Year 2018, Volume 24 , Issue 2, Pages 269 - 277 2018-06-01

Proteolysis in the Beyaz (White) Cheese Produced From Various Milk

Zübeyde Öner [1] , Mine Sarıdağ [2]


The aim of this study was to investigate proteolysis development and peptide changes during the storage period of Beyaz (White) cheese which is produced from various types of milk, such as sheep, goat and cow milk. Three types of cheese were produced using goat, sheep and cow milk, and all analyses were performed in duplicate. Proteolytic changes were observed in cheese on the 1st day, 3rd, 6th, and 9th month. The total protein was investigated through using water-soluble nitrogen, soluble nitrogen in 12% Trichloroacetic acid (TCA), soluble nitrogen in 5% Phosphotungstic acid (PTA), and free amino acids. Proteolytic changes and peptide formations were observed during maturation by HPLC. While the total protein was 14.33% in sheep Beyaz cheese on the 1st day, this value decreased during the maturation period and reduced to 6.9% in the 9th month. These values were 13.55 and 7.95 for cow cheese and 16.30% and 7.95% for goat cheese, respectively. The water-soluble protein value increased during the maturation period. The value was 1.41% in sheep cheese on the 1st day, and increased to 6.24% in the 9th month. These values were 2.16% and 4.92% for goat cheese, and 1.79% and 8.53% for cow cheese, respectively. 12% TCA soluble nitrogen had been 0.211% at the beginning and was 0.51% in the 9th month. The ripening value based on 12% TCA changed between 9.41%-47.22%. 5% PTA soluble nitrogen changed between 0.075%- 0.25 %. The ripening value based on 5% PTA changed between 3.34-23.14. Changes in concentration of total free amino acids during cheese ripening were tracked. In water soluble extracts of cheese, the presence of free amino

 groups in all ripening stages was detected. It is observed that amino acids and smaller peptides-have concentration significantly (P<0.05) increased during ripening. The total free amino acid was found as 0.24 mg lysine g-1 in sheep cheese, 0.215 mg lysine g-1 in goat cheese and 0.208 mg lysine g-1 in cow cheese at the end of ripening period. 


Proteolysis; Sheep milk cheese; Goat milk cheese; Cow milk cheese
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Primary Language en
Subjects Engineering
Journal Section Makaleler
Authors

Author: Zübeyde Öner (Primary Author)
Institution: Suleyman Demirel University, Engineering Faculty, Food Engineering Department Isparta, TURKE
Country: Turkey


Author: Mine Sarıdağ
Institution: Suleyman Demirel University, Engineering Faculty, Food Engineering Department Isparta, TURKE
Country: Turkey


Dates

Application Date : August 19, 2016
Acceptance Date : January 18, 2018
Publication Date : June 1, 2018

EndNote %0 Journal of Agricultural Sciences Proteolysis in the Beyaz (White) Cheese Produced From Various Milk %A Zübeyde Öner , Mine Sarıdağ %T Proteolysis in the Beyaz (White) Cheese Produced From Various Milk %D 2018 %J Journal of Agricultural Sciences %P -2148-9297 %V 24 %N 2 %R doi: 10.15832/ankutbd.446454 %U 10.15832/ankutbd.446454