Production of Nostoc commune loaded pH sensitive carrier microspheres and analysis of release kinetics at different pH values

Year 2022, Volume: 24 Issue: 2, 581 - 593, 08.07.2022

Cankız Gizem DELİBALTA Ezgi EREN BELGİN Cansu KORKMAZ Hilal GÖNEN Hüseyin ÇİÇEK

Abstract

Microspheres are carriers used for controlled drug release, carrying the active substance in the form of particles at the molecular level, and their diameters can vary from a few microns to millimeters. Microspheres can make controlled release depending on the pH change. In this study, pH sensitive drug carrier microspheres were produced using natural gelatin and sodium alginate polymers. N. commune extract, which has been reported to have anticancer effects in the literature and to be used as an antitumor agent in colon cancer, was prepared and loaded with approximately 0.12 mg per cell. In order to examine the microsphere fragmentation and drug release properties in the gastrointestinal system, spectrophotometric release kinetics studies were carried out in different pH environments. In conclusion, it was observed that the release of the extract is fastest at the pH of the colon and the microspheres have the potential to be used in colon cancer therapy for drug targeting.

Keywords

Nostoc commune, microspheres, pH sensitives, release kinetics, controlled release

Nostoc commune yüklü pH duyarlı taşıyıcı mikrokürelerin üretimi ve farklı pH değerlerindeki salım kinetiklerinin incelenmesi

Abstract

Mikroküreler, kontrollü ilaç salımı için kullanılan, etkin maddeyi moleküler düzeyde partiküller şeklinde taşıyan ve çapları birkaç mikrondan milimetreye kadar değişebilen taşıyıcılardır. Mikroküreler, pH değişimine bağlı olarak kontrollü salım yapabilmektedir. Bu çalışmada, doğal jelatin ve sodyum aljinat polimerleri kullanılarak pH duyarlı ilaç taşıyıcı mikroküreler üretilmiştir. Mikrokürelere literatürde antikanser etkileri olduğu ve kolon kanserinde antitümör ajanı olarak kullanımının gelecek vadettiği raporlanan N. commune ekstraktı hazırlanarak hücre başına yaklaşık 0,12 mg yüklenmiştir. Gastrointestinal sistemde mikroküre parçalanma ve etkin madde salım özelliklerinin incelenmesi için farklı pH ortamlarında spektrofotometrik olarak salım kinetiği çalışmaları yapılmıştır. Sonuçta, ekstrakt salınımının kolonun pH'ında en hızlı olduğu ve mikrokürelerin etken madde hedefleme için kolon kanseri tedavisinde kullanılma potansiyeline sahip olduğu görülmüştür.

Keywords

Nostoc commune, mikroküre, pH duyarlı küre, salım kinetiği, kontrollü salım

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