Isolation and characterization of Bacillus megaterium isolates from dead pentatomids and their insecticidal activity to Palomena prasina nymphs

Hasan Murat Aksoy; Celal Tuncer; İslam Saruhan; İsmail Erper; Murat Öztürk; İzzet Akca

doi:10.29278/azd.440586

Isolation and characterization of Bacillus megaterium isolates from dead pentatomids and their insecticidal activity to Palomena prasina nymphs

Öz

Bacillus megaterium isolates, were demonstrated to be efficient biocontrol agents against the green shield bug (Palomena prasina L., Heteroptera: Pentatomidae). Firstly hazelnut orchards were surveyed and four B. megaterium isolates were obtained from P. prasina. The morphological, physiological and biochemical characteristics of B. megaterium isolates were determined according to the standardized methodology. Additionally 16S rRNA gene sequence analyse was performed to determine the isolates. Analysis of the 16S rRNA gene confirmed that isolates Sa-1, Sa-5, SAk-2 and SAkc-19 are B. megaterium, with 100% sequence homology to the type strains of B. megaterium. Effectiveness of these isolates was tested against P. prasina nymphs in laboratory, at 25±1°C and 70±5 RH. Isolates were bioassayed against nymphs and dead individuals were counted daily, following a 12 days lasting treatment. Lethal time (LT₅₀ and LT₉₀) values of B. megaterium isolates were calculated. LT₅₀ values of the two most active isolates were 1.91 and 11.18 days. The highest mortality rate was 98% obtained with the treatment of SAkc-2 isolates at concentrations of 10⁸ cfu ml^-1 on the 12^th day of post treatment.

Anahtar Kelimeler

Bacillus megaterium,Palomena prasina,biological control,entomopathogen

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Hasan Murat Aksoy This is me

Celal Tuncer This is me

İslam Saruhan ^*

İsmail Erper This is me

Murat Öztürk This is me

İzzet Akca This is me

Publication Date

July 15, 2018

Submission Date

December 19, 2017

Acceptance Date

-

Published in Issue

Year 2018 Volume: 7 Number: 1

DOI

https://doi.org/10.29278/azd.440586

IZ

https://izlik.org/JA23ZG43ND

Cite

RIS / Bibtex

APA

Aksoy, H. M., Tuncer, C., Saruhan, İ., Erper, İ., Öztürk, M., & Akca, İ. (2018). Isolation and characterization of Bacillus megaterium isolates from dead pentatomids and their insecticidal activity to Palomena prasina nymphs. Akademik Ziraat Dergisi, 7(1), 21-28. https://doi.org/10.29278/azd.440586

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