Multi regression analysis of the effect of potassium bicarbonate on in vitro the mycelial growth and sclerotial germination of Botrytis cinerea

Öz

In this study, the effects of time and doses of potassium bicarbonate (KHCO3) on mycelial growth and sclerotial germination of Botrytis cinerea was evaluated by mathematical modeling with multi regression analysis. All equations produced for mycelial growth and sclerotial germination of B. cinerea were derived as affected by doses and times. ANOVA and multi-regression analysis showed a close relationship between actual and predicted mycelial growth and sclerotial germination of B. cinerea. The models developed to predict mycelial growth and sclerotial germination were, respectively, MG=(a)–(b x T2) + [c x (D x T)] and SG=(a)+(b x T)–[c x (D x T)]+[d x (D² x T)], where a, b, c and d represent co-efficients obtained through multi regression analysis, T represents time and D represents dosage. R2 values of mycelial growth and sclerotial germination were 0.83 and 0.81, respectively. Standard errors were found to be significant at p<0.001.

Anahtar Kelimeler

• Botrytis cinerea,potassium bicarbonate,multi-regression analysis

References

1. Agrios, G. N., 2005. Plant Pathology, 5th. Academic Press, Inc.NewYork. 805 pp.
2. Bisiach, M., Minervini, G., Vercessi, A., 1984. Biological and epidemiological aspects of the kiwifruit (Actinida chinensis Planchon) rot, caused by Botrytis cinerea Pers. Rivista Di Patologia Vegetale Serie IV. 20: 38–55.
3. Brook, P. J., 1991. Botrytis stem end rot and other storage diseases of kiwifruit – a review. Acta Horticulturae 297: 545–550.
4. Bulit, J. and Dubos, B., 1988. Botrytis bunch rot and blight. In; Pear-Son, R.C. and Goheen, A.C. (eds)
5. Compendium of Grape Deiseases, The American Phytopathological Society, St.Paul, Minessota. pp. 13-15.
6. Çalışkan, O., Odabaş, M. S., Çırak, C., Radusiene, J. and Odabaş, F., 2010. The quantitative effect of the temperature and light intensity at growth in Origanum onites L. J Medicinal Plants Research 4(7): 551-558.
7. Cuppers, H. G. A. M., Ooomes, S. and Brul, S., 1997. A model for the combined effects of temperature and salt concentration on growth rate of food spoilage moulds. Applied and Environmental Microbiol 63: 3764–3769.
8. Elad, Y., Williamson, B., Tudzynski, P. and Delen, N., 2007. Botrytis spp. and diseases they cause in agricultural systems–an introduction. pp. 1–24. In ‘Botrytis: biology, pathology and control’. (EdsYElad, B Williamson, P Tudzynski, N Delen) Springer.

9. Erkan, M., Demir, T., Öz, S. and Delen, N., 1997. Investigations on the sensitivities of gray mold (Botrytis cinerea) isolates on grapes against some fungicides. J.Turk. Phytopath 26(2-3): 87-96.
10. Gabler, M. and Smilanick, J. L., 2001. Postharvest Control of Table Grape Gray Mold on Detached Berries with Carbonate and Bicarbonate Salts and Disinfectants. Am. J. Enol. Vitic. 52:12-20.
11. Ieki, H., 1993. Kiwifruit diseases in Japan. Japan Pesticide Information 61: 11–13.
12. Judet-Correia, D., Bollaert, S., Duquenne, A., Charpentier, C., Bensoussan, M. and Dantigny, P., 2010. Validation of a predictive model for the growth of Botrytis cinerea and Penicillium expansum on grape berries. International J Food Microbiol. 142: 106–113.
13. Karabulut, O. A., Romanazzi, G., Smilanick, J. L. and Lichter, A., 2005. Postharvest ethanol and potassium sorbate treatments of table grapes to control gray mold. Postharvest Biol & Technol 37(2): 129-134.
14. Karakaya, A. and Bayraktar, H., 2009. Botrytis disease of kiwifruit in Turkey. Australasian Plant Disease Notes 4: 87-88.
15. Lahlali, R., Serrhini, M. N., Friel, D. and Jijakli, M. H., 2007. Predictive modelling of temperature and water activity (solutes) on the in vitro radial growth of B. cinerea. International J Food Microbiol 114: 1–9.
16. Marín, S., Sanchis, V., Teixidó, A., Saenz, R., Ramos, A. J., Vinas, I. and Magan, N., 1996. Water and temperature relations and microconidial germination of Fusarium moniliforme and F. proliferatum from maize. Canadian J Food Microbiol 42, 1045–1050.
17. McMeekin, T. A., Olley, J. N., Ratkowsky, D. A. and Ross, T., 2002. Predictive microbiology: towards the interface and beyond. International J Food Microbiol 73, 395–407.
18. Michailides, T. J, and Emler, P. A. G., 2000. Botrytis gray mold of kiwifruit caused by Botrytis cinerea in the United States and New Zealand. Plant Disease 84: 208–223.
19. Türkkan, M. 2014. Evaluation of Inhibitory Effect of Organic and Inorganic Salts Against Ilyonectria liriodendri, The Causal Agent of Root Rot Disease of Kiwifruit. Journal of Phytopathology, doi: 10.1111/jph.12355.
20. Odabaş, M. S., Radusiene, J., Cirak, C. and Camas, C., 2009. Models of estimation of the content of secondary metabolites in some Hypericum species. Pharmaceutical Biol 47(2), 1117-1122.
21. Ordonez, C., Alarcón, A., Ferrera, R. and Hernández, L. V., 2009. In vitro antifungal effects of potassium bicarbonate on Trichoderma sp. and B. cinerea. Mycoscience 50: 380–387.
22. Palmer, C. L., Horst, R. K. and Langhans, R. W., 1997. Use of bicarbonates to inhibit in vitro colony growth of Botrytis cinerea. Plant Disease 81: 432-1438.
23. Ricker, M. D. and Punja, Z., 1991. Influence of Fungicide and Chemical Salt Dip Treatments on Crater Rot Caused by Rhizoctonia carotae in Long-Term Storage. Plant Disease 75: 470-474.
24. Sautour, M. S., Mansur, C., Divies, C., Bensoussan, M. and Dantigny, P., 2002. Comparison of the effects of temperature and water activity on growth rate of food spoilage moulds. J Industrial Microbiol and Biotechnol 28: 311–316.
25. Smilanick, J. L., Mansour, M. F. and Sorenson, D., 2006. Pre- and postharvest treatments to control green mould of citrus fruit during ethylene degreasing. Plant Dis. 90: 89-96.
26. Weisberg, S., 2005. Applied linear regression. New York, USA, 310 p.
27. Yıldırım, I. and Yapıcı, B. M., 2007. Inhibition of conidia germination and mycellium growth of Botrytis cinerea by some alternative chemicals. Pakistan J Biological Sciences 10(8): 1294-1300.
28. Zhang, J. and Swingle, P., 2003. Control of Green Mold on Florida Citrus Fruit Using Bicarbonate Salts. Proc. Fla. State Hort. Soc. 116: 375-378.