ANADOLU'NUN ÜÇ FARKLI BÖLGESİNDEN ALINAN PROPOLİS ÖRNEKLERİNİN ANTİMİKROBİYAL AKTİVİTELERİNİN İN VİTRO OLARAK İNCELENMESİ

Abstract

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Keywords

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IN VITRO ANTIMICROBIAL ACTIVITY OF PROPOLIS SAMPLES FROM THREE DIFFERENT REGIONS OF ANATOLIA

Abstract

Bu çalışmanın amacı, Türkiye'nin Zara/Sivas, Trabzon ve Aydın bölgelerinden alınan propolis örneklerinin, agar difüzyon yöntemi ile antimikrobiyal aktivitelerinin incelenmesidir. Çalışmada, farklı propolis ekstratlarının (%10 ve %20) E. faecalis, S. aureus, E. coli ve C. albicans üzerine olan antimikrobiyal aktiviteleri test edildi. Mikroorganizmalar Mueller Hinton (MH) agar içeren plaklara ekildi. Daha sonra ekim yapılan agar plaklarına kuyucuklar açıldı ve test solüsyonları ile dolduruldu. MH agar plakları 37°C'de 24 saat inkübe edildi ve inhibisyon alanlarının genişlikleri ölçülerek kaydedildi. Her solüsyon için bu test 7 kere tekrarlandı. Çalışmanın sonucunda, %2,5'lik sodyum hipoklorit (NaOCl) ve tüm propolis ekstratlarının test edilen mikroorganizmalara karşı antimikrobiyal aktivitesinin olduğu görüldü. Bununla birlikte, %2,5'lik NaOCl'nin en etkili irrigan olduğu ve tüm mikroorganizmalara karşı diğer solüsyonlarla arasında istatistiksel olarak fark olduğu görüldü (p

Keywords

• Antimikrobiyal aktivite, NaOCl, propolis

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