Synthesis of PHEMA-Based Polymeric Particles for Gallic Acid Purification

Year 2022, Volume: 5 Issue: 1, 20 - 32, 30.06.2022

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https://doi.org/10.55198/artibilimfen.1128003

Abstract

Gallic acid is commonly found in fungi and plants, particularly in thuja, oak bark, sumac, grapes, and tea leaves. Due to its antioxidant, anticancer, anti-HIV, anti-inflammatory, antimicrobial and antifungal properties, it is widely used in the field of health, as well as in various fields such as cosmetics, food, printing and dyeing industries. Therefore, the purification of gallic acid is of great importance. In this study, VIM functionalized particles were synthesized for the purification of gallic acid. The synthesized particles were characterized by scanning electron microscopy (SEM), swelling studies and surface area measurements (Brauner-Emmet-Teller, BET). The polymerization efficiency of the particles was calculated as 94% and the swelling rate as 92.5%. The surface area of the particles was determined as 45 m2/g polymer by BET analysis and it can be clearly seen that particles have size approximately below 1 μm according to the SEM analysis. Maximum gallic acid adsorption was obtained as 54.5 mg/g particles under optimum conditions. The reusability of the particles was tested with repeated adsorption- desorption cycle and it was concluded that the particles retained approximately 94.5% of the gallic acid adsorption capacity at the end of 10 cycles.

Keywords

Phenolic acids, Gallic acid, Affinity chromatography, Polymeric particles, Purification

Gallik Asit Saflaştırılması için PHEMA Temelli Polimerik Partiküllerin Sentezlenmesi

Abstract

Gallik asit mantarlarda ve bitkilerde, özellikle mazı, meşe kabuğu, sumak, üzüm ve çay yapraklarında yaygın olarak bulunur. Sahip olduğu antioksidan, antikanser, anti-HIV, antiinflamatuar, antimikrobiyal ve antifungal özellikleri nedeniyle sağlık alanında; bunun yanı sıra kozmetik, gıda, baskı ve boyama endüstrileri gibi çeşitli alanlarda yaygın olarak kullanılmaktadır. Bu yüzden, gallik asitin saflaştırılması büyük önem taşımaktadır. Bu çalışmada, gallik asidin saflaştırılması için VIM ile fonksiyonel hale getirilmiş partiküller sentezlenmiştir. Sentezlenen partiküller, taramalı elektron mikroskobu (SEM), şişme çalışmaları ve yüzey alanı ölçümleri (Brauner-Emmet-Teller; BET) ile karakterize edilmiştir. Partiküllerin polimerizasyon verimi %94, şişme oranı ise %92.5 olarak hesaplanmıştır. SEM analizi sonuçlarına göre yaklaşık 1 μm altında boyutlara sahip olduğu açıkça görülen partiküllerin yüzey alanı BET analizi ile 45 m2/g polimer olarak belirlenmiştir. Optimum koşullar altında maksimum gallik asit adsorpsiyonu 54.5 mg/g partikül olarak elde edilmiştir. Partiküllerin yeniden kullanılabilirliği tekrarlı adsorpsiyon-desorpsiyon döngüsü ile test edilmiş ve 10 döngü sonunda partiküllerin gallik asit adsorpsiyon kapasitesini yaklaşık %94.5 oranında koruduğu sonucuna varılmıştır.

Keywords

Fenolik asitler, Gallik asit, Afinite kromatografisi, Polimerik partiküller, Saflaştırma

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