Effect of Combined or Separate Administration of Beta Carotene-Vitamin E and hCG on Fertility in Sheep Lambs

Year 2022, Volume: 28 Issue: 3, 396 - 400, 05.09.2022

M. Ferit Özmen , Erkan Say , Ümüt Cirit

https://doi.org/10.15832/ankutbd.932413

Cited By: 2

Abstract

This study was performed to investigate the effect of beta carotene-vitamin E and hCG treatments alone or a combination of both on fertility in estrus synchronized Awassi ewe lambs. A total of 103 Awassi ewe lambs were divided into four groups before the study. Lambs were treated with a progesterone sponge for 12 days, PGF2α two days before sponge removal, 600 IU PMSG on sponge removal day, and 150 IU hCG on the day of mating. The control group (n: 25) did not receive any additional treatment. The Vitamin group (β carotene + vitamin E) (n: 26) was treated twice with vitamin combination. The first treatment was on the 7th day before the sponge insertion and the second treatment was on the day of mating. The hCG group (n: 24) was treated with 150 IU hCG on day 12 after mating. The HCG + vitamin group (n: 28) was treated with both β carotene-vitamin E and hCG. Ewe lambs standing to be mounted were considered in estrus and mated. Pregnancy was determined by ultrasound the 30th day after the mating. There were no significant differences between the control and hCG, vitamin and hCG + vitamin groups concerning estrus, conception, lambing, abortion, twinning, fecundity rate, and litter size (P>0.05). It was concluded that the treatments with β-carotene-vitamin E and hCG or both, in addition to estrus synchronization out of the breeding season in Awassi ewe lambs did not improve the investigated fertility indices.

Keywords

Beta carotene, Estrus synchronization, Ewe lambs, hCG, Progesterone

References

• 1. Afshari G, Hasanpoor A, Hagpanah H & Amoughll-Tabrizi A (2008). Seosonal variation of vitamin A and β-carotene levels in Ghezel Sheep. Turkish Journal of Veterinary and Animal Sciences 32 (2): 127-129.
• 2. Ahlswede L & Lotthammer KH (1978). Untersuchungen über eine spezifische, Vitamin A- unabhängige Wirkung des β-Carotins auf die Fertilität des Rindes: 5. Mitteilung: Organuntersuchnungen (Ovarien, Corpora lutea, Leber, Fettgewebe, Uterussekret, Nebennieren) Gewichts und Gehaltsbestimmungen. Deutsche Tierärztliche Wochenschr 85: 7-12 (in German language).
• 3. Arıkan S & Muğlalı, ÖH (1999). Bazı çiftlik hayvanlarının üreme fonksiyonları üzerine B-karotenin etkisi. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 39(2), 87-94.
• 4. Ashworth CJ & Bazer FW (1989). Changes in Ovine Conceptus and Endometrial Function Following Asynchronus Embryo Transfer or Administration of Progesterone. Biology of Reproduction 40(2), 425-433.
• 5. Ayaşan T & Karakozak E (2010). "Hayvan beslemede β-karoten kullanılması ve etkileri. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16.4 : 697-705.
• 6. Bazer FW, Ott TL & Spencer TE (1998). Maternal recognition of pregnancy: comparative aspects a review. Trophoplast Research 12: 375-386.
• 7. Beck NFG, Peters AR & Williams SP (1994). The effect of GnRH agonist (buserelin) treatment on day 12 post mating on the reproductive performance of ewes. Animal Science 58 (2): 243-247.
• 8. Beck NF, Jones M, Davies B, Mann GE & Peters AR (1996). The effect of GnRH analogue (buserelin) treatment on day 12 post mating on ovarian structure and plasma progesterone and oestradiol concentration in ewes. Animal Science 63 (3): 407-412.
• 9. Beytut E, Kamiloğlu NN & Gökçe G (2005). Season variation of vitamin A, E and Beta Carotene levels in plasma of Sheeps and Meadow Hay in the region of Kars and its surrounds. Kafkas Üniversitesi Veteriner Fakültesi Dergisi; 11 (1): 17-24.
• 10. Bolet G (1986). Timing and extent of embryonic mortality in pigs, sheep and goats: genetic variability. In: Sreenan, J.M., Diskin, M.G. (Eds.), Embryonic Mortality in Farm Animals. Martinus Nijhoff, Hague.
• 11. Brief S & Chew BP (1985). Effects of vitamin A and β-carotene on reproductive performance in gilts. Journal of Animal Science 60 (4): 998-1004.
• 12. Cam MA, Kuran M, Yildiz S & Selcuk E (2002). Fetal growth and reproductive performance in ewes administered GnRH agonist on day 12 post-mating. Animal Reproduction Science 72 (1-2), 73-82.
• 13. Cam MA & Kuran M (2004). Effects of a single injection of hCG or GnRH agonist on day 12 post mating on fetal growth and reproductive performance of sheep. Animal Reproduction Science 80 (1-2): 81-90.
• 14. Catalano MT, González C, Williams S, Videla DI & Callejas S (2015). Reproductive performance of ewe lambs in non-breeding season exposed to hCG at day 12 post mating. Small Ruminant Research 124: 63-67.
• 15. Ceylan A, Serin İ, Akşit H, Seyrek K & Gökbulu TC (2007). Investigation of Vitamins A, E, beta-carotene, cholesterol and triglyceride concentrations in dairy cows with repeat breeder and anestrus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 13 (2), 143-147.
• 16. Cole LA (2012). hCG, the wonder of today’s science. Reproductive Biology and Endocrinology 10: 24.
• 17. Fernandez J, Bruno-Galarraga MM, Soto AT, de la Sota RL, Cueto MI, Lacau IM & Gibbons AE (2018). Hormonal therapeutic strategy on the induction of accessory corpora lutea in relation to follicle size and on the increase of progesterone in sheep. Theriogenology 105: 184-188.
• 18. Gabryszuk M & Klewiec J (2002). Effect of injecting 2-and 3-year-old ewes with selenium and selenium–vitamin-E on reproduction and rearing of lambs. Small Ruminant Research 2002; 43 (2): 127-132.
• 19. Gordon IR. (1997). Controlled reproduction in sheep and goats. Cab. International.(2) Ireland, 450.
• 20. Graves-Hoagland RL, Hoagland TA & Woody CO (1989). Relationship of plasma β-carotene and vitamin A to postpartum cattle. Journal of Dairy Science 72 (7): 1854-1858.
• 21. Hunter MG, Southee JA, Mc Leod BJ & Haresign W (1986). Progesterone treatment has a direct effect on GnRH induced preovulatory follicles to determine their ability to develop into normal corpora lutea in anoestrous ewes. Journal of Reproduction and Fertility 76: 349-363.
• 22. Hunter MG, Southee JA & Lamming GE (1988). Function of abnormal corpora lutea in vitro after GnRH induced ovulation in the anoestrous ewe. Journal of Reproduction and Fertility 84: 139-148.
• 23. Ishida N, Okada M, Sebata K, Minato M & Fukui Y (1999). Effects of GnRH and hCG treatment for enhancing corpus lu-teum function to increase lambing rate of ewes artificially inseminated during the non-breeding season. Journal of Reproduction and Development 45: 73-79.
• 24. Kaçar C, Kamiloğlu NN, Gürbulak K, Pancarcı ŞM, Güngör Ö, Guevenc K & Saban, E (2008). The Effect of administration of testosterone antibody, β-carotene and vitamin E on multiple pregnancy and MDA (Malondialdehyde) in Tuj breed sheep in non-breeding. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 14 (1): 51-56.
• 25. Kaya S, Kaçar C, Kaya D & Aslan S (2013). The effectiveness of supplemental administration of progesterone with GnRH, hCG and PGF2α on the fertility of Tuj sheep during the non-breeding season. Small Ruminant Research 113 (2-3): 365-370.
• 26. Khan TH, Beck NF & Khalid M (2007). The effects of GnRH analogue (buserelin) or hCG (Chorulon) on Day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. Animal Reproduction Science 102 (3-4): 247-257.
• 27. Khan TH, Beck NFG & Khalid M (2009). The effect of hCG treatment on Day 12 post-mating on ovarian function and reproductive performance of ewes and ewe lambs. Animal Reproduction Science 116 (1-2): 162-168.
• 28. Kormann AW, Riss G & Weiser H (1989). Improved reproductive performance of rabbit does supplemented with dietary beta-carotene. The Journal of Applied Rabbit Research 12:15-21
• 29. Kott RW, Ruttle JL & Southward GM (1983). Effects of vitamin E and selenium injections on reproduction and preweaning lamb survival in ewes consuming diets marginally deficient in selenium. Journal of Animal Science 57 (3): 331-337.
• 30. Koyuncu M & Yerlikaya H (2007). Effect of selenium–vitamin E injections of ewes on reproduction and growth of their lambs. South African Journal of Animal Science 37 (4): 233-236.
• 31. Köse M, Kırbaş M, Dursun Ş & Bayrıl T (2013). The effect of injections of β-carotene or vitamin E + selenium on fertility in ewes in anestrus season. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 24 (2): 83-86.
• 32. Kumagai H & White CL (1995). The effect of supplementary minerals, retinol and α-tocopherol on the vitamin status and productivity of pregnant merino ewes. Australian Journal of Agricultural Research 46 (6): 1159-1174.
• 33. Mann GE & Picton HM (1995). Ovarian and uterine effects of a single buserelin injection on day 12 of the oestrous cycle in the cow. Journal of reproduction and fertility Abstr. Ser. Vol. 15.
• 34. McMillan WH, Knight TW & Macmillan KL (1986). Effects of gonadotrophin releasing hormone (buserelin) on sheep fertility. Proceedings of the New Zealand Society of Animal Production 46: 161-163.
• 35. Mehri R, Rostami B, Masoumi R & Shahir MH (2018). Effect of injection of GnRH and hCG on day 5 post mating on maternal P4 concentration and reproductive performance in Afshari ewes. Journal of Comparative Pathobiology 14: 2363-2370.
• 36. Michels H, Vanmontfort D & Dewil E (1998). Decuypere E. Genetic variation of prenatal survival in relation to ovulation rate in sheep: a review. Small Ruminant Research 29 (2): 129-142.
• 37. Mohebbi-Fani M, Mirzaei A, Nazifi S & Shabbooie Z (2012). Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages. Tropical Animal Health and Production 44: 259-265.
• 38. Murdoch WJ & Martinchict JF (2004). Oxidative damage to DNA of ovarian surface epithelial cells affected by ovulation: Carcinogenic implication and chemoprevention. Experimental Biology and Medicine 229 (6): 546-552.
• 39. Nancarrow CD (1994). Embryonic mortality in the ewe and doe, In: Zavy, M.T., Geisart, R.D. (Eds.), Embryonic Mortality in Domestic Species. London: CRC Press; pp. 79-97.
• 40. Nephew KP, Cardenas H, McClure KE, Ott TL, & Bazer F (1994). Effects of administration of human chorionic gonadotropin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. Journal of Animal Science 1994; 72: 453-458.
• 41. Özpınar H, Şenel HS, Özpınar A & Çekgül E (1994). Pharmacokinetics of intramuscular administered β-carotene and its effects on reproduction in sheep. Wiener Tierärztliche Monatsschrift 82: 229-231.
• 42. Panousis N, Giadinis N, Roubies, N, Fytianou A, Kalaitzakis, E, Pourliotis, K, Polizopoulou Z & Karatzias H (2007). Selenium, vitamin E and vitamin A status in dairy sheep reared under different feeding systems in Greece. Journal of Veterinary Medicine Series A, 54(3), 123-127.
• 43. Rapaport R, Sklan D, Wolfenson D, Shaham-Albalancy A & Hanukoglu I (1998). Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine estrous cycle. Biochimica et Biophysica Acta 1380 (1): 133-140.
• 44. Salem AA, El-Shahawy NA & Soliman IA (2015). "Effect of beta-carotene injection on estrus, vitamin A and estradiol-17-β concentrations in pubertal Farafra ewe lambs. Egypt Journal Animal Production 52 123-128.
• 45. Sreenan J, Diskin M & Dunne L (1996). Embryonic mortality: the majorcause of reproductive wastage in cattle. In: Proceedings of the 47thAnnual Meeting of the European Association of Animal Production, Lillihammer, August. Páginas.
• 46. Thatcher WW, Meyer MD & Danet-Desnoyers G (1995). Maternal recognition of pregnancy. Journal of Reproduction and Fertility 49 (Suppl.): 15-28.
• 47. Traber MG & Atkinson J (2007). Vitamin E, antioxidant and nothing more. Free Radical Biology and Medicine 43 (1): 4-15.
• 48. Wilmut I, Sales DI & Ashworth CJ (1986). Maternal and embryonic factors associated with prenatal loss in mammals. Journal of Reproduction and Fertility 76 (2), 851-864.
• 49. Yaprak M, Emsen E, Emsen B & Macit M (2004). The influence of vitamin E supplementation during late pregnancy on lamb mortality and ewe productivity in Awassi ewes. Journal of Animal and Veterinary Advances 3: 190-193.
• 50. Yokuş B, Cakır DU, Kanay Z, Gulten T & Uysal E (2006). Effects of seasonal and physiological variations on the serum chemistry, vitamins and thyroid hormone concentrations in sheep. Journal of Veterinary Medicine 53 (6): 271-276.