In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi

Göksel Özer; Muharrem Türkkan; Ferit Sönmez; Hüseyin Kabakcı; Mehtap Alkan; Sibel Derviş

doi:10.29278/azd.1452105

TR EN

Selenyumun Bazı Bitki Patojeni Funguslara Karşı In Vitro Değerlendirmesi

Abstract

Amaç: Selenyum (Se), tarımsal alanlarda fungal patojenlerini kontrol etmek için umut vaat eden çevre dostu bir element olarak ilgi çekmektedir. Bu çalışmada, sodyum selenit (selenit) ve sodyum selenat (selenat) formlarını içeren Se uygulamalarının 10 farklı bitki patojeni fungus türünün büyümesi üzerindeki etkisi değerlendirilmiştir. Materyal ve Yöntem: Selenyum uygulamalarının fungusların miselyal gelişmesi ve spor oluşumu üzerindeki etkisi in vitro koşullarda değerlendirilmiştir. Probit analizi, fungusların hem miselyal gelişme hem de spor oluşumunda %50 azalmaya (EC50) yol açan tuz konsantrasyonlarını belirlemek için kullanılmıştır. Araştırma Bulguları: En yüksek konsantrasyonda (120 ppm), selenit tüm türlerin miselyal gelişmesini %6.82 ile %62.46 arasında engellemiştir. Buna karşın, selenat daha geniş bir engelleme spektrumu göstermiş ve miselyum büyümesini %0 ile %87.14 arasında etkilemiştir. Farklı konsantrasyonlarda, Fusarium pseudograminearum selenit karşısında (EC50<24 ppm) en yüksek duyarlılığı gösterirken onu Bipolaris sorokiniana ve Verticillium dahliae izlemiştir. Benzer şekilde, Colletotrichum coccodes selenat uygulamasına karşı (EC50<24 ppm) en yüksek duyarlılığı gösterirken, onu B. sorokiniana, Botrytis cinerea, Sclerotinia sclerotiorum ve V. dahliae takip etmiştir. Her iki tuz da anlamlı bir farklılık gözlenmeksizin fungal türleri üzerinde spor oluşumunu etkili bir şekilde inhibe etmiştir. Colletotrichum coccodes, F. pseudograminearum, B. cinerea, F. culmorum, V. dahliae ve B. sorokiniana selenit tarafından anlamlı şekilde inhibe edilmiştir fakat F. oxysporum’a karşı daha düşük bir inhibisyon gözlenmiştir. Benzer şekilde, engelleme yüzdeleri arasında küçük farklar bulunmakla birlikte bu türler, V. dahliae ve F. oxysporum ile birlikte, selenat tarafından anlamlı şekilde inhibe edilmiştir. Colletotrichum coccodes, B. cinerea, F. culmorum, B. sorokiniana ve F. oxysporum için 24 ppm'nin altındaki EC50 değerleri, her iki tuzun da spor oluşumunu etkin bir şekilde inhibe ettiğini göstermiştir. Fusarium pseudograminearum’un %50 inhibisyonu için daha yüksek konsantrasyonların gerektiği anlaşılmıştır. Verticillium dahliae, selenit karşısında 33.16 ppm ve selenat karşısında 24 ppm'nin altında olan EC50 değerleri ile selenata karşı daha yüksek duyarlılık göstermiştir. Sonuç: Bu çalışmanın bulguları, Se'nin sürdürülebilir tarımda çeşitli bitki patojen fungus türleri üzerindeki antifungal potansiyeline ilişkin anlayışımıza katkıda bulunmaktadır. Hastalık yönetimi için elementin mekanizmalarını anlamak ve uygulama protokollerini optimize etmek için daha fazla araştırmaya ihtiyaç vardır.

Keywords

Na2SeO3, Na2SeO4, antifungal, miselyal gelişme, sporulasyon, EC50

In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi

Abstract

Objective: Selenium (Se) is garnering interest as a promising environmentally friendly element for controlling fungal pathogens in agricultural production. This study evaluated the impact of Se treatments, comprising sodium selenite (selenite) and sodium selenate (selenate) forms, on the growth of 10 plant pathogenic fungi. Materials and Methods: The impact of Se treatments on the mycelial growth and sporulation of fungi was assessed in in vitro conditions. Probit analysis was used to determine the concentrations of salts that induced a 50% reduction (EC50) in both mycelial growth and sporulation of fungi. Results: At the highest concentration (120 ppm), selenite demonstrated inhibitory effects on mycelial growth across various species, with a reduction in growth ranging from 6.82% to 62.46%. In contrast, selenate exhibited a broader spectrum of inhibition, affecting mycelial growth from 0% to 87.14%. Across different concentrations, Fusarium pseudograminearum displayed the highest sensitivity to selenite (EC50<24 ppm), followed by Bipolaris sorokiniana and Verticillium dahliae. Similarly, Colletotrichum coccodes exhibited the highest sensitivity to selenate treatment (EC50<24 ppm), followed by B. sorokiniana, Botrytis cinerea, Sclerotinia sclerotiorum, and V. dahliae. Both salts effectively inhibited sporulation across fungal species, with no significant difference observed. Colletotrichum coccodes, F. pseudograminearum, B. cinerea, F. culmorum, V. dahliae, and B. sorokiniana were significantly inhibited by selenite, while F. oxysporum exhibited lower inhibition. Similarly, these species, along with V. dahliae and F. oxysporum, were significantly inhibited by selenate, with slight differences between their inhibition percentages. EC50 values below 24 ppm were observed for C. coccodes, B. cinerea, F. culmorum, B. sorokiniana, and F. oxysporum, indicating potent inhibition of sporulation by both salts. Fusarium pseudograminearum required slightly higher concentrations for 50% inhibition. Verticillium dahliae showed higher sensitivity to selenate than selenite, with EC50 values of 33.16 ppm and below 24 ppm, respectively. Conclusion: The findings of this study contribute to our understanding of Se's antifungal potential across diverse plant pathogenic fungal species in sustainable agriculture. Further research is warranted to elucidate its mechanisms and optimize treatment protocols for disease management.

Keywords

Na2SeO3, Na2SeO4, antifungal, mycelial growth, sporulation, EC50

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Göksel Özer
0000-0002-3385-2520
Türkiye

Muharrem Türkkan
0000-0001-7779-9365
Türkiye

Ferit Sönmez
0000-0003-1437-4081
Türkiye

Hüseyin Kabakcı
0000-0002-9671-9858
Türkiye

Mehtap Alkan
0000-0002-7923-8892
Türkiye

Sibel Derviş ^*
0000-0002-4917-3813
Türkiye

Publication Date

July 5, 2024

Submission Date

March 15, 2024

Acceptance Date

May 24, 2024

Published in Issue

Year 2024 Volume: 13 Number: 1

DOI

https://doi.org/10.29278/azd.1452105

IZ

https://izlik.org/JA54ZT67SS

APA

Özer, G., Türkkan, M., Sönmez, F., Kabakcı, H., Alkan, M., & Derviş, S. (2024). In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi. Akademik Ziraat Dergisi, 13(1), 99-110. https://doi.org/10.29278/azd.1452105

AMA

1.Özer G, Türkkan M, Sönmez F, Kabakcı H, Alkan M, Derviş S. In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi. Akademik Ziraat Dergisi. 2024;13(1):99-110. doi:10.29278/azd.1452105

Chicago

Özer, Göksel, Muharrem Türkkan, Ferit Sönmez, Hüseyin Kabakcı, Mehtap Alkan, and Sibel Derviş. 2024. “In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi”. Akademik Ziraat Dergisi 13 (1): 99-110. https://doi.org/10.29278/azd.1452105.

EndNote

Özer G, Türkkan M, Sönmez F, Kabakcı H, Alkan M, Derviş S (July 1, 2024) In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi. Akademik Ziraat Dergisi 13 1 99–110.

IEEE

[1]G. Özer, M. Türkkan, F. Sönmez, H. Kabakcı, M. Alkan, and S. Derviş, “In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi”, Akademik Ziraat Dergisi, vol. 13, no. 1, pp. 99–110, July 2024, doi: 10.29278/azd.1452105.

ISNAD

Özer, Göksel - Türkkan, Muharrem - Sönmez, Ferit - Kabakcı, Hüseyin - Alkan, Mehtap - Derviş, Sibel. “In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi”. Akademik Ziraat Dergisi 13/1 (July 1, 2024): 99-110. https://doi.org/10.29278/azd.1452105.

JAMA

1.Özer G, Türkkan M, Sönmez F, Kabakcı H, Alkan M, Derviş S. In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi. Akademik Ziraat Dergisi. 2024;13:99–110.

MLA

Özer, Göksel, et al. “In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi”. Akademik Ziraat Dergisi, vol. 13, no. 1, July 2024, pp. 99-110, doi:10.29278/azd.1452105.

Vancouver

1.Göksel Özer, Muharrem Türkkan, Ferit Sönmez, Hüseyin Kabakcı, Mehtap Alkan, Sibel Derviş. In Vitro Evaluation of Selenium Against Some Plant Pathogenic Fungi. Akademik Ziraat Dergisi. 2024 Jul. 1;13(1):99-110. doi:10.29278/azd.1452105

Cited By

Inhibitory Effects and Underlying Mechanisms of a Selenium Compound Agent Against the Pathogenic Fungus Sclerotinia sclerotiorum Causing Sclerotinia Stem Rot in Brassica napus

Agronomy

https://doi.org/10.3390/agronomy15081764