Procalcitonine, Nitric oxide and C-Reactive Protein concentrations in rats with experimentally-induced infectious and noninfectious inflammation

Abstract

The aim of this study is to determine the alterations in procalcitonine (PCT), nitric oxide (NO) and C-reactive protein (CRP) levels in infectious and noninfectious inflammation and to evaluate their potential in the differentiation of inflammation. Totally 70 female Wistar rats (Group 1: n=21; Group 2, n=21, Group 3: n=21; Day 0: n=7), weighing 250-300 grams, were used in this study. Before the applications, blood samples were collected in seven rats and the time is accepted as Day 0. Three groups were formed each containing 21 rats. Stapylococcus aureus were administered at a concentration of 10 6 CFU in the infection group (Group 1), subcutaneously. Turbentine oil at a dose of 0.5 mg/kg were injected subcutaneously to twenty-one rats in the noninfectious inflammation group (Group 2). In the control group, 21 rats were injected with saline subcutaneously. Blood samples were collected from randomly selected 7 rats in each group on the 1st, 4th and the 7th days. PCT, NO, and CRP analyzes were performed with commercial test kits in the ELISA reader. According to the obtained results, we determined that serum PCT, NO and CRP levels increased after administrations. The rises of CRP and PCT concentrations were not statistically significant but it was higher in infectious group levels than the other two groups. Based on these results, it can be said that NO shows significant increases during infectious inflammation and can be used for diferential diagnostic purposes in clinics

Keywords

• CRP
• inflammation
• NO
• PCT
• rat

Deneysel Olarak İnfeksiyöz ve Noninfeksiyöz Yangı Oluşturulmuş Ratlarda Prokalsitonin, NitrikOksit ve C-Reaktif Protein Konsantrasyonları

Abstract

Bu çalışmanın amacı, infeksiyöz ve noninfeksiyöz yangı durumunda prokalsitonin(PCT), Nitrik oksit (NO) ve C-reaktif protein (CRP) düzeylerini belirlemek ve yangının ayrımının yapılmasında kullanılabilirliğini değerlendirmektir. Bu çalışmada toplam olarak 250-300 gram ağırlığında 70 dişi Wistar rat (Grup 1: n=21, Grup 2: n=21, Grup 3: n=21 ve 0. Gün n=7) kullanıldı. Uygulamalardan önce 7 rattan kan örnekleri alınıp 0. gün olarak kabul edildi. Ayrıca her biri 21 rat içeren 3 grup oluşturuldu. İnfeksiyon grubunda (Grup 1) bulunan 21 rata Staphylococcus aureus 106 CFU derialtı olarak verildi. Noninfeksiyöz yangı grubunda bulunan 21 rata Turbentin yağı 0.5 mg/kg derialtı uygulandı. Kontrol grubundaki 21 rata derialtı fizyolojik tuzlu su verildi. Uygulamalardan sonra her gruptan rastgele seçilen yedişer hayvandan, 1., 4. ve 7. günlerde kan örnekleri toplandı. PCT, CRP ve NO düzeyleri ELISA cihazında ticari test kitleriyle çalışıldı. Elde edilen sonuçlara göre PCT, NO ve CRP düzeylerinin uygulamalardan sonra arttığını belirlendi. CRP ve PCT düzeylerindeki artış infeksiyöz grupta diğer iki gruba göre daha fazlaydı ancak istatistiki önem saptanamadı. Bu çalışmanın sonuçlarına dayanarak, NO’in infeksiyöz yangıda önemli düzeyde arttığı ve kliniklerde ayırıcı tanı amaçlı kullanılabileceği söylenebilir

Keywords

• CRP
• PCT
• rat
• NO
• yangı

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