Kist Hidatitli Koyunlarda TAS ve TOS Düzeylerinin İncelenmesi

Yıl 2019, Cilt: 2 Sayı: 4 - Ek sayı, 164 - 168, 30.12.2019

Handan Mert Semih Yaşar , Nihat Mert

Öz

Etkeni Echinococcus granulosus’un larval formu olan kist hidatik hayvanlarda ve insanlarda yaygın olarak görülen paraziter bir zoonozdur. Hastalık, Türkiye’de halen endemik olarak seyretmekte olup oldukça önemli ekonomik kayıplara sebep olmaktadır. Bu çalışmada, kist hidatitli koyunlarda TAS ve TOS düzeyleri tespit edilerek, bu biyokimyasal parametrelerin kistik ekinokokkozisteki değişimleri araştırıldı. Araştırmanın materyalini, Van ili Özalp ilçesinde mezbahaneye getirilen ve kesimi yapılan 2-3 yaşlı Morkaraman koyunlar oluşturdu. Kesim öncesi koyunların genel sağlık durumları fiziki muayene ile kontrol edilerek kan örnekleri alındı. Kesim sonrası hayvanların değişik organlarında kist hidatik muayenesi yapıldı. Protoskoleks yönünden pozitif (fertil kist) olan 25 adet koyun çalışmanın deneme grubunu (kistik grup), organ muayenelerinde herhangi bir patolojik lezyon bulunmayan ve fiziki muayenede sağlıklı görünen 15 adet koyun ise kontrol grubunu oluşturdu. Sağlıklı ve kist hidatik ile enfekte hayvanlardan alınan kan örneklerinin serumları ayrıldı. Serum örneklerinde TAS ve TOS düzeyleri Spektroskopik metodlar ile ticari kitler kullanılarak belirlendi. Kontrol grubu koyunlarda TAS 1.67 ± 0.04 mmol trolox Equiv./L, kistik ekinokokozis ile enfekte koyunlarda 1.44 ± 0.05 mmol trolox Equiv./L (p<0.01)olarak saptanırken TOS düzeyleri kontrol grubunda 4.46 ± 0.58 μmol H2O2 equiv./L, kistik ekinokokozisli grupta 6.94 ± 0.59 μmol H2O2 equiv./L olarak bulundu (p<0.001). OSI değeri ise kontrol grubunda 0.27 ± 0.08 kistik ekinokokozis ile enfekte koyunlarda 0.48 ± 0,05 Arbitrary Unit olarak hesaplandı. Fagositik hücrelerin kistik bölgeye göçü sonucu aktivitelerine bağlı olarak daha fazla oksijen kullanılmasıyla lipit peroksidasyonu oluşmaktadır. Bunun sonucunda hidatik kistli koyunlarda TAS düzeylerinde azalma, TOS düzeylerinde ise artma gözlendi.

Anahtar Kelimeler

Ekinokok, Koyun, TAS, TOS

Investigation of TAS and TOS Levels in Sheep with Hydatid Cyst

Öz

Echinococcus granulosus is a larval form of the hydatid cyst is a parasitic zoonosis commonly seen in animals and humans. The disease is endemic in Turkey is still far leads to substantial economic losses. In this study, TAS and TOS levels will be determined in cyst hydatite sheep and the changes of these biochemical parameters in cystic echinococcosis were investigated. The material of the study was composed of 2-3 aged Morkaraman sheep which were slaughtered and slaughter house in the Özalp district of Van province. The general health status of the sheep before slaughtering was checked by physical examination and blood samples were taken. After slaughtered, cyst hydatid examination was performed in different organs of the animals. The experimental group (cystic group) of the 25 sheep study with a positive (fertile cyst) of protoscolex, and 15 sheep with no pathological lesion on organ examinations and healthy physical examination were the control group. Serum samples were taken from healthy and blood samples taken from animals infected with hydatid cyst. TAS and TOS levels in serum samples were determined by spectroscopic methods using commercial kits. In control group sheep had TAS 1.67 ± 0.04 mmol trolox Equiv./L and in sheep infected with cystic echinococcosis was 1.44 ± 0.05 mmol trolox Equiv./L (p<0.01), TOS levels were 4.46± 0.58 μmol H2O2 equiv./L in the control group and 6.94 ±0.59 μmol H2O2 equiv./L in the cystic echinococcosis group (p <0.001). OSI values were 0.27 ± 0.08 Arbitrary Unit in the control group and 0.48 ± 0.05 Arbitrary Unit in sheep infected with cystic echinococcosis. As a result of the migration of phagocytic cells to the cystic region, lipid peroxidation occurs due to the use of more oxygen. As a result, TAS levels decreased and TOS levels increased in sheep with hydatid cysts.

Anahtar Kelimeler

: Echinococcus, TAS, TOS, Sheep

Kaynakça

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