Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats

Royal Shirinov; İsmet Burcu Türkyılmaz; Eda Dağsuyu; Refiye Yanardağ

doi:10.33988/auvfd.1731146

Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats

Abstract

Valproic acid (VPA) is a short-chain fatty acid used to treat disorders of the brain. When VPA is used for long term, it causes damage, particularly through the formation of free radicals. The lungs are among the organs most affected by long-term VPA treatment. Some antioxidant substances are used to prevent damage caused by free radicals. One of these substances is alpha-lipoic acid (ALA), which occurs naturally. In our study, the protective effect of ALA against VPA-induced lung injury was investigated. In this study, female Sprague-Dawley rats were divided into four groups. The 1st group served as the control (1 mL olive oil); the 2nd group received ALA (50 mg/kg/day) for 15 days; the 3rd group received VPA (0.5 g/kg/day) for 15 days; and the 4th group received both VPA and ALA at the same doses and for the same duration. ALA and VPA were dissolved in olive oil. On day 16, all groups were euthanized under anesthesia. The lung tissues were collected and homogenized. In the prepared supernatants, the levels of reduced glutathione and total antioxidant status, and the activities of glutathione reductase, glutathione peroxidase, catalase, paraoxonase, carbonic anhydrase, aryl esterase, and superoxide dismutase were decreased in the VPA group, while sialic acid, lipid peroxidation, reactive oxygen species, total oxidant, oxidative stress index, hydroxyproline, glycoprotein levels, and xanthine oxidase activities were increased in the VPA group. These values were reversed in ALA when VPA was administered. As a result, it may be concluded that ALA exerts protective effects against VPA-induced lung damage.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Biochemistry

Journal Section

Research Article

Authors

Royal Shirinov This is me
0009-0003-0620-8400
Türkiye

İsmet Burcu Türkyılmaz
0000-0003-2789-5943
Türkiye

Eda Dağsuyu
0000-0003-0395-1058
Türkiye

Refiye Yanardağ ^*
0000-0003-4185-4363
Türkiye

Early Pub Date

January 22, 2026

Publication Date

January 22, 2026

Submission Date

June 30, 2025

Acceptance Date

December 6, 2025

Published in Issue

Year 2026 Volume: 73 Number: 2

DOI

https://doi.org/10.33988/auvfd.1731146

IZ

https://izlik.org/JA69FG95RS

Cite

RIS / Bibtex

APA

Shirinov, R., Türkyılmaz, İ. B., Dağsuyu, E., & Yanardağ, R. (2026). Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 73(2), 177-184. https://doi.org/10.33988/auvfd.1731146

AMA

1.Shirinov R, Türkyılmaz İB, Dağsuyu E, Yanardağ R. Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats. Ankara Univ Vet Fak Derg. 2026;73(2):177-184. doi:10.33988/auvfd.1731146

Chicago

Shirinov, Royal, İsmet Burcu Türkyılmaz, Eda Dağsuyu, and Refiye Yanardağ. 2026. “Protective Effect of Alpha Lipoic Acid Against Oxidative Damage Caused by Valproic Acid on Lung Tissue in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 (2): 177-84. https://doi.org/10.33988/auvfd.1731146.

EndNote

Shirinov R, Türkyılmaz İB, Dağsuyu E, Yanardağ R (April 1, 2026) Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 2 177–184.

IEEE

[1]R. Shirinov, İ. B. Türkyılmaz, E. Dağsuyu, and R. Yanardağ, “Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats”, Ankara Univ Vet Fak Derg, vol. 73, no. 2, pp. 177–184, Apr. 2026, doi: 10.33988/auvfd.1731146.

ISNAD

Shirinov, Royal - Türkyılmaz, İsmet Burcu - Dağsuyu, Eda - Yanardağ, Refiye. “Protective Effect of Alpha Lipoic Acid Against Oxidative Damage Caused by Valproic Acid on Lung Tissue in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73/2 (April 1, 2026): 177-184. https://doi.org/10.33988/auvfd.1731146.

JAMA

1.Shirinov R, Türkyılmaz İB, Dağsuyu E, Yanardağ R. Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats. Ankara Univ Vet Fak Derg. 2026;73:177–184.

MLA

Shirinov, Royal, et al. “Protective Effect of Alpha Lipoic Acid Against Oxidative Damage Caused by Valproic Acid on Lung Tissue in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 73, no. 2, Apr. 2026, pp. 177-84, doi:10.33988/auvfd.1731146.

Vancouver

1.Royal Shirinov, İsmet Burcu Türkyılmaz, Eda Dağsuyu, Refiye Yanardağ. Protective effect of alpha lipoic acid against oxidative damage caused by valproic acid on lung tissue in rats. Ankara Univ Vet Fak Derg. 2026 Apr. 1;73(2):177-84. doi:10.33988/auvfd.1731146