti A A, Misaghi A, Salehi T Z & Kamkar A (2006). Bacterial pathogens in fresh, smoked and salted Iranian fish. Food Control 17(3): 183-188
  • Benjakul S & Bauer F (2000). Physicochemical and enzymatic changes of cod muscle proteins subjected to different freze ±thaw cycles. Journal of the Science of Food and Agriculture 80: 1143-1150
  • Clucas I J & Ward A R (1996). Post-Harvest Fisheries Development: A guide to Handling, Preservation, Processing and Quality. Chathman Maritime, Kent ME44TB, United Kingdom
  • Cosansu S, Mol S, Ucok Alakavuk D & Tosun Ş Y (2011). Effects of Pediococcus spp. on the Quality of Vacuum- Packed Horse Mackerel during Cold Storage. Tarım

Changes of Electrophoretic Protein Profiles of Smoked and Marinated Rainbow Trout (Oncorhynchus mykiss) During Refrigerated Storage

Year 2015, Volume: 21 Issue: 2, 262 - 269, 11.03.2015
https://doi.org/10.15832/tbd.29146

Abstract

In this study, we aimed to determine the changes of electrophoretic protein profiles of smoked and marinated rainbow trout (Oncorhynchus mykiss) during refrigerated storage. Changes in muscle proteins during 9 weeks refrigerated storage of raw, smoked and marinated trout samples have been examined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE and densitometric analysis revealed that intensity and the number of some protein bands were reduced while the bands of actin didn’t change significantly during marination and smoking of rainbow trout muscle. Myosin heavy chain (MHC) band with molecular weight of 205 kDa disappeared in the 9th week of smoking and marinating process. Subsequently, there were significant weekly changes in the protein percentages of samples caused by smoking and marinating processes (P<0.01). Protein percentages of the raw, smoked and marinated samples were determined as 18.34-18.82%, 23.83-24.70% and 16.29-17.76%, respectively

References

  • Alparslan Y, Baygar T, Hasanhocaoğlu H & Metin C (2013). Effects of scale and skin on chemical and sensory quality of marinated sea bass filets (Dicentrarchus labrax, L. 1758) in sunflower oil during storage at 4 ºC. Emirates Journal of Food and Agriculture 25(7): 516-523
  • An H, Marshall M R, Otwell W S & Wei C I (1988). Electrophoretic identification of raw and cooked shrimp using various protein extraction system. Journal of Food Science 53(2): 313-318
  • AOAC (1990). Official methods of analysis, 15th ed. Association of the Offical Analtical Chemists, Washington, DC, USA
  • Ayinsa H K & Maalekuu B K (2013). Effect of traditional fish processing methods on the proximate composition of red fish stored under ambient room conditions. American Journal of Food and Nutrition 3(3): 73-82
  • Basti A A, Misaghi A, Salehi T Z & Kamkar A (2006). Bacterial pathogens in fresh, smoked and salted Iranian fish. Food Control 17(3): 183-188
  • Benjakul S & Bauer F (2000). Physicochemical and enzymatic changes of cod muscle proteins subjected to different freze ±thaw cycles. Journal of the Science of Food and Agriculture 80: 1143-1150
  • Clucas I J & Ward A R (1996). Post-Harvest Fisheries Development: A guide to Handling, Preservation, Processing and Quality. Chathman Maritime, Kent ME44TB, United Kingdom
  • Cosansu S, Mol S, Ucok Alakavuk D & Tosun Ş Y (2011). Effects of Pediococcus spp. on the Quality of Vacuum- Packed Horse Mackerel during Cold Storage. Tarım
There are 8 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Makbule Baylan

Makbule Baylan

Gamze Mazı

Numan Özcan

Bahri Özcan This is me

Mustafa Akar This is me

Ali Coşkun

Publication Date March 11, 2015
Submission Date January 7, 2014
Published in Issue Year 2015 Volume: 21 Issue: 2

Cite

APA Baylan, M., Baylan, M., Mazı, G., Özcan, N., et al. (2015). Changes of Electrophoretic Protein Profiles of Smoked and Marinated Rainbow Trout (Oncorhynchus mykiss) During Refrigerated Storage. Journal of Agricultural Sciences, 21(2), 262-269. https://doi.org/10.15832/tbd.29146

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