Phytochemical analyses of Ebenus haussknechtii flowers: Quantification of phenolics, antioxidants effect, and molecular docking studies

Year 2024, , 1 - 9, 31.08.2024

Ramazan Erenler , İlyas Yıldız , Esma Nur Geçer , Aslı Yıldırım Kocaman , Mehmet Hakkı Alma , İbrahim Demirtas , Yunus Başar , İbrahim Hosaflıoğlu , Lütfi Behçet

https://doi.org/10.53445/batd.1479874

Abstract

Plants have been benefited as medicine and food since ancient times. After the discovery of spectroscopy, bioactive compounds in plants have been elucidated and have been utilized in drug development. Ebenus haussknechtii has been utilized for traditional medicine. In this study, Ebenus haussknechtii flowers were extracted in methanol and quantification of phenolics of this extract was conducted by LC-MS/MS. Antioxidant effect of E. haussknechtii flowers was carried out using DPPH free radical scavenging assay, ABTS radical cation scavenging assay, and hydroxyl radical scavenging assay. Quantitative analysis revealed that shikimic acid (0.77 mg/g extract), protocatechuic acid (0.61), catechin (0.34), hydroxybenzaldeyde (0.32) were determined as major products. Hence, the interaction of shikimic acid and DNA gyrase enzyme was calculated theoretically. Moreover, MolDock score, and binding affinity were determined as -73.64 and -5.5 kcal/mol respectively. Ebenus haussknechtii flowers displayed good antioxidant activity. In DPPH assay, the extract displayed good activity with the value of 7.27 ± 0.173 (IC50, µg/mL). Moreover, the flower extract exhibited the outstanding ABTS activity with a value of 6.62 ± 0.23 (IC50, µg/mL) in comparison to the extract BHA (7.58 ± 0.15, IC50, µg/mL).

Keywords

Ebenus haussknechtii, LC-MS/MS, Molecular docking, Antioxidant activity

Ebenus haussknechtii çiçeklerinin fitokimyasal analizleri: Fenoliklerin miktarının belirlenmesi, antioksidan etkisi ve moleküler yerleştirme çalışmaları

Abstract

Bitkilerden eski çağlardan beri ilaç ve gıda olarak yararlanılmaktadır. Spektroskopinin keşfinden sonra bitkilerde bulunan biyoaktif bileşikler aydınlatılmış ve ilaç geliştirmede kullanılmaya başlanmıştır. Ebenus haussknechtii geleneksel tıpta kullanılmaktadır. Bu çalışmada, Ebenus haussknechtii çiçekleri metanol içerisinde ekstrakte edilmiş ve bu ekstraktın fenoliklerinin miktar tayini LC-MS/MS ile yapılmıştır. E. haussknechtii çiçeklerinin antioksidan etkisi, DPPH serbest radikal giderme testi, ABTS radikal katyon giderme testi ve hidroksil radikal giderme testi kullanılarak gerçekleştirildi. Kantitatif analizde şikimik asit (0,77 mg/g ekstrakt), protokatekuik asit (0,61), kateşin (0,34), hidroksibenzaldeidin (0,32) ana ürünler olduğu belirlendi. Böylece şikimik asit ile DNA giraz enziminin etkileşimi teorik olarak hesaplandı. Ayrıca MolDock skoru ve bağlanma afinitesi sırasıyla -73,64 ve -5,5 kcal/mol olarak belirlendi. E. haussknechtii çiçekleri iyi antioksidan aktivite sergiledi. DPPH testinde ekstrakt 7.27 ± 0.173 (IC50, µg/mL) değeriyle iyi aktivite sergiledi. Ayrıca çiçek ekstraktı, BHA (7.58 ± 0.15, IC50, µg/mL) ile karşılaştırıldığında 6.62 ± 0.23 (IC50, µg/mL) değeriyle olağanüstü ABTS aktivitesi sergiledi.

Keywords

Ebenus haussknechtii, LC-MS/MS, Moleküler doking, Antioksidan aktivite

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