Gamma rays and EMS induced Flower Color Mutation in Grasspea (Lathyrus sativus Linn.)

Year 2017, Volume: 23 Issue: 4, 423 - 427, 18.01.2017

Pegah Ramezani Morteza Siavoshi , Anil D More Mahboubeh Ebrahimi

https://doi.org/10.15832/ankutbd.385866

Cited By: 1

Abstract

To induce flower color mutants in Grasspea (Lathyrus sativus Linn.) variety “Pusa-24“, the fresh seeds were exposed to 4 different doses of gamma radiation as physical mutagens (100, 200, 300 and 400 Gy) and treated with 4 different concentration of Ethyl Methanesulphonate as chemical mutagen (5, 10, 15 and 20 mM ) and also combination of both mutagens. Due to mutagenesis broad range of flower color mutants in M₂, M₃ and M₄ were obtained. Various unique and different flower color mutations like pink, purple, white and yellow detected against blue which is natural color in control progenies. Maximum flower color frequency was recorded in EMS treated seeds (20 mM) in 4th generation and lowest mutation frequency in 2nd generation was obtained in gamma rays (100 Gy) treated seeds. The results showed more flower color mutations happened in more dose /concentration of mutagens in every generation

Keywords

Flower color, Mutation, Grasspea, Gamma radiation, EMS