Çeşitli Yerel Nane (Mentha piperita L.) Genotiplerinde Agronomik Özelliklerin ve Uçucu Yağ Veriminin Değerlendirilmesi

Year 2024, , 1 - 12, 05.07.2024

Andaç Kutay Saka , Adnan Uğur , Muhammed Akif Açıkgöz

https://doi.org/10.29278/azd.1465733

Abstract

Amaç: Mentha piperita L. içerdiği biyoaktif bileşikler nedeniyle tıp, ilaç endüstrisi, gıda ve kozmetik endüstrilerinde uzun süredir kullanılmaktadır. Bu çalışma Ordu ilinin farklı bölgelerinden toplanan nane genotiplerinde bazı agronomik özellikler ile uçucu yağ veriminin belirlenmesi amacıyla yürütülmüştür.
Materyal ve Yöntem: Çalışmada Ordu ilinin 16 farklı ilçesinden toplanan, toplam 44 farklı genotip kullanılmıştır. Köklenmiş nane çelikleri ısıtmasız plastik serada balkon tipi plastik saksılarda yetiştirilmiştir. Naneler 3 farklı dönemde hasat edilmiş ve bitkilerde sürgün boyu, yaprak eni, yaprak uzunluğu, renk özellikleri, yaprak klorofil indeksi, toplam verim ve uçucu yağ verimi belirlenmiştir. Bu özelliklere göre temel bileşen analizi yapılmıştır. Öklid benzerlik mesafesi kullanılarak, ağırlıksız çift grup yöntemi algoritması (UPGMA) kümeleme analizi gerçekleştirilmiş ve genotiplerin dendogramları oluşturulmuştur.
Araştırma Bulguları: Araştırmanın sonunda nane genotiplerinde bitki sürgün boyları 12.2-60.6 cm, yaprak eni 0.8-4.3 cm, yaprak uzunluğu 1.2-9.8 cm, yaprak klorofil indeksi 4.75-47.8 cci (SPAD), uçucu yağ verimi %0.45-2.9 ve toplam verim 0.1-4.2 t da-1 aralığında tespit edilmiştir. Yapılan temel bileşen analizi sonucunda ilk 4 temel bileşen ekseninin öz değeri 1’den büyük bulunmuştur. İlk 3 eksen diğer eksenlere göre yüksek varyasyon yüzdesine sahiptir. Oluşan dendogram sonucunda 2 grup, 4 alt grup meydana gelmiştir. Birbirine en yakın genotipler G12 ile G33, en uzak genotipler ise G10 ile G11 genotipleri olmuştur.
Sonuç: Araştırma sonucunda farklı nane genotiplerinin agronomik özellikleri ile uçucu yağ oranları belirlenerek ileride yapılacak ıslah çalışmalarına materyal olabilecek nitelikli genotipler belirlenmiştir.

Keywords

Kroma, Hue açısı, genotip, morfolojik özellikler, uçucu yağ

Ethical Statement

Çalışmada, Etik Kurul Onayına ihtiyaç bulunmamaktadır.

Assessment of Agronomic Traits and Essential Oil Yield in Various Local Mint (Mentha piperita L.) Genotypes

Abstract

Objective: Mentha piperita L. has long been used in medicine, pharmaceutical industry, food and cosmetic industries due to the bioactive compounds it contains. This study was carried out to determine some agronomic characteristics and essential oil yield of mint genotypes collected from different regions of Ordu province.
Materials and Methods: A total of 44 different genotypes collected from 16 different districts of Ordu province were used in the study. Rooted mint cuttings were grown in unheated plastic greenhouse in balcony type plastic pots. Mint plants were harvested in 3 different periods and shoot length, leaf width, leaf length, colour characteristics, leaf chlorophyll index, total yield and essential oil yield were determined. Principal component analyses were performed using the data obtained from these traits. Unweighted pair group method algorithm (UPGMA) clustering analysis was performed using Euclidean similarity distance and dendrograms of genotypes were formed.
Results: At the end of the study, plant length was 12.2-60.6 cm, leaf width 0.8-4.3 cm, leaf length 1.2-9.8 cm, leaf chlorophyll index 4.75-47.8 cci (SPAD), essential oil yield 0.45-2.9% and total yield 0.1-4.2 t da-1 in mint genotypes. As a result of the principal component analysis, the eigenvalues of the first 4 principal component axes were found to be greater than 1. The first 3 axes have high variation percentage compared to other axes. Mint genotypes were divided into 2 main groups and 4 subgroups in the UPGMA dendrogram. While G12 and G33 were the closest mint genotypes, G10 and G11 were found to be genetically distant genotypes.
Conclusion: In the study, genotypes that exhibit potential in terms of total yield and essential oil yield have been identified. As a result of the research, agronomic characteristics and essential oil ratios of different mint genotypes were determined and qualified genotypes that can be material for future breeding studies were determined.

Keywords

Chroma, Hue angle, landraces, morphological characteristics, volatile oil

References

• Açıkgöz, M. A. (2019). Evaluation of phytochemical compositions and biological properties of Achillea gypsicola at different phenological stages. Chemistry & Biodiversity, 16(12), e1900373.
• Açıkgöz, M. A. (2020). Determination of essential oil compositions and antimicrobial activity of Achillea gypsicola Hub.-Mor. at different plant parts and phenological stages. Journal of Essential Oil Research, 32(4), 331-347.
• Alvand, Z. M., Parseghian, L., Aliahmadi, A., Rahimi, M., & Rafati, H. (2024). Nanoencapsulated Thymus daenensis and Mentha piperita essential oil for bacterial and biofilm eradication using microfluidic technology. International Journal of Pharmaceutics, 651, 123751.
• Benabdallah, A., Boumendjel, M., Aissi, O., Rahmoune, C., Boussaid, M., & Messaoud, C. (2018). Chemical composition, antioxidant activity and acetylcholinesterase inhibitory of wild Mentha species from northeastern Algeria. South African Journal of Botany, 116, 131-139.
• Court, W. A., Pocs, R., Roy, R. C., 1993. Effect of harvest date on the yield and quality of the essential oil of peppermint. Canadian Journal of Plant Science, 73(3), 815-824.
• Derwich, E., Benziane, Z., Taouil, R., Senhaji, O., & Touzani, M. (2010). Aromatic plants of morocco: GC/MS analysis of the essential oils of leaves of Mentha piperita. Advances in Environmental Biology, 80-86.
• Devi, A., Iqbal, T., Wani, I. A., Sharma, G., Verma, S., Noureldeen, A., & Darwish, H. (2022). Assessment of variability among morphological and molecular characters in wild populations of mint (Mentha longifolia L.) germplasm. Saudi Journal of Biological Sciences, 29(5), 3528-3538.
• Duarte, H., Aliaño-González, M. J., Cantos-Villar, E., Faleiro, L., Romano, A., & Medronho, B. (2024). Sustainable extraction of polyphenols from vine shoots using deep eutectic solvents: Influence of the solvent, Vitis sp., and extraction technique. Talanta, 267, 125135.
• Gai, F., Janiak, M. A., Sulewska, K., Peiretti, P. G., & Karamać, M. (2023). Phenolic compound profile and antioxidant capacity of flax (Linum usitatissimum L.) harvested at different growth stages. Molecules, 28(4), 1807.
• Gupta, A. K., Mishra, R., Singh, A. K., Srivastava, A., & Lal, R. K. (2017). Genetic variability and correlations of essential oil yield with agro-economic traits in Mentha species and identification of promising cultivars. Industrial Crops and Products, 95, 726-732.
• Hammer, Ø., Harper, D. A., & Ryan, P. D. (2001). Past: paleontological statistics software package for educaton and data anlysis. Palaeontologia Electronica, 4(1), 1-9.
• Hoque, A., Rumman, R., Akter, K. T., Choudhury, T. I., & Faisal, W. (2014). Morphological Characterization and Evaluation of Twenty Two Mint Germplasm. Research & Reviews: Journal of Crop Science and Technology, 4, 4-12.
• Hussain, A. I., Anwar, F., Nigam, P. S., Ashraf, M., & Gilani, A. H. (2010). Seasonal variation in content, chemical composition and antimicrobial and cytotoxic activities of essential oils from four Mentha species. Journal of the Science of Food and Agriculture, 90(11), 1827-1836.
• Iezzoni, A. F., & Pritts, M.P. (1991). Applications of principal component analysis to horticultural research. HortScience, 26(4), 334-338.
• Jahan, N., Hussain, N., Touqeer, S. I., Shamshad, H., & Abbas, N. (2024). Formulation of Mentha piperita-Based Nanobiopesticides and Assessment of the Pesticidal and Antimicrobial Potential. Life, 14(1), 144.
• Kabir, S., Tahira, R., Khan, M. F., Naeemullah, M., & Jamal, A. (2014). Molecular characterization of genetic diversity of mint gene pool through RAPD markers. International Journal of Agricultural Innovations and Research, 2(4), 2319-1473.
• Kecis, H., Abdelouahab, Y., Bagues, M., Gali, L., Mekircha, F., Alloun, W., & Nagaz, K. (2023). Phenolic profile and bioactivity of the aerial part and roots of Mentha rotundifolia L. grown in two different localities in northeastern Algeria: A comparative study. Biocatalysis and Agricultural Biotechnology, 47, 102581.
• Lianah, l., Kusumarini, N., Hafshah, M., Krisantini, K., Kurniawati, A., & Ahmad, M. U. (2023). Chemical characterization of mint (Mentha spp.) germplasm from Central Java, Indonesia. Biodiversitas Journal of Biological Diversity, 24(8), 4307-4313.
• Lu, L., Cao, H., Li, H., Zhang, H., Li, S., & Wang, J. (2022). Diversity and profiles of volatile compounds in twenty-five peppermint genotypes grown in China. International Journal of Food Properties, 25(1), 1472-1484.
• Mcguire, R. G., 1992. Reporting of objective color measurements. Hort-Science, 27,1254-1255.
• Mehdizadeh, L., Moghaddam, M., Ganjeali, A., & Rahimmalek, M. (2024). Phenolic compounds, enzymatic and non-enzymatic antioxidant activities of Mentha piperita modified by Zinc and methyl jasmonate concentrations. Scientia Horticulturae, 329, 112980.
• Moghaddam, M., Pourbaige, M., Tabar, H. K., Farhadi, N., & Hosseini, S. M. A. (2013). Composition and antifungal activity of peppermint (Mentha piperita) essential oil from Iran. Journal of Essential Oil Bearing Plants, 16(4), 506-512.
• Mohammadi, H., Parviz, L., Beyrami, A., Anosheh-Bonab, F., & Ghorbanpour, M. (2024). Exposure to TiO2 nanoparticles (NPs) and zeolite stimulates growth, physiology, and phytochemical characteristics and elevates Mentha piperita L. tolerance to salinity stress. Industrial Crops and Products, 211, 118228.
• Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89(3), 583-590.
• Ostadi, A., Javanmard, A., Machiani, M. A., Morshedloo, M. R., Nouraein, M., Rasouli, F., Maggi, F., (2020). Effect of different fertilizer sources and harvesting time on the growth characteristics, nutrient uptakes, essential oil productivity and composition of Mentha x piperita L. Industrial Crops and Products, 148, 112290.
• Panjeshahin, Z., Sharifi-Sirchi, G. R., & Samsampour, D. (2018). Genetic and Morphological Diversity of Wild Mint. Journal of Medicinal plants and By-product, 7(1), 105-115.
• Piccaglia, R., Dellacecca, V., Marotti, M., Giovanelli, E., (1993). Agronomic factors affecting the yields and the essential oil composition of peppermint (Mentha x Pıperıta l.). In International Symposium on Medicinal and Aromatic Plants (29-40).
• Pinto, L., Tapia-Rodríguez, M. R., Baruzzi, F., & Ayala-Zavala, J. F. (2023). Plant antimicrobials for food quality and safety: Recent views and future challenges. Foods, 12(12), 2315.
• Reynders, S., & Bollereau, P. (1994). Characterization of genetic diversity in Rosa by randomly amplified polymorphic DNA. Acta Horticulturae, 424, 253-260.
• Rohloff, J., Dragland, S., Mordal, R., & Iversen, T. H. (2005). Effect of harvest time and drying method on biomass production, essential oil yield and quality of peppermint (Mentha piperita L.). Journal of Agriculture Food Chemistry, 2005,53(10), 4143-4148.
• Roshanibakhsh, F., Samsampour, D., Askari Seyahooei, M., & Bagheri, A. (2023). Strong relationship between molecular and morphological attributes in Iranian mentha populations (Mentha mozaffarianii Jamzad). Genetic Resources and Crop Evolution, 70(6), 1721-1745.
• Singh, R., Shushni, M. A. M., & Belkheir, A., 2015. Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry, 8: 322–328.
• Singh, V. P., Singh, M., & Singh, D. V. (1998). Growth, yield and quality of peppermint (Mentha x piperita L.) as influenced by planting time. Journal of Herbs, Spices Medicinal Plants, 5(3), 33-39. DOI: 10.1300/J044v05n03_05
• Soilhi, Z., Trindade, H., Vicente, S., Gouiaa, S., Khoudi, H., & Mekki, M. (2020). Assessment of the genetic diversity and relationships of a collection of Mentha spp. in Tunisia using morphological traits and ISSR markers. The Journal of Horticultural Science and Biotechnology, 95(4), 483-495.
• Tabar, F. M., Fathi, S., Shameh, S., & Alirezaloo, A. (2024). Investigating Morphological Diversity, Essential Oil and Antioxidant Activity of some wild Mentha longifolia L. Genotypes in North-West of Iran. Journal of Crop Breeding, 16(49), 129-139.
• Telci, I. (2001). Farklı nane (Mentha spp) klonlarının bazı morfolojik, tarımsal ve teknolojik özelliklerinin belirlenmesi üzerinde bir araştırma. Gaziosmanpaşa Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, Tokat.
• Telci, I., & Sahbaz, N. (2005). Determination of agronomic and essential oil properties of peppermint (Mentha piperita L.) in various ages of plantation. Journal of Agronomy, 103-108.
• Telci, I., Bayram, E., Yılmaz, G., & Avcı, B. (2006). Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Biochemical Systematics and Ecology, 34, 489-497.
• Telci, I., Sahbaz, N. I., Yilmaz, G., & Tugay, M. E. (2004). Agronomical and chemical characterization of spearmint (Mentha spicata L.) originating in Turkey. Economic Botany, 58(4), 721-728.
• Verma, R. S., Rahman, L., Verma, R. K., Chauhan, A., Yadav, A. K., & Singh, A., (2010). Essential oil composition of menthol mint (Mentha arvensis) and peppermint (Mentha piperita) cultivars at different stages of plant growth from Kumaon region of Western Himalaya. Journal of Medicinal and Aromatic Plants, 1(1), 13-18.
• White, J. G. H., Iskandar, S. H., & Barnes, M. F. (1987). Peppermint: effect of time of harvest on yield and quality of oil. New Zealand Journal of Experimental Agriculture, 15(1), 73-79. DOI: 10.1080/03015521.1987.10425539
• Yeşil, M. (2021). Mentha piperita L. hidrosolünün Echinacea purpurea (L.) Moench’nın çimlenme performansına etkisi. Akademik Ziraat Dergisi, 10(2), 405-410. https://doi.org/10.29278/azd.949187
• Yeşil, M., & Kara, K. (2015). Mentha spicata L. ve Mentha villoso-nervata Opiz. genotiplerinin tarımsal özellikleri üzerine azot ve fosfor dozlarının etkisi. Akademik Ziraat Dergisi, 3(1), 23-32.
• Yeşil, M., & Kara, K. (2017). Mentha spicata L. ve Mentha villoso-nervata L. genotiplerinin bazı uçucu yağ bileşenleri üzerine farklı azot ve fosfor dozlarının etkisi. Akademik Ziraat Dergisi, 6(2), 131-140. https://doi.org/10.29278/azd.370787
• Yeşil, M., Öner, E. K., & Özcan, M. M. (2018). Ordu ekolojik şartlarında farklı nane (Mentha sp.) türlerinin tarımsal özelliklerinin belirlenmesi. Turkish Journal of Agriculture-Food Science and Technology, 6(12), 1734-1740.
• Zheljazkov, V. D., Cantrell, C. L., Astathie, T., & Hristov, A. (2010). Yield, Content, and Composition of Peppermint and Spearmints as a Function of Harvesting Time and Drying. Journal of Agricultural and Food Chemistry, 58(21), 11400-11407.