Cymbopogon spp. ve Helianthemum spp. Bitki Ekstraktlarının In-Vitro Antibakteriyal Aktivitelerinin Araştırılması

Yıl 2019, Cilt: 2 Sayı: 3, 131 - 136, 30.09.2019

Mustafa Tokar

Öz

Patojen mikroorganizmalara karşı kullanılan antibakteriyel maddelere direncin artması ve gıda katkı maddelerinin istenmeyen yan etkileri nedeniyle, son yıllarda yeni bitki kaynaklı antibakteriyel madde arayışını arttırmıştır. Bu çalışmada, Türkiye’den toplanan Cymbopogon jwarancusa, Hindistan’dan toplanan Cymbopogon citratus ve Güney Afrika’dan toplanan Cymbopogon nardus türleri ile Mısır’dan toplanan Helianthemum kahiricum ve Suriye’den toplanan Helianthemum lippi türü bitki ekstraktlarının hastane enfeksiyonlarının etiyolojisinde yer alan Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aureginosa patojen türleri ile enteropatojenlerden; Salmonella typhi, Escherichia coli, türlerinin antibakteriyel aktivitelerinin disk difüzyon yöntemiyle araştırılması amaçlandı. Disk diffüzyon sonuçlarına göre, yalnızca E. coli ve S. aureus’a karşı tüm bitki ekstraktlarının (20μg/disk) farklı düzeylerde zon çapı oluşturduğu (16-38 mm) belirlendi. Bitki türleri içerisinde en yüksek antibakteriyel aktivite C. citratus ekstraktında tespit edildi. Sonuç olarak, başta C. citratus türü ekstraktı olmak üzere, araştırılan bitki ekstraktlarının sağlık, farmasötik, kozmetik ve gıda endüstrisi gibi birçok alanda mikroorganizmalara karşı etkili olduğu ve bu konuda daha ileri düzeyde in vitro ve in vivo çalışmaların yapılması gerektiği düşünülmektedir.

Anahtar Kelimeler

Disk difüzyon, Antibakteriyel aktivite, Hastane enfeksiyonu

Destekleyen Kurum

Çankırı Karatekin Üniversitesi BAP birimi

Proje Numarası

SYO060515B24 nolu proje

Teşekkür

Projeye katkılarından dolayı Türkiye Halk Sağlığı Kurumu Başkanlığı ve Ankara Üniversitesi Ziraat Fakültesi Bitki Koruma Bölüm Başkanlığı’ na teşekkür ederiz.

Investigation of Antibacterial Activities of Plant Extracts Prapered from Cymbopogon ssp. and Helianthemum ssp.

Öz

New plant-derived antibacterial agent researches have increased in recent years, because of increased resistance to antimicrobial agents that used against to pathogen microorganisms, and unwanted side effects of food additives. The aim of this research was to investigate the, in-vitro antibacterial activities and collected from Turkey Cymbopogon jwarancusa on, collected from India Cymbopogon citratus and collected from Egypt with Cymbopogon nardus species collected from South Africa Helianthemum kahiric and collected from Syria Helianthemum lippi species of plants extracts on as with pathogens species; Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aureginosa and as from entheropathogens Salmonella typhi, Escherichia coli, of which also in the etiology of nosocomial infections, by disk diffusion method.The diameter of the inhibition zone in the disc-diffusion method shows that all the plant extracts (20μg/disc) have antibacterial activity only on E.coli and S.aureus different levels (16-38 mm). C. citratus extract showed the highest antibacterial activity with disc diffusion method. In conclusion, particularly C.citratus plant extract, besides other plant extracts are thought to be effective against to microorganisms in many areas like medical, cosmetic and food industries. But there is need new in vitro and in vivo studies in future.

Anahtar Kelimeler

Antibacterial activity, Disc diffusion, Nosocomial

Proje Numarası

SYO060515B24 nolu proje

Kaynakça

• Abd-El Fattah SM, Hassan ASY, Bayoum HM, Eissa HA. (2010). The use of lemongrass extracts as antimicrobial and food additive potential in yoghurt. J. Am. Sci, 6: 582-594.
• Almagboul AZ, Bashir AK, Farouk A, Salih AKM. (1985). Antimicrobial activity of certain Sudanese plants used in folkloric medicine. Screening for antibacterial activity. Fitoterapia, 56: 331-337.
• Aşkar Ş, Deveboynu ŞN. (2018). Investigation of In-Vitro Antibacterial Activity of Curcuma longa Commercial Extract. Eurasian JHS, 1(1):1-6.
• Bağcı E, Dığrak M. (1996). Bazı orman ağaçlarının uçucu yağlarının antimikrobiyal aktiviteleri. Tr. J. of Biology, 20: 191-198.
• Barbosa E, Calzada F, Campos R. (2006). Antigiardial activity of methanolic extracts from Helianthemum glomeratum Lag. and Rubus coriifolius Focke in suckling mice CD-1. Journal of Ethnopharmacology, 108:395–397.
• Berlin B, Berlin E. (1995). Medical Ethnobiology of the Highland of Chiapas, Mexico. The Gastrointestinal Diseases, Princeton University Press, New Jersey.
• Billerbeck VG, Roques CG, Bessiere JM, Fonvieille JL, Dargent R. (2001). Effect of Cymbopogon nardus (L.) W.Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian J Microb, 47: 9-17.
• Bradshaw JS, Krzysztof E, Krakowiak R, Izatt, M.(1992). The Chemistry of Heterocyclic Compounds. Aza-Crown Macrocycles,Wiley Publication.
• Bouriche H, Kada S, Assaf AM, Senatör A, Gül F, Demirtaş I. (2016). Phytochemical screening and anti-inflammatory properties of Algerian Hertia cheirifolia methanol extract Journal Pharmaceutical Biology, 54 (11): 2584–2590.
• Carlini EA, Contar JDP, Silva-Filho AR. (1968). Pharmacology of lemongrass (Cymbopogon citratus Stapf). I. Effects of teasprepared from the leaves on laboratory animals. J Ethnopharmacol, 17:37-64.
• Chandan Prasad et al. (2011).Antioxidant and antimicrobial activity of ethanol and water extracts of Cymbopogonm jwarancusa (Jones.) leaves. Journal of Applied Pharmaceutical Science 01 (09) : 68-72.
• Ekpenyong CE, AkpanEE. (2017). Use of Cymbopogon citratus essential oil in food preservation: Recent advances and future perspectives. Critical Reviews in Food Science and Nutrition, 57(12): 2541–2559.
• Ermeli NB et al. (2012). Screening of analgesic and antiinflammatory activities for two Libyan medicinal plants:Helianthemum lippii and Launaea residifolia. Journal of Chemical and Pharmaceutical Research, 4(9):4201-4205.
• Ewansiha JU, Garba SA, Mawak JD, Oyewole OA. (2012). Antimicrobial Activity of Cymbopogon Citratus (Lemon Grass) and its Phytochemical Properties. Frontiers in Science, 2(6): 214-220.
• Helal GA, Sarhan MM, Abu Shahla ANK, El-Khai EKA. (2006). Antimicrobial activity of some essential oils against microorganisms deteriorating fruit juices. Mycobiol, 34: 219-229.
• Javidnia K, Nasiri A, Miri R, Jamalian A. (2007). Compositon of Essential oil Teucrium persicum Boiss.from Iran. J.Essential Oil Res, 19: 430-432.
• Meckes M, Villareal ML, Tortoriello J, Berlin B, Berlin E. (1995). A microbiological evaluation of medicinal plants used by Maya people of Southern Mexico, Phytother.Res, 9:244-250.
• Moraga AB et al. (2013). Screening for polyphenols, antioxidant and antimicrobial activities of extracts from eleven Helianthemum taxa (Cistaceae) used in folk medicine in south-eastern Spain. Journal of Ethnopharmacology, 148:287–296.
• Nostro AM, Germano VD, Ángelo and Cannatelli M. (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microbiol, 30:379-384.
• Pratt DE, Hudson BJF. (1991). Antioxidant activity of phenolic compounds in meat model systems. In phenol compounds in food and their effects on health. AC Symposium Serieus 506; American Chemical Society, 1990.Washington, 214-222.
• Saxena G, McCutcheon AR, Farmer S, Towers GHN, Hancock REW.(1994). Antimicrobial constituents of Rhus glabra. J. Ethnopharmacol, 42: 95-99.
• Shadab Q, Hanif M, Chaudhary FM.(1992). Antifungal activity by lemongrass essential oils. Pak. J. Sci. Ind. Res, 35: 246-249.
• Sarath Chandra Bose N Ammani K and Ratakumari S.(2013). Chemical Composition and Its Antibacterial Activity of Essential Oil from Cymbopoton Jwarancusa. Int. J. Biopharma Research, (2) : 97-100. ISSN: 2287-6898.
• Singh BR, Singh V, Singh RK, Ebibeni N. (2011). Antimicrobial activity of lemongrass (Cymbopogon citratus) oil against microbes of environmental, clinical and food origin. International Research of Pharmacy and Pharmacology,1(9) :228-236.
• Soares, M O et al. (2013). Cymbopogon citratus EO antimicrobial activity against multi-drug resistant Gram-positive strains and non-albicans-Candida species. Microbial pathogens and strategies for combating them: science, technology and education (A. Méndez-Vilas, Ed.) FORMATEX.