Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds

Ramazan Erenler; İlyas Yıldız; Esma Nur Geçer; İbrahim Hosaflıoğlu

doi:10.53445/batd.1769742

EN TR

Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds

Abstract

Since ancient times, people have used plants as food and medicine. Bioactive chemicals in plants have been identified and used in medicinal development since the discovery of spectroscopy. Nanotechnology is a branch of science that has developed rapidly in recent years due to its widespread application. In this study, silver nanoparticles (AgNPs@Mo) were synthesized by a green approach using the Melilotus officinalis leaves. The structure of the nanoparticle was identified by analytical techniques. The maximum absorption at 483 nm confirmed the formation of silver nanoparticles. The TEM image presented the morphology and particle size of nanoparticles (36.3 nm). The XRD spectrum determined the shape of nanoparticles as a face-centered cubic structure (fcc). The particle size was also determined by the Scherrer equation as 32.4 nm. The zeta potential (-31.1 mV) revealed the high stability of nanoparticles. Quantitative analysis of phenolic compounds in M. officinalis leaves was determined by LC-MS/MS, and coumarin (2.167 µg/g extract), syringic acid (0.027), salicylic acid (0.025), and rutin (0.066) were defined as major compounds. Melilotus officinalis leaves may be a valuable source for silver nanoparticle synthesis.

Keywords

Melilotus officinalis, nanoparticles, spectroscopy, bioactive compounds

Melilotus officinalis yaprakları üzerinde fitokimyasal çalışma: Gümüş nanopartiküllerin yeşil sentezi ve fenolik bileşiklerin kantitatif analizi

Abstract

Antik çağlardan beri insanlar bitkileri gıda ve ilaç olarak kullanmıştır. Spektroskopinin keşfinden bu yana bitkilerdeki biyoaktif kimyasallar tanımlanmakta ve ilaç geliştirmede kullanılmaktadır. Nanoteknoloji, yaygın kullanım alanları nedeniyle son yıllarda hızla gelişen bir bilim dalıdır. Bu çalışmada, Melilotus officinalis yaprakları kullanılarak yeşil yaklaşımla gümüş nanopartiküller (AgNPs@Mo) sentezlenmiştir. Nanopartikülün yapısı analitik tekniklerle tanımlanmıştır. 483 nm'de maksimum absorbsiyon, gümüş nanopartiküllerin oluşumunu doğrulamıştır. TEM görüntüsü, nanopartiküllerin morfolojisini ve parçacık boyutunu (36,3 nm) belirlemiştir. XRD spektrumu, nanopartiküllerin şeklini yüzey merkezli kübik yapı (fcc) olarak belirlemiştir. Parçacık boyutu da Scherrer denklemi ile 32,4 nm olarak belirlenmiştir. Zeta potansiyeli (-31,1 mV) nanopartiküllerin yüksek kararlılığını ortaya koymuştur. M. officinalis yapraklarındaki fenolik bileşiklerin kantitatif analizi LC-MS/MS ile yapıldı ve kumarin (2,167 µg/g ekstrakt), siringik asit (0,027), salisilik asit (0,025) ve rutin (0.066) başlıca bileşikler olarak tanımlandı. Melilotus officinalis yaprakları, gümüş nanopartikül sentezi için değerli bir kaynak olabilir.

Keywords

Melilotus officinalis, nanopartikül, spektroskopi, biyoaktif bileşikler

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Details

Primary Language

English

Subjects

Biomaterial

Journal Section

Research Article

Authors

Ramazan Erenler ^*
0000-0002-0505-3190
Türkiye

İlyas Yıldız
0000-0003-1254-1069
Türkiye

Esma Nur Geçer
0000-0002-0095-079X
Türkiye

İbrahim Hosaflıoğlu
0009-0000-2730-1493
Türkiye

Publication Date

August 30, 2025

Submission Date

August 21, 2025

Acceptance Date

August 22, 2025

Published in Issue

Year 2025 Volume: 6 Number: 2

DOI

https://doi.org/10.53445/batd.1769742

IZ

https://izlik.org/JA64LT95BH

APA

Erenler, R., Yıldız, İ., Geçer, E. N., & Hosaflıoğlu, İ. (2025). Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds. Journal of Integrative and Anatolian Medicine, 6(2), 114-120. https://doi.org/10.53445/batd.1769742

AMA

1.Erenler R, Yıldız İ, Geçer EN, Hosaflıoğlu İ. Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds. J. Integr. Anatol. Med. 2025;6(2):114-120. doi:10.53445/batd.1769742

Chicago

Erenler, Ramazan, İlyas Yıldız, Esma Nur Geçer, and İbrahim Hosaflıoğlu. 2025. “Phytochemical Study on Melilotus Officinalis Leaves: Green Synthesis of Silver Nanoparticles and Quantitative Analysis of Phenolic Compounds”. Journal of Integrative and Anatolian Medicine 6 (2): 114-20. https://doi.org/10.53445/batd.1769742.

EndNote

Erenler R, Yıldız İ, Geçer EN, Hosaflıoğlu İ (August 1, 2025) Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds. Journal of Integrative and Anatolian Medicine 6 2 114–120.

IEEE

[1]R. Erenler, İ. Yıldız, E. N. Geçer, and İ. Hosaflıoğlu, “Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds”, J. Integr. Anatol. Med., vol. 6, no. 2, pp. 114–120, Aug. 2025, doi: 10.53445/batd.1769742.

ISNAD

Erenler, Ramazan - Yıldız, İlyas - Geçer, Esma Nur - Hosaflıoğlu, İbrahim. “Phytochemical Study on Melilotus Officinalis Leaves: Green Synthesis of Silver Nanoparticles and Quantitative Analysis of Phenolic Compounds”. Journal of Integrative and Anatolian Medicine 6/2 (August 1, 2025): 114-120. https://doi.org/10.53445/batd.1769742.

JAMA

1.Erenler R, Yıldız İ, Geçer EN, Hosaflıoğlu İ. Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds. J. Integr. Anatol. Med. 2025;6:114–120.

MLA

Erenler, Ramazan, et al. “Phytochemical Study on Melilotus Officinalis Leaves: Green Synthesis of Silver Nanoparticles and Quantitative Analysis of Phenolic Compounds”. Journal of Integrative and Anatolian Medicine, vol. 6, no. 2, Aug. 2025, pp. 114-20, doi:10.53445/batd.1769742.

Vancouver

1.Ramazan Erenler, İlyas Yıldız, Esma Nur Geçer, İbrahim Hosaflıoğlu. Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds. J. Integr. Anatol. Med. 2025 Aug. 1;6(2):114-20. doi:10.53445/batd.1769742

J. Integr. Anatol. Med. (Abbreviated Title)

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e-ISSN: 2687-6345

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Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds

Abstract

Keywords

Melilotus officinalis yaprakları üzerinde fitokimyasal çalışma: Gümüş nanopartiküllerin yeşil sentezi ve fenolik bileşiklerin kantitatif analizi

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

J. Integr. Anatol. Med. (Abbreviated Title)