Isolation of Rhodotorula glutinis from Mammillaria elongata Fruit Extract and Its Determination with 18 S rRNA Gene Region

Yıl 2022, Cilt: 5 Sayı: 2, 53 - 58, 16.05.2022

Fatma Şeyma Gökdemir , Fatma Gönül Solmaz , Sumer Aras

https://doi.org/10.53493/avrasyasbd.1006290

Öz

Mammillaria elongata is a species of Cactaceae family, native to central Mexico. In Turkey, it is grown as cultivated plant. The succulent plants have pink and red fruits. In this study, we investigated the protein content, sugar content, anti-bacterial and anti-fungal effect in fruit extract. According to the results, in the fruit with a total wet weight of 0.252 g 15.5215 mg protein was found with Lowry method. Besides, the extract contains high amounts of reducing sugar such as glucose. Bacterial growth (Escherichia coli and Pseudomonas aeruginosa on bloody agar) and fungal colonization (Fusarium oxysporum, Rhizoctonia solani, Sclerotinia sclerotiorum on PDA) were not affected by plant extract. However, in all control media, that contain only plant extract, pink colored organism growth was observed. After DNA ısolation and 18S rRNA PCR from this organism, its species name determined as Rhodotorula glutinis according to % 100 sequence similarity.

Anahtar Kelimeler

mammilaria elongata, rhodotorula glutinis, 18S rRNA

Destekleyen Kurum

No ınstitution

Teşekkür

We would like to thanks Prof. Dr. Hamdi Güray Kutbay from Ondokuz Mayıs University.

Isolation of Rhodotorula glutinis from Mammillaria elongata Fruit Extract and Its Determination with 18S rRNA Gene Region

Öz

Mammilaria elongata Cactaceae failyasına ait ve ana vatanı Meksika olan bir kaktüs türüdür. Türkiye de ise kültür bitkisi olarak yetiştirilmektedir. Bu sukkulent bitkinin pembe ve kırmızı meyveleri bulunmaktadır. Bu çalışmada; bu bitkinin meyvelerinden elde edilen özütlerin şeker ve protein içerikleri, anti-bakteriyel ve anti-fungal etkileri araştırılmıştır. Sonuçlara göre; toplam yaş ağırlığı 0,252 g olan meyvelerde Lowry metodu ile 15,5215 mg protein bulunmaktadır. Bitki özütü yüksek miktarda glikoz gibi indirgen şeker içermektedir. Bitki özütü, kanlı agarda, Esherichia coli ve Pseudomonas aeruginosa üzerinde antibakteriyel; patates desktroz agarda (PDA) Fusarium oxysporum, Rhizoctonia solani, Sclerotinia sclerotiorum üzerinde antifungal bir etki göstermemiştir. Ancak, tüm kontrol besiyerlerinde pembe renkli bir organizmanın geliştiği gözlemlenmiştir. DNA izolasyonu ve 18S rRNA PCR’ı takiben bu organizmanın Rhodotorula glutinis ile % 100 sekans benzerliği gösterdiği belirlenmiştir.

Anahtar Kelimeler

Rhodotorula glutinis, 18S rRNA, Mammillaria elongata

Kaynakça

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