ANADOLU'NUN ÜÇ FARKLI BÖLGESİNDEN ALINAN PROPOLİS ÖRNEKLERİNİN ANTİMİKROBİYAL AKTİVİTELERİNİN İN VİTRO OLARAK İNCELENMESİ

Öz

-

Anahtar Kelimeler

• -

IN VITRO ANTIMICROBIAL ACTIVITY OF PROPOLIS SAMPLES FROM THREE DIFFERENT REGIONS OF ANATOLIA

Öz

Bu çalışmanın amacı, Türkiye'nin Zara/Sivas, Trabzon ve Aydın bölgelerinden alınan propolis örneklerinin, agar difüzyon yöntemi ile antimikrobiyal aktivitelerinin incelenmesidir. Çalışmada, farklı propolis ekstratlarının (%10 ve %20) E. faecalis, S. aureus, E. coli ve C. albicans üzerine olan antimikrobiyal aktiviteleri test edildi. Mikroorganizmalar Mueller Hinton (MH) agar içeren plaklara ekildi. Daha sonra ekim yapılan agar plaklarına kuyucuklar açıldı ve test solüsyonları ile dolduruldu. MH agar plakları 37°C'de 24 saat inkübe edildi ve inhibisyon alanlarının genişlikleri ölçülerek kaydedildi. Her solüsyon için bu test 7 kere tekrarlandı. Çalışmanın sonucunda, %2,5'lik sodyum hipoklorit (NaOCl) ve tüm propolis ekstratlarının test edilen mikroorganizmalara karşı antimikrobiyal aktivitesinin olduğu görüldü. Bununla birlikte, %2,5'lik NaOCl'nin en etkili irrigan olduğu ve tüm mikroorganizmalara karşı diğer solüsyonlarla arasında istatistiksel olarak fark olduğu görüldü (p

Anahtar Kelimeler

• Antimikrobiyal aktivite, NaOCl, propolis

Kaynakça

1. Sundqvist GK, Eckerbom MI, Larsson AP, Sjögren UT. Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections. Infect Immun 1979; 25: 685-93.
2. Baumgartner JC, Watkins BJ, Bae KS, Xia T. Association of black-pigmented bacteria with endodontic infections. J Endod 1999; 25: 413-5.
3. Sundqvist GK, Johansson E, Sjögren UT. Prevalence of black-pigmented bacteroides species in root canal infections. J Endod 1989;15: 13-9.
4. Moller AJR, Fabricius L, Dahlen G, Ohman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res 1981; 89: 475-84.
5. Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontits. Int Endod J 1998; 31: 1-7.
6. Walton RE, Torabinejad M. Principles and practices of endodontics. 2nd ed. Philadelphia (PA): B. Saunders; 1996.
7. Harrison JW, Hand RE. The effect of dilution and organic matter on the antibacterial property of 5.25% sodium hypochlorite. J Endod 1981; 7:128-32.
8. Izu KH, Thomas SJ, Zhang P, Izu AE, Michalek S. Effectiveness of sodium hypochlorite in preventing inoculation of periapical tissues with contaminated patency files. J Endod 2004; 30:92-4.

9. Sabala CL, Powell SE. Sodium hypochlorite injection into periapical tissues. J Endod 1989;15: 490-2.
10. Becking AG. Complications in the use of sodium hypochlorite during endodontic treatment. Oral Surg Oral Med Oral Pathol 1991; 72: 346-8.
11. Ozturk B, Ozer F. Effect of NaOCl on bond strengths of bonding agents to pulp chamber lateral walls. J Endod 2004; 30: 362-5.
12. Uzel A, Sorkun K, Önçag Ö, Çogulu D, GenÇay Ö, Salih B. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res 2005; 160: 189–95.
13. Gopikrishna V, Baweja PS, Venkateshbabu N, Kandaswamy D. Comparison of coconut water, propolis, HBSS, and milk on PDL cell survival. J Endod 2008; 34: 587–9.
14. Basrani B, Tjäderhane L, Santos JM, Pascon E, Grad H, Lawrence HP, Friedman S. Efficacy of chlorhexidine- and calcium hydroxide-containing medicaments against Enterococcus faecalis in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96: 618-24.
15. Jeansonne MJ, White RR. A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants. J Endod 1994; 20: 276–8.
16. Ayhan H, Sultan N, Çirak M, Ruhi MZ, Bodur H. Antimicrobial effects of various endodontic irrigants on selected microorganisms. Int Endod J 1999; 32: 99–102.
17. Serper A, Ozbek M, Calt S. Accidental sodium hypochlorite induced skin injury during endodontic treatment. J Endod 2004; 30: 180-181.
18. Keçeci AD, Üreyen Kaya B, Çelik Ünal G. Inadverdent Injection of Sodium Hypochlorite Into Periapical Tissues: Two Case Reports. H Ü Diş Hek Fak Derg 2006; 30: 35-41.
19. Al-Shaher A, Wallace J, Agarwal S, Bretz W, Baugh D. Effect of propolis on human fibroblasts from the pulp and periodontal ligament. J Endod 2004; 30: 359-61.
20. Park YK, Koo MH, Abreu JA, Ikegaki M, Cury JA, Rosalen PL. Antimicrobial activity of propolis on oral microorganisms. Curr Microbiol 1998; 36:24–8.
21. Magro-Filho O, Carvalho AC. Topical effect of propolis in the repair of sulcoplasties by the modified Kazanjian technique. J Nihon Univ Sch Dent 1994; 36: 102–11.
22. Toker H, Ozan F, Ozer H, Ozdemir H, Eren K, Yeler H. A morphometric and histopathologic evaluation of the effects of propolis on alveolar bone loss in experimental periodontitis in rats. J Periodontol 2008; 79: 1089-94.
23. Marcucci MC. Propolis: chemical composition, biological properties and therapeutic activity. Apidologie 1995; 26: 83-99.
24. Cook NC, Samma S. Flavonoids: chemistry, metabolism, cardioprotective effects and dietary sourses. Nutr Biochem 1996; 7: 66-76.
25. Mirzoeva OK, Grishanin RN, Calder PC. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol Res 1997; 152: 239-46.
26. Amaros M, Lurton E, Boustie J, Girre L, Sauvager F, Cormier M. Comparison of the anti-herpes simplex virus activities of propolis and 3-methylbut-2-enyl caffeate. J Nat Prod 1994; 57: 644-7.
27. Bonhevi JS, Coll FV, Jrda RE. The composition, active components and bacteriostatic activity of propolis in dietetics. J Am Oil Chem Soc 1994; 71: 529-32.
28. Koo H, Gomes BP, Rosalen PL, Ambrosano GM, Park YK, Cury JA. In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Arch Oral Biol 2000; 45: 141-8.
29. Ozan F, Sümer Z, Polat ZA, Er K, Ozan U, Deḡer O. Effect of Mouthrinse Containing Propolis on Oral Microorganisms and Human Gingival Fibroblasts. Eur J Dent 2007; 1: 195-201.
30. Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol 1999; 64: 235-40.
31. Ferreira FB, Torres SA, Rosa OP, Ferreira CM, Garcia RB, Marcucci MC, Gomes BP. Antimicrobial effect of propolis and other substances against selected endodontic pathogens. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 104:709-16.
32. Oncag O, Cogulu D, Uzel A, Sorkun K. Efficacy of propolis as an intracanal medicament against Enterococcus faecalis. Gen Dent 2006; 54: 319-22.