Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Year 2018, , 179 - 184, 26.12.2018

Muhammed Akif Açıkgöz Şevket Metin Kara , Ebru Batı Ay Sedanur Odabaş

https://doi.org/10.29278/azd.476349

Cited By: 2

Abstract

Light is not only
effective in photosynthesis, growth and development, but also play an important
role in biosynthesis of primary and secondary metabolites. The present research
was aimed to determine effect of light on biosynthesis of alkamide, caffeic
acid derivatives and echinacoside in cell suspension cultures of Echinacea purpurea L. Stem explants
derived from sterile plants were subjected to callus culture in a B5 media
supplemented with 1.0 mg/L BAP and 2.0 mg/L NAA. Afterward, 8-day cell cultures
were incubated in light and dark environments and cell harvesting, with a
three-day interval, was carried out five times. The contents of alkamide,
caffeic acid derivatives and echinacoside were determined using HPLC. Alkamide,
caftaric acid and echinacoside content increased regularly with light
application. Light application increased alkamide content by 57%, as an
average, compared to darkness. The cell cultures exposed to 12 days of
incubation in light produced the highest alkamide content, with a 2.11-fold
increase, compared to dark. Subjecting the cell cultures to light for 12 days
resulted in 70% and 63% increases in caftaric acid and echinacoside. This study
showed that light application in Echinacea
purpurea L. cell suspension culture had a great potential for increasing some
important phytochemicals.

Keywords

Caftaric acid, elicitor, purple coneflower, phytochemical compounds, secondary metabolites

References

• Ahmad, N., Rab, A., Ahmad, N. 2016. Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert). Journal of Photochemistry and Photobiology B: Biology, 154: 51-56.
• Ali, M., Abbasi, B.H. 2014. Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B: Biology, 140: 223-227.
• Balcerowicz, M., Fittinghoff, K., Wirthmueller, L., Maier, A., Fackendahl, P., Fiene, G., Koncz, C., Hoecker, U. 2011. Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2. Plant Journal, 65: 712–723.
• Bennett, R.N., Wallsgrove R.M. 1994. Secondary metabolites in plant defense mechanisms. New Phytologist, 127:617-33.
• Chan, L.K., Koay, S.S., Boey, P.L., Bhatt, A. 2010. Effects of abiotic stress on biomass and anthocyanin production in cell cultures of Melastoma malabathricum. Biological Research, 43:127-135.
• Chaves, I., Pokorny, R., Byrdin, M., Hoang, N., Ritz, T., Brettel, K., Ahmad, M. 2011. The cryptochromes: blue light photoreceptors in plants and animals. Annual Review of Plant Biology, 62: 335-364.
• El-Aal, M.S.A., Rabie, K.A.E., Manaf Hossam, H. 2016. The Effect of UV-C on Secondary Metabolites Productıon of Echinacea purpurea Culture in Vitro. Environmental Science & Technology, 11(2): 465-483.
• Gehlot, A., Arya, I.D., Arya, S. 2017. Regeneration of Complete Plantlets from Callus Culture of Azadirachta indica A. Juss using Immature Flower Buds. Advances in Forestry Science, 4(1): 71-76.
• Georgieva, L., Ivanov, I., Marchev, A., Aneva, I., Denev, P., Georgiev, V., Pavlov, A. 2015. Protopine production by Fumaria cell suspension cultures: effect of light. Applied Biochemistry and Biotechnology, 176(1): 287-300.
• Gualandi, R.J., Augé, R.M., Kopsell, D.A., Ownley, B.H., Chen, F., Toler, H.D., Gwinn, K.D. 2014. Fungal mutualists enhance growth and phytochemical content in Echinacea purpurea. Symbiosis, 63(3): 111-121.
• Halliday, K.J., Fankhauser, C. 2003. Phytochrome‐hormonal signaling networks. New Phytologist, 157(3): 449-463.
• Heijde, M., Ulm, R. 2012. UV-B photoreceptor-mediated signaling in plants. Trends in Plant Science, 17(4): 230-237.
• Kami, C., Lorrain, S., Hornitschek, P., Fankhauser, C. 2010. Chapter two-light-regulated plant growth and development. Current Topics in Developmental Biology, 91: 29-66.
• Khan, M.A., Abbasi, B.H., Ahmed, N., Ali, H. 2013. Effects of light regimes on in vitro seed germination and silymarin content in Silybum marianum. Industrial Crops and Products, 46: 105-110.
• Laloue, M., Courtois, D., Manigault, P. 1980. Convenient and rapid fluorescent staining of plant cell nuclei with “33258” Hoechst. Plant Science Letter, 17: 175-179.
• Liu, R., Li, W., Sun, L.Y., Liu, C.Z. 2012. Improving root growth and cichoric acid derivatives production in hairy root culture of Echinacea purpurea by ultrasound treatment. Biochemical Engineering Journal, 60: 62-66.
• Manayi A., Mahdi, V., Soodabeh, S. 2016. Echinacea purpurea: Pharmacology, phytochemistry and analysis methods. Pharmacognosy Reviews, 9(17): 63.
• Moroff, G., Eich, J., Dabay, M. 1994. Validation of use of the nageotte: hemocytometer to count low levels of white cells in white cell reduced platelet components. Transfusion, 34: 35-38.
• Murthy, H. N., Dandin, V.S., Zhong, J.J., Paek, K.Y. 2014. Strategies for enhanced production of plant secondary metabolites from cell and organ cultures. In Production of Biomass and Bioactive Compounds Using Bioreactor Technology, eds. K.Y. Paek, H.N. Murthy and J.J. Zhong, pp. 471-508. Springer, Dordrecht, the Netherlands.
• Romero, F.R., Delate, K., Kraus, G.A., Solco, A.K., Murphy, P.A., Hannapel, D.J. 2009. Alkamide production from hairy root cultures of Echinacea. In Vitro Cellular & Developmental Biology Plant, 45(5): 599-604.
• Shalitin, D., Yang, H., Mockler, T.C., Maymon, M., Guo, H., Whitelam, G.C., Lin, C. 2002. Regulation of Arabidopsis cryptochrome 2 by blue-light-dependent phosphorylation. Nature, 417(6890): 763-767.
• Shohael, A.M., Ali, M.B., Yu, K.W., Hahn, E.J., Islam, R., Paek, K.Y. 2006. Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochemistry, 41(5): 1179-1185.
• Sreelakshmi, Y., Sharma, R. 2008. Differential regulation of phenylalanine ammonia lyase activity and protein level by light in tomato seedlings. Plant Physiology and Biochemistry, 46(4): 444-451.
• Srivastava, P., Sisodia, V., Chaturvedi, R. 2011. Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L. Bioprocess and Biosystems Engineering, 34(1): 75-80.
• Tariq, U., Ali, M., Abbasi, B.H. 2014. Morphogenic and biochemical variations under different spectral lights in callus cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B: Biology, 130: 264-271.
• Weidler, G., Zur Oven-Krockhaus, S., Heunemann, M., Orth, C., Schleifenbaum, F., Harter, K., Hoecker, U., Batschauer, A. 2012. Degradation of Arabidopsis CRY2 is regulated by SPA proteins and phytochrome A. Plant Cell, 24: 2610–2623.
• Winkel-Shirley, B. 2001. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiology, 126(2): 485-493.
• Wu, C.H., Murthy, H.N., Hahn, E.J., Paek, K.Y. 2007. Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlorogenic acid and caftaric acid. Biotechnology Letters, 29(8): 1179-1182.
• Xu, C.G., Tang, T.X., Chen, R., Liang, C.H., Liu, X.Y., Wu, C.L., Wu, H.A. 2014. A comparative study of bioactive secondary metabolite production in diploid and tetraploid Echinacea purpurea (L.) Moench. Plant Cell, Tissue and Organ Culture (PCTOC), 116(3): 323-332.
• Zhao, S.Z., Sun, H.Z., Chen, M., Wang, B.S. 2010. Light-regulated betacyanin accumulation in euhalophyte Suaeda salsa calli. Plant Cell, Tissue and Organ Culture (PCTOC), 102(1): 99-107.