INVESTlGATlONS ON IN VlTRO FERTlLlZATlON OF RABBIT OVA

Abstract

In this study, in vitro fertilization of rabbit ova was investigated. For this purpose, 42 females and 6 male rabbits (New Zealand) were used during the research. The experimental animals were divided into 12 groups. PMSG (150-250 LU) and HCG (75-100 LU) were injected for superfolliculation and superovulation. Totally 62 follicles and 616 ovulation places (corpus haemorrhagicum) were counted in different times following administration of gonadotrophins. Two hundredfiftyone (37.02 %) ova were collected by flushing oviducts and ruptured follicles with the defined medium. Collected ova were identi/ied under stereo microscope and estimated as normal and degenerated. One Iıundred fourty five (57.76 %), 102 (40.63 %), and 4(1.59 %) of ova were covered with zona pellucida, corona radiata and cumulus oophorus, respectively. Totally 236 normalova were used for in vitro fertilization. These normalova were incubated witlı capacitated spermatozoa in tlıe in vitro fertilization medium under paraffin oif whiclı had been equilibrated with 5 % COı + 95 % Nı at 37-38 oC in a incubator. Af ter 24-28 hours incubation, the ova were controlled under stereo microscope and were classified as fertilized or unfertilized According to the results, tlıe in vitro fertilization was observed in 47 (19.91 %) out of 236 ova and 34 of fertifized ova were normal and the rest (13 ova) were abnormal. One hundred eightynine ova were foul/d as unfertilized and degenerated.

Keywords

• INVESTlGATlONS
• FERTlLlZATlON
• RABBIT OVA

References

1. Ankara Üniversitesi – Ankara Üniversitesi Veteriner Fakültesi Dergisi