Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Muhammed Akif Açıkgöz; Şevket Metin Kara; Ebru Batı Ay; Sedanur Odabaş

doi:10.29278/azd.476349

Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Öz

Light is not only effective in photosynthesis, growth and development, but also play an important role in biosynthesis of primary and secondary metabolites. The present research was aimed to determine effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in cell suspension cultures of Echinacea purpurea L. Stem explants derived from sterile plants were subjected to callus culture in a B5 media supplemented with 1.0 mg/L BAP and 2.0 mg/L NAA. Afterward, 8-day cell cultures were incubated in light and dark environments and cell harvesting, with a three-day interval, was carried out five times. The contents of alkamide, caffeic acid derivatives and echinacoside were determined using HPLC. Alkamide, caftaric acid and echinacoside content increased regularly with light application. Light application increased alkamide content by 57%, as an average, compared to darkness. The cell cultures exposed to 12 days of incubation in light produced the highest alkamide content, with a 2.11-fold increase, compared to dark. Subjecting the cell cultures to light for 12 days resulted in 70% and 63% increases in caftaric acid and echinacoside. This study showed that light application in Echinacea purpurea L. cell suspension culture had a great potential for increasing some important phytochemicals.

Anahtar Kelimeler

Caftaric acid,elicitor,purple coneflower,phytochemical compounds,secondary metabolites

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Muhammed Akif Açıkgöz Bu kişi benim

Şevket Metin Kara ^*

Ebru Batı Ay Bu kişi benim

Sedanur Odabaş Bu kişi benim

Yayımlanma Tarihi

26 Aralık 2018

Gönderilme Tarihi

4 Haziran 2018

Kabul Tarihi

18 Ekim 2018

Yayımlandığı Sayı

Yıl 2018 Cilt: 7 Sayı: 2

DOI

https://doi.org/10.29278/azd.476349

IZ

https://izlik.org/JA24XN98XN

Kaynak Göster

RIS / Bibtex

APA

Açıkgöz, M. A., Kara, Ş. M., Batı Ay, E., & Odabaş, S. (2018). Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures. Akademik Ziraat Dergisi, 7(2), 179-184. https://doi.org/10.29278/azd.476349

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