Isolation and characterization of Bacillus megaterium isolates from dead pentatomids and their insecticidal activity to Palomena prasina nymphs

Yıl 2018, , 21 - 28, 15.07.2018

Hasan Murat Aksoy Celal Tuncer İslam Saruhan , İsmail Erper Murat Öztürk İzzet Akca

https://doi.org/10.29278/azd.440586

Cited By: 2

Öz

Bacillus megaterium isolates, were
demonstrated to be efficient biocontrol agents against the green shield bug (Palomena prasina L., Heteroptera:
Pentatomidae). Firstly hazelnut orchards were surveyed and four B. megaterium isolates were obtained
from P. prasina. The morphological,
physiological and biochemical characteristics of B. megaterium isolates were determined according to the
standardized methodology. Additionally 16S rRNA gene sequence analyse was
performed to determine the isolates. Analysis of the 16S rRNA gene confirmed
that isolates Sa-1, Sa-5, SAk-2 and SAkc-19 are B. megaterium, with 100% sequence homology to the type strains of B. megaterium. Effectiveness of these
isolates was tested against P. prasina
nymphs in laboratory, at 25±1°C and 70±5 RH. Isolates were bioassayed against
nymphs and dead individuals were counted daily, following a 12 days lasting
treatment. Lethal time (LT₅₀ and LT₉₀) values of B. megaterium isolates were calculated.
LT₅₀ values of the two most active isolates were 1.91 and 11.18
days. The highest mortality rate was 98% obtained with the treatment of SAkc-2
isolates at concentrations of 10⁸ cfu ml^-1 on the 12^th
day of post treatment.

Anahtar Kelimeler

Bacillus megaterium, Palomena prasina, biological control, entomopathogen

Kaynakça

• Abbott, W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18 : 265-267.
• Aksoy, H. M., Ozman-Sullivan, S. K., 2008. Isolation of Bacillus megaterium from Aphis pomi (Homoptera: Aphididae) and Assessment of Its Pathogenicity. Journal of Plant Pathology, 90: 433-436.
• Al-Rehiayani, S., Hafez, S. L., Thornton, M., 1999. Effects of Pratylenchus neglectus, Bacillus megaterium, and oil radish or rapeseed green manure on reproductive potential of Meloidogyne chitwoodi on potato. Nematropica, 29: 37–49.
• Anonymous, 2016a. Fındık raporu, Şubat 2015, Ankara. T.C. Gümrük ve Ticaret Bakanlığı, Kooperatifçilik Genel Müdürlüğü, 29p.
• Anonymous, 2016b. 2014 Yılı Fındık sektör raporu. Toprak Mahsulleri Ofisi Genel Müdürlüğü.
• Buresova, V., Franta, Z., Kopacek P. 2006. A comparison of Chryseobacterium indologenes pathogenicity to the soft tick Ornithodoros moubata and hard tick Lxodes ricinus. Journal of Invertebrate Pathology, 93: 96-104.
• Cavados, C. F. G., Fonseca, R. N., Chaves, Q., Rabinovitch, L., Araujo-Coutinho, C. J. P. C., 2001. Identification of entomopathogenic Bacillus isolated from Simulium (Diptera, Simuliidae) larvae. Memorias Instituto Oswaldo Cruz, 96: 1017-1021.
• Gokce, C., Sevim, A., Demirbag, Z., Demir I. 2010. Isolation, characterization and pathogenicity of bacteria from Rhynchites bacchus (Coleoptera: Rhynchitidae). Biocontrol Science and Technology, 20: 973-982.
• Gu, Y. Q., Zhou, J. P., Mo, M. H., Zhang, K. Q., 2007. Evaluation and identification of potential organic nematocidal nematocidal volatiles from soil bacteria. Soil Biology and Biochemistry, 39: 2567-2575.
• Huang, Y., Ma, L., Xu, C., Zhang, K., Duan, C., Mo, M. 2010. Characterisation of volatiles produced from Bacillus megaterium YFM3.25 and their nematicidal activity against Meloidogyne incognita. European Journal of Plant Pathology, 126: 417-422.
• Khyami-Horani, H., Katbeh-Bader, A., Mohsen, Z. H. 1999. Isolation of endospore-forming bacilli toxic to Culiseta longiareolata (Diptera: Culicidae) in Jordan. Letters in Applied Microbiology, 28: 57-60.
• Logan, N. A., Vos, D. P. 2009. Genus I. Bacillus Cohn 1872. In: The Firmicutes, Bergey's Manual of Systematic Bacteriology 2nd ed 3:21–128, Springer, New York, NY, USA.
• Neipp, P. W., Becker, J. O., 1999. Evaluation of biocontrol activity of rhizobacteria from Beta vulgaris against Heterodera schachtii. Journal of Nematology, 31: 54-61.
• Oliveira, D.F., Campos, V.P., Amaral, D.R., Nunes, A.S., Pantaleo, J.A., Costa, D.A. 2007. Selection of rhizobacteria able to produce metabolites active against Meloidogyne exigua. European Journal of Plant Pathology, 119: 477-479.
• Ozsahin, E., Sezen, K., Demir, I., Demirbag Z., 2014. Bacterial isolates from Palomena prasina (Hemiptera: Pentatomidae) include potential microbial control agents. Biocontrol Science and Technology, 24: 1039-1051.
• Padgham, J. L., Sikora, R. A., 2007. Biological control potential and modes of action of Bacillus megaterium against Meloidogyne graminicola on rice. Crop Protection, 26: 971-977.
• Patricia, S. V., Biedendieck, R., Fuerch, T., Meinhardt F., Rohde, M., Deckwer, W. D., Jahn, D., 2007. Bacillus megaterium-from simple soil bacterium to industrial protein production host. Applied Microbiology and Biotechnology, 76: 957–967.
• Pietrantonio, P. V., Federici, B. A., Gill, S. S., 1993. Interaction of Bacillus thuringiensis endotoxin with the insect midgut epithelium. In: Beckage N., Thompson, S. N., Federici, B. A. (eds.). 1: 55-79. Parasites and Pathogens of Insects. Academic Press, New York, NY, USA.
• Robertson, J. L., Russell, R. M., Preisler H.K., Savin, E., 2007. Bioassays with Arthropods 2nd Edn. CRC Press, Boca Raton, FL (US).
• Sezen, K., Demir, I., Demirbag, Z., 2004. Study of the bacterial flora as a biological control agent of Agelastica alni L. (Coleoptera: Chrysomelidae). Biologia, 59: 327–331.
• Slepecky, R. A., Hemphill, H. E., 2006. The Genus Bacillus-Nonmedical. Prokaryotes, 4: 530-562.
• Sneath, P. H. A. 1986. Endospore-forming gram positive Rods and Cocci. 1104-1105 In: Sneath, P. H. A., Mour N. S., Sharpe M. E., Holt J. G. (eds). Bergy's Manual of Systematic Bacteriology 8th Edition William & Wilkins Baltimore.
• Tamura, K., Stecher, G., Peterson, D., Kumar, S. 2013. MEGA 6:Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology Evolution, 30:2725-2729.
• Tavella, L., Arzone, A. M., Miaja, L., Sonnati, C. 2001. Influence of Bug (Heteroptera, Coreidae and Pentatomidae) feeding activity on hazelnut in Northwest Italy. Acta Horticulturae, 556: 461-468.
• Throne, J. E., Weaver, D. K., Chew, V., Baker, J. E., 1995. Probit analysis of correlated data: Multiple observations over time at one concentration. Journal of Economic Entomology, 88: 1510-1512.
• Tuncer, C., Akça, I., Saruhan, I. 2002. Findikta zararlı olan bazı emici boceklerin (Heteroptera: Pentatomidae, Coreidae ve Acanthosomatidae) kimyasal mucadelesi uzerine arastirmalar. Ondokuz Mayis Universitesi Ziraat Fakultesi Dergisi, 17: 17-26.
• Tuncer, C., Saruhan, I., Akca, I. 2004. The Insect Pest Problem Affecting Hazelnut Kernel Quality in Turkey. 6. International Hazelnut Congress. Tarragona, Spain, 14-18 June 2004.
• Tuncer, C., Saruhan, I., Akca, I. 2005. The Insect Pest Problem Affecting Hazelnut Kernel Quality in Turkey. Acta Horticulturae, 668: 367-376.
• Tuncer, C., Saruhan I., Akca I. 2009. Chemical Control of True Bugs (Heteroptera: Pentatomidae, Acanthosomatidae and Coreidae) For Preventing Kernel Damage in Hazelnut Orchards of Turkey. Acta Horticulturae, 845: 487-494.
• Wagner, D. L., Peacock, J. M., Carter J.L., Talley, S. E. 1996. Field assessment of Bacillus thuringiensis on non-target Lepidoptera. Environmental Entomology, 25: 1444-1454.
• Weisberg, W. G., Barns, S. M., Pelletier, D. A., Lane, D. J. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697–703.
• Zhang, H., Deng, W., Yu, Z. 1995. A review on the progresses of controlling stored product insects with Bacillus thuringiensis. Chinese Journal of Biological Control, 11: 178-182.