Preparation and Characterization of Sodium Alginate/Quaternized Carboxymethyl Cellulose Biofilms

Abstract

The increasing use of polymeric materials brings along some waste storage and environmental problems. Therefore, the interest in biomaterials is increasing day by day. In this study, it was aimed to develop and characterize sodium alginate/quaternized carboxymethyl cellulose biofilms. For this purpose, firstly, quaternized carboxymethyl cellulose (CMC) was synthesized and biofilms containing different ratios of sodium alginate and quaternized CMC were prepared. The antibacterial properties of the prepared biofilm materials were determined by the zone of inhibition (disk diffusion) method. The light transmittance of the materials was determined in the wavelength range of 190-1100 nm using ultraviolet (UV) and visible region spectrophotometer. In addition, the gel content of the biofilms was determined by the soxhlet extraction method. It is seen that the antimicrobial properties of the developed biofilms are improved by using quaternized CMC. It is clearly seen that the light transmittance decreases with the increase of quaternized CMC ratio in formulations at all wavelengths. High gel contents indicate that sodium alginate and quaternized CMC used in biofilm formulations are stable against organic solvents in harmony. When all the results obtained are evaluated, it is understood that the produced biofilms can be used as coatings on materials that need antimicrobial surfaces.

Keywords

• Quaternized carboxymethyl cellulose
• sodium alginate
• biofilms
• characterization

Sodyum Aljinat/Katyonik Karboksimetil Selüloz Biyofilmlerin Hazırlanması ve Karakterizasyonu

Öz

Polimerik malzeme kullanımının giderek artması bir takım atık depolama ve çevresel sorunları da beraberinde getirmektedir. Bu nedenle biyomalzemelere olan ilgi gün geçtikçe artmaktadır. Bu çalışmada, sodyum aljinat/kuarternize karboksimetil selüloz biyofilmlerin geliştirilmesi ve karakterizasyonu amaçlanmıştır. Bu amaç için ilk olarak kuarternize karboksimetil selüloz (CMC) sentezlenmiş ve farklı oranlarda soydum aljinat ve kuarternize CMC içeren biyofilmler hazırlanmıştır. Hazırlanan biyofilm malzemelerinin antibakteriyel özellikleri inhibisyon zonu (disk difüzyon) yöntemi ile belirlendi. Ultraviyole (UV) ve görünür bölge spektrofotometresi kullanılarak 190-1100 nm dalga boyu aralığında malzemelerin ışık geçirgenlikleri belirlendi. Ayrıca biyofilmlerin jel içeriği soxhlet ekstraksiyonu yöntemi ile belirlendi. Geliştirilen biyofilmlerin antimikrobiyal özelliklerinin kuarternize CMC kullanılmasıyla geliştirildiği görülmektedir. Tüm dalga boylarında formülasyonlar içerisinde kuarternize CMC oranın artması ile ışık geçirgenliğinin azaldığı açık bir şekilde görülmektedir. Yüksek jel içerikleri biyofilm formülasyonlarında kullanılan sodyum aljinat ve kuarternize CMC’nin uyum içerisinde, organik çözücülere karşı kararlı bir yapıda olduklarını göstermektedir. Elde edilen tüm sonuçlar değerlendirildiğinde, üretilen biyofilmlerin antimikrobiyal yüzeylere gereksinim duyulan malzemelerde kaplama olarak kullanılabileceğini anlaşılmaktadır.

Anahtar Kelimeler

• Katyonik karboksimetil selüloz
• sodyum aljinat
• biyo filmler
• karakterizasyon

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