Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook

Ebru Batı Ay; Muhammed Akif Açıkgöz; Beril Kocaman; Şevket Metin Kara

doi:10.29278/azd.781534

TR EN

Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook

Abstract

Galanthus elwesii Hook is a medically and economically important species that contains acetylcholinesterase inhibitory alkaloids galantamine and lycorine with well-known biological activities. In a field experiment, different plant parts of G. elwesii were harvested at three growing stages: flowering, post-flowering and fruit set. A comparative evaluation of maceration and ultrasonic-assisted extraction of galantamine and lycorine from these plant parts was implemented. The antioxidant activities and the contents of total phenolic and flavonoid of different plant parts were also investigated. The quantfication of galantamine and lycorine in the extracts was carried out by Reversed-phase High-Performance Liquid Chromatography. The HPLC analysis showed that ultrasonic-assisted extraction displayed higher quantities of galantamine, lycorine, total phenolic compounds and flavonoid and stronger antioxidant activity than maceration extraction. The highest amount of galantamine and lycorine was found in the root and leaf samples taken at fruit set as 89.27 and 23.20 µg g-1, respectively. Further, the leaf and bulb samples of fruit formation yielded the highest total phenolic and flavonoid contents of 45.58 and 60.92 GAE g-1 DW, respectively. In addition, higher antioxidant activities were observed with the leaf and bulb samples harvested at the stage of fruit set.

Keywords

Acetylcholinesterase,, Amaryllidaceae, Antioxidant activity, Snowdrop

Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook

Abstract

Galanthus elwesii Hook is a medically and economically important species that contains acetylcholinesterase inhibitory alkaloids galantamine and lycorine with well-known biological activities. In a field experiment, different plant parts of G. elwesii were harvested at three growing stages: flowering, post-flowering and fruit set. A comparative evaluation of maceration and ultrasonic-assisted extraction of galantamine and lycorine from these plant parts was implemented. The antioxidant activities and the contents of total phenolic and flavonoid of different plant parts were also investigated. The quantfication of galantamine and lycorine in the extracts was carried out by Reversed-phase High-Performance Liquid Chromatography. The HPLC analysis showed that ultrasonic-assisted extraction displayed higher quantities of galantamine, lycorine, total phenolic compounds and flavonoid and stronger antioxidant activity than maceration extraction. The highest amount of galantamine and lycorine was found in the root and leaf samples taken at fruit set as 89.27 and 23.20 µg g-1, respectively. Further, the leaf and bulb samples of fruit formation yielded the highest total phenolic and flavonoid contents of 45.58 and 60.92 GAE g-1 DW, respectively. In addition, higher antioxidant activities were observed with the leaf and bulb samples harvested at the stage of fruit set.

Keywords

Acetylcholinesterase, Amaryllidaceae, Antioxidant activity, Snowdrop

References

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Ebru Batı Ay ^*
0000-0002-9210-6907
Türkiye

Muhammed Akif Açıkgöz
Türkiye

Beril Kocaman
Türkiye

Şevket Metin Kara
Türkiye

Publication Date

December 30, 2020

Submission Date

August 17, 2020

Acceptance Date

November 6, 2020

Published in Issue

Year 2020 Volume: 9 Number: 2

DOI

https://doi.org/10.29278/azd.781534

IZ

https://izlik.org/JA92EN96EC

APA

Batı Ay, E., Açıkgöz, M. A., Kocaman, B., & Kara, Ş. M. (2020). Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook. Akademik Ziraat Dergisi, 9(2), 297-306. https://doi.org/10.29278/azd.781534

AMA

1.Batı Ay E, Açıkgöz MA, Kocaman B, Kara ŞM. Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook. Akademik Ziraat Dergisi. 2020;9(2):297-306. doi:10.29278/azd.781534

Chicago

Batı Ay, Ebru, Muhammed Akif Açıkgöz, Beril Kocaman, and Şevket Metin Kara. 2020. “Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook”. Akademik Ziraat Dergisi 9 (2): 297-306. https://doi.org/10.29278/azd.781534.

EndNote

Batı Ay E, Açıkgöz MA, Kocaman B, Kara ŞM (December 1, 2020) Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook. Akademik Ziraat Dergisi 9 2 297–306.

IEEE

[1]E. Batı Ay, M. A. Açıkgöz, B. Kocaman, and Ş. M. Kara, “Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook”, Akademik Ziraat Dergisi, vol. 9, no. 2, pp. 297–306, Dec. 2020, doi: 10.29278/azd.781534.

ISNAD

Batı Ay, Ebru - Açıkgöz, Muhammed Akif - Kocaman, Beril - Kara, Şevket Metin. “Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook”. Akademik Ziraat Dergisi 9/2 (December 1, 2020): 297-306. https://doi.org/10.29278/azd.781534.

JAMA

1.Batı Ay E, Açıkgöz MA, Kocaman B, Kara ŞM. Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook. Akademik Ziraat Dergisi. 2020;9:297–306.

MLA

Batı Ay, Ebru, et al. “Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook”. Akademik Ziraat Dergisi, vol. 9, no. 2, Dec. 2020, pp. 297-06, doi:10.29278/azd.781534.

Vancouver

1.Ebru Batı Ay, Muhammed Akif Açıkgöz, Beril Kocaman, Şevket Metin Kara. Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook. Akademik Ziraat Dergisi. 2020 Dec. 1;9(2):297-306. doi:10.29278/azd.781534

Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook

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Comperative Study of Maceration and Ultrasonic-Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of Galanthus Elwesii Hook

Abstract

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