Phytochemical study on Melilotus officinalis leaves: Green synthesis of silver nanoparticles and quantitative analysis of phenolic compounds

Year 2025, Volume: 6 Issue: 2, 114 - 120, 30.08.2025

Ramazan Erenler , İlyas Yıldız , Esma Nur Geçer , İbrahim Hosaflıoğlu

https://doi.org/10.53445/batd.1769742

Abstract

Since ancient times, people have used plants as food and medicine. Bioactive chemicals in plants have been identified and used in medicinal development since the discovery of spectroscopy. Nanotechnology is a branch of science that has developed rapidly in recent years due to its widespread application. In this study, silver nanoparticles (AgNPs@Mo) were synthesized by a green approach using the Melilotus officinalis leaves. The structure of the nanoparticle was identified by analytical techniques. The maximum absorption at 483 nm confirmed the formation of silver nanoparticles. The TEM image presented the morphology and particle size of nanoparticles (36.3 nm). The XRD spectrum determined the shape of nanoparticles as a face-centered cubic structure (fcc). The particle size was also determined by the Scherrer equation as 32.4 nm. The zeta potential (-31.1 mV) revealed the high stability of nanoparticles. Quantitative analysis of phenolic compounds in M. officinalis leaves was determined by LC-MS/MS, and coumarin (2.167 µg/g extract), syringic acid (0.027), salicylic acid (0.025), and rutin (0.066) were defined as major compounds. Melilotus officinalis leaves may be a valuable source for silver nanoparticle synthesis.

Keywords

Melilotus officinalis , nanoparticles , spectroscopy , bioactive compounds

Melilotus officinalis yaprakları üzerinde fitokimyasal çalışma: Gümüş nanopartiküllerin yeşil sentezi ve fenolik bileşiklerin kantitatif analizi

Abstract

Antik çağlardan beri insanlar bitkileri gıda ve ilaç olarak kullanmıştır. Spektroskopinin keşfinden bu yana bitkilerdeki biyoaktif kimyasallar tanımlanmakta ve ilaç geliştirmede kullanılmaktadır. Nanoteknoloji, yaygın kullanım alanları nedeniyle son yıllarda hızla gelişen bir bilim dalıdır. Bu çalışmada, Melilotus officinalis yaprakları kullanılarak yeşil yaklaşımla gümüş nanopartiküller (AgNPs@Mo) sentezlenmiştir. Nanopartikülün yapısı analitik tekniklerle tanımlanmıştır. 483 nm'de maksimum absorbsiyon, gümüş nanopartiküllerin oluşumunu doğrulamıştır. TEM görüntüsü, nanopartiküllerin morfolojisini ve parçacık boyutunu (36,3 nm) belirlemiştir. XRD spektrumu, nanopartiküllerin şeklini yüzey merkezli kübik yapı (fcc) olarak belirlemiştir. Parçacık boyutu da Scherrer denklemi ile 32,4 nm olarak belirlenmiştir. Zeta potansiyeli (-31,1 mV) nanopartiküllerin yüksek kararlılığını ortaya koymuştur. M. officinalis yapraklarındaki fenolik bileşiklerin kantitatif analizi LC-MS/MS ile yapıldı ve kumarin (2,167 µg/g ekstrakt), siringik asit (0,027), salisilik asit (0,025) ve rutin (0.066) başlıca bileşikler olarak tanımlandı. Melilotus officinalis yaprakları, gümüş nanopartikül sentezi için değerli bir kaynak olabilir.

Keywords

Melilotus officinalis , nanopartikül , spektroskopi , biyoaktif bileşikler

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