Evaluation of oxidative stress, antioxidant status and lipid profile in cattle with displacement of the abomasum

Abstract

The aims of the present study were to evaluate serum oxidative and antioxidant status and oxidative stress index, and lipid metabolism profile in cattle with abomasal displacement. A total of thirty-two dairy cows with right- and left-sided abomasal displacement (LDA (n=11) and RDA without volvulus (n=11)) and 10 healthy controls were used in this study. Serum total oxidative status (TOS) and total antioxidant capacity (TAC) were measured by a colorimetric method. The TOS-to-TAC ratio was also calculated as oxidative stress index (OSI) value. Serum lipid profiles were determined by conventional measurement methods as well. Mean TOS and OSI values were significantly (p<0.001) higher in cows with RDA compared to LDA and healthy controls; however, there was no significant differences in serum TAC values among groups. Serum total cholesterol (TC), high density lipoproteins (HDL) and low density lipoproteins (LDL) levels were significantly (p=0.001) decreased, while serum triglyceride (TG) levels were significantly increased in dairy cows with right- and left-sided abomasal displacement compared to healthy controls. The results of current study showed that dairy cows with RDA forming free radicals than LDA and controls due to abomasal tension and increased intraluminal pressure

Keywords

• Dairy cow
• displacement abomasum
• oxidative stress

Abomazum deplasmanlı sığırlarda oxidatif stres, antioksidatif seviye ve lipit profilinindeğerlendirilmesi

Abstract

Bu çalışmanın amacı abomazum deplasmanlı sığırlarda serum oksidan ve antioksidant seviyeleri ve okidatif stres indeksini (OSI) değerlendirmektir. Bu çalışmada toplam sağa ve sola abomasum deplasmanı olan 32 (11 sola deplasman, 11 volvulus olmayan sağa deplasman) ve 10 sağlıklı Holstein sığır kullanıldı. Serum toplam oksidan seviye (TOS) ve toplam antioksidan kapasite (TAC) kolarimetrik bir yöntemle belirlendi. OSI değeri, TAC-TOS oranı hesaplanarak belirlendi. Serum lipid profili ise geleneksel yöntemlerle belirlendi. TOS ve OSI değerleri, sola deplasmanlı sığırlar ve kontrol grubuyla karşılaştırıldığında sağa deplasmanlı sığırlarda önemli oranda (P<0.001) yüksek bulundu. Fakat serum TAC değerlerinde gruplar arasında önemli bir fark yoktu. Sağa ve sola deplasmanı olan sütçü sığırlar kontrolle karşılaştırıldığında serum trigliserit (TG) sevisinde önemli bir artış belirlenirken, serum total kolestrol (TC), yüksek dansiteli lipoproteinler (HDL) ve düşük dansiteli lipoprotein (LDL) seviyelerinde önemli bir azalma belirlendi. Bu çalışmanın sonuçlarında, RDA’lı sütçü sığırlar da intraluminal basınç artışı ve abomazumdaki gerginlikten dolayı LDA ve kontrollerden daha fazla serbest radikaller oluştuğu gösterildi

Keywords

• Abomasum deplasmanı
• oksidatif stress
• sütçü sığır

References

1. 1. Bernabucci U, Ronchi B, Lacetera N, et al. (2005): Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science, 88, 2017-2026.
2. 2. Bobe G, Young JW, Beitz DC (2004): Invited Review: Pathology, etiology, prevention, and treatment of fatty liver in dairy cows. Journal of Dairy Science, 87, 3105- 3124.
3. 3. Cameron REB, Dyk PB, Herdt TH, et al. (1998): Dry cow diet, management, and energy balance as risk factors for displaced abomasum in high producing dairy herds. Journal of Dairy Science, 81, 132-139.
4. 4. Celi P (2011a): Oxidative stress in ruminants. In: oxidative stress in applied basic research and clinical practice (Ed. D. Armstrong). Humana Press, pp.191-230.
5. 5. Celi P (2011b): Biomarkers of oxidative stress in ruminant medicine. Immunopharmacology Immunotoxicology, 332, 233-240.
6. 6. Constable PD, Miller GY, Hoffsis GF, et al. (1992): Risk factors for abomasal volvulus and left abomasal displacement in cattle. American Journal Veterinary Research, 53, 1184-1192.
7. 7. Durgut R, Ataseven VS, Ozturk AS, et al. (2013): Evaluation of total oxidative stress and total antioxidant status in cows with natural bovine herpesvirus-1 infection. Journal of Animal Science, 91, 3408-3412.
8. 8. Erel O (2004): A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry, 37, 112-119.

9. 9. Erel O (2005): A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38, 1103-1111.
10. 10. Furll M, Dabbagh MN, Kirbach H, et al. (2003): The anti-oxidative state before and after a diagnosed abomasal displacement in cows. Acta Veterinaria Scandinavica, 44, 37.
11. 11. Gaal T, Ribiczeyne-Szabo P, Stadler K, et al. (2006): Free radicals, lipid peroxidation and the antioxidant system in blood of cows and newborn calves around calving. Comparative Biochemical Physiology Part B, 143, 391-396.
12. 12. Geishauser T (1995): Abomasal displacement in the bovine—a review on character, occurrence, aetiology and pathogenesis. Zentralbl Veterinaermed A, 42, 229-251.
13. 13. Gerloff BJ, Herdt TH, Emery RS (1986): Relationship of hepatic lipidosis to health and performance in dairy cattle. Journal American Veteterinary Medical Associotion, 188, 845-850.
14. 14. Harma M, Harma M, Erel O (2005): Measurement of the total antioxidant response in preeclampsia with a novel automated method. European Journal of Obstetrics Gynecology Reproductive Biology, 118, 47-51.
15. 15. Hasanpour A, Saranjam N, Amuoghli Tabrizi B (2011): Antioxidant concentration status in the serum of cows with left displacement abomasom. Global Veterinaria, 7, 478- 481.
16. 16. Itoh N, Koiwa M, Hatsugaya A, et al. (1998): Comparative analysis of blood chemical values in primary ketosis and abomasal displacement in cows. Journal of Veterinary Medicine Serie A, 45, 293-298.
17. 17. Lykkesfeldt J, Svendsen O (2007): Oxidants and antioxidants in disease: oxidative stress in farm animals. Veterinary Journal, 173, 502-511.
18. 18. Maden M, Ozturk SA, Bulbul A, et al. (2012): Acute phase proteins, oxidative stress and enzyme activities of blood serum and peritoneal fluid in abomasal displacement cases. Journal of Veterinary Internal Medicine, 26, 1470-1475.
19. 19. Mudron P, Rehage J, Sallmann HP, et al. (1997): Plasma and liver alpha-tocopherol in dairy cows with left abomasal displacement and fatty liver. Zentralbl Veterinarmed. A, 44, 91-97.
20. 20. Oliveira CP, Da Costa Gayotto LC, Tatai C, et al. (2005): Oxidative stress in the pathogenesis of nonalcoholic fatty liver disease, in rats fed with a cholinedeficient diet. Journal of Cellular and Moleculer Medicine, 6, 399-406.
21. 21. Oliveira CP, Coelho AM, Barbeiro HV, et al. (2006): Liver mitochondrial dysfunction and oxidative stress in the pathogenesis of experimental nonalcoholic fatty liver disease. Brazilian Journal of Medical and Biological Research, 39, 189-194.
22. 22. Qu Y, Lytle K, Traber MG, et al. (2013): Depleted serum vitamin E concentrations precede left displaced abomasum in early-lactation dairy cows. Journal Dairy Science, 96, 3012-3022.
23. 23. Radostits OM, Gay CC, Hinchliff K, et al. (2007): Veterinary Medicine. A textbook of the diseases of cattle, horse, sheep, pigs and goats.10t h. Ed., Elsevier Publisher, Amstrerdam.
24. 24. Rayssiguier Y, Mazur A, Gueux E, et al. (1998): Plasma lipoproteins and fatty liver in dairy cows. Research in Veterinary Science, 45, 389-393.
25. 25. Sattler T, Fuerll M (2002): Antioxidative status (SOD, GPX) of cows with displaced abomasum, clinical significance and influence of the treatment with antioxidants. XXII. World Buiatrics Congress Proocedings, Hannover, p:397-456.
26. 26. Sordillo LM, O’boyle N, Gandy JC, et al. (2007): Shifts in thioredoxin reductaseactivity and oxidant status in mononuclear cells obtained from transition dairy cattle. Journal of Dairy Science, 90, 1186-1192.
27. 27. Van Winden S (2002): Displacement of the abomasum in dairy cows -risk factors and pre-clinical alterations. Dissertation Utrecht University. Faculty of Veterinary Medicine. ISBN90-393-3180-4. pp:99.