Scanning Electron Microscope and Microhardness Evaluation of GIC Sealant Application After Different Enamel Treatment Procedures

Abstract

Purpose: The aim of this in vitro study was to investigate the effect of a high fluoride containing glass ionomer sealant (GIC) material (Fuji Triage/GC Europe N.V) on the hardness of the fissure enamel and its adaptation by implementing different surface preparation techniques to increase its retention. Material and Methods: Eighty freshly extracted non-carious human molar teeth were divided into four groups: (Gp1) Air-abrasion was applied Mach 4.1 (Kreativ Inc, USA) then fissures were conditioned with 20 % polyacrylic acid (Cavity conditioner/ GC Europe N.V) and sealed with Fuji Triage; (Gp2) Er,Cr:YSGG Laser were applied (Biolase Tech., San Clemente, USA) then fissures were conditioned with 20 % polyacrylic acid and sealed with Fuji Triage; (Gp 3) 20% polyacrylic acid application and fissures sealed with Fuji Triage; (Gp4) No pretreatment. Material was directly applied (control). Teeth were left in artificial saliva for three months. Then samples were sectioned buccolingually and micro-hardness measurements were carried out using Vickers apparatus. Three samples out of each treatment group was examined in Scanning Electron Microscope at x75 and x500 magnifications. Results: Scanning Electron Microscope images illustrated better adaptation of the glass ionomer cement material to the fissures when applied after Laser treatment. Conclusion: This in vitro study showed that treating enamel with laser or air abrasion before the glass ionomer sealant application effected adhesion to enamel and GIC with higher fluoride content seemed to improve enamel hardness of the fissure enamel.

Keywords

• Glass ionomer sealant, microhardness, scanning electron microscope, laser, air-abrasion

Farklı Yüzey Hazırlama Teknikleri Kullanarak Uygulanan Cam İyonomer Esaslı Fissür Örtücünün Mine Mikrosertliğine Etkisinin ve Mineyle Bağlantısının Tarayıcılı Elektron Mikroskop Altında İncelenmesi

Abstract

Amaç: Bu çalışmanın amacı, yüksek oranda fluorid içeren cam iyonomer esaslı fissür örtücü materyalinin (Fuji Triage/GC Europe N.V) retansiyonunu arttırmak için farklı yüzey hazırlama teknikleri uygulayarak, çürüksüz mine mikrosertliğine ve adaptasyonuna etkisini in-vitro olarak incelemektir. Gereç ve yöntem: Çekilmiş 80 adet çürüksüz insan 3. büyük azı dişi dört gruba ayrıldı: (Gp1) Air-abrazyon Mach 4.1 (Kreativ Inc, ABD) + %20 poliakrilik asit (Cavity conditioner/ GC Europe N.V) + Fuji Triage; (Gp2) Er,Cr:YSGG lazer (Biolase Tech., San Clemente, USA) + %20 poliakrilik asit + Fuji Triage; (Gp3) %20 poliakrilik asit + Fuji Triage; (Gp4) Fuji Triage materyali direkt olarak uygulandı (kontrol). Dişler üç ay süre ile yapay tükürükte bekletildi. Üç ay sonunda bukko lingual yönde kesit alındı; bir yüzeyinde Vickers yöntemiyle sertlik ölçümü yapıldı. Her gruptan ikişer örnek taramalı elektron mikroskobu ile x75 ve x500 büyütme altında incelendi. Bulgular: Grupların tamamında materyale yakın mikrosertlik değerlerinin ortalaması, materyale uzak mikrosertlik değerlerinin ortalamasından istatistiksel olarak anlamlı derecede yüksek bulundu (p<0,05). Gruplar arası anlamlı bir fark tespit edilmedi. Taramalı elektron mikroskobu incelemelerine göre lazer uygulanan grupta materyal mine bağlantısının daha iyi olduğu saptandı. Sonuç: Bu in-vitro çalışma; cam iyonomer esaslı fissür örtücü uygulaması öncesi minenin lazer veya airabrazyonla pürüzlendirilmesinin materyalin mineye adezyonunu ve yüksek fluorid içeren CİS materyalinin, mine mikrosertliğini arttırdığını göstermektedir.

Keywords

• Cam iyonomer fissür örtücü, mikrosertlik, taramalı elektron mikroskobu, lazer, airabrazyon

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