A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats

Abstract

This study was performed to specify the distribution and the heterogeneity of mast cells found in lungs and trachea of Van Cats. Four Van Cats were used in the study. Appropriate sized pieces were taken from the lungs and tracheas of the cats. After BLA (Basic Lead Acetate-Mota) was set, it was blocked with paraplast by doing the routine tissue follow-up. Sections taken in 6-7 μm thickness were stained with 0,5 % toluidine blue and alcian-blue safranin O combined stain. Numbers of mast cells were specified semi-quantitatively in the sections stained with toluidine blue under lens 40. Among analysed organs, the most mast cells were found in lungs. To specify the granule construction, BLA detection was determined to be suitable. In the stain of Alcianblue safranin O, SO (+), AB (+) granulose mast cells were found; mast cells that have mixed granules were not found

Keywords

• Heterogeneity
• light microscope
• lung
• mast cell
• trachea
• Van Cats

Van Kedilerinin trakeya ve akciğerlerinde bulunan mast hücrelerinin dağılımı ve heterojenitesiüzerine morfolojik ve histometrik çalışma

Abstract

Bu çalışma Van Kedilerinde trakeya ve akciğerlerde bulunan mast hücrelerinin dağılımı ve heterojenitesini belirlemek amacıyla yapıldı. Çalışmada 4 adet Van Kedisi kullanıldı. Van Kedilerinin trakeya ve akciğerlerinden uygun büyüklükte parçalar alındı. BLA (Basic Kurşun Asetat-Mota)’da tespit edildikten sonra rutin doku takibi yapılarak paraplast ile bloklandı. 6-7 µm kalınlığında alınan kesitler % 0,5’lik toluidine blue ve alcian blue-safranin O kombine boyalarında boyandılar. Toluidine blue ile boyanan kesitlerde 40’lık objektifte mast hücre sayıları semikantitatif olarak belirlendi. İncelenen organlardan akciğerde en fazla sayıda mast hücresine rastlandı. Granül yapısını belirlemede BLA tespitinin uygun olduğu saptandı. Alcian blue- safranin O boyamasında ise trakeya ve akciğerde SO (+), AB (+) granüllü mast hücrelerine rastlandı, miks granüle sahip mast hücrelerine ise rastlanılamadı

Keywords

• Akciğer
• heterojenite
• ışık mikroskobu
• mast hücresi
• trakeya
• Van Kedisi

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