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Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi

Year 2023, Volume: 25 Issue: 1, 203 - 214, 16.01.2023
https://doi.org/10.25092/baunfbed.1056444

Abstract

Bu çalışmada Scardinius erythrophthalmus (Kızılkanat) sindirim kanalı mukozasında bulunan bazı peptitlerin yerleşim ve yoğunluklarının belirlenmesi amaçlanmıştır. İmmunohistokimysal incelemeler doğrultusunda CCK immunoreaktif hücrelerin midede bulunmadığı ancak ilk, orta ve son bağırsakta bulunduğu gözlendi. Mide ve orta bağırsakta gastrin immunopozitif hücreler gözlenmezken, ilk bağırsakta bu hücreler tespit edildi. Son bağırsakta gastrin immunoreaktif hücrelere az sayıda rastlandı. Glukagon immunoreaktif hücrelere midede rastlanmazken, bağırsakta az miktarda saptandı. Midede somatostatin immunopozitif hücrelerin az yoğunlukta olduğu, bağırsakta ise bulunmadığı gözlendi. Sonuç olarak peptitlerin bağırsaktaki yoğunluğunun değiştiği belirlendi.

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References

  • Vieira-Lopes, D.A., Pinheiro, N. L., Sales, A., Ventura, A., Araújo, F. G., Gomes, I. D. ve Nascimento, A. A., Immunohistochemical study of the digestive tract of Oligosarcus hepsetus, World Journal of Gastroenterology, 19 (12), 1919-1929, (2013).
  • Lee, C. S. ve Kaestner, K. H., Development of gut endocrine cells, Journal of Clinical Endocrinology and Metabolism, 18, 453–462, (2004).
  • Şenol, N. ve Çınar, K., Immunohistochemical localization of cholecystokinin and histamine in gastrointestinal tract in flower fısh (Pseudophoxinus antalyae), Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi, 1(1-2), 26-34, (2006).
  • Şenol, N., Sazan (Cyprinus carpio) ve Sudak (Stizostedion lucioperca) balıklarında gastrointestinal kanalın histokimyasal yapısı ve bazı peptidlerin lokalizasyonu, Doktora tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Isparta, (2009).
  • Blanco, A. M., Bertucci, J. I., Valenciano, A. I., Delgado, M. J. ve Unniappan, S., Ghrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) in the intestine, and attenuates the anorectic effects of CCK, PYY and GLP-1 in goldfish (Carassius auratus), Hormones and Behaviour, 93, 62–71, (2017).
  • Cardosoa, N. N., Firmianoa, E. M. S., Gomes, I. D., Nascimentoa, A., Sales, A. ve Araúj, F. G., Histochemical and immunohistochemical study on endocrine cells (5HT, GAS, and SST) of the gastrointestinal tract of a teleost, the characin Astyanax bimaculatus, Acta Histochemica, 117, 595–604, (2015).
  • Lee, J. H., Ku, S. K. Park, K. D. ve Lee, H. S., Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch, Journal of Fish Biology, 65, 170–181, (2004).
  • Losada, A. P., Bermudez, R. ve Failde, L. D., Giancamillo, A. D., Domeneghini C. ve Quiroga, M. I., Effects of Enteromyxum scophthalmi experimental infection on the neuroendocrine system of turbot, Scophthalmus maximus (L.), Fish and Shellfish Immunology, 40 (2), 577–583, (2014).
  • Özen, M. R., Çınar, K. ve Şenol, N., Endocrine cells in the gastrointestinal tract mucosa of zander (Stizostedion lucioperca), Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16 (Suppl-B): S339-S345, (2010).
  • Chena, M., He, M., Peng, K., Liu, T., Jin, C., Cao, W., Wang L. ve Xiao, K. An immunohistochemical study of somatostatin in the stomach and the small intestine of the African ostrich (Struthio camelus), Tissue and Cell, 45, 363–366, (2013).
  • Pereira, R. T., Costa, L.S., Oliveira, I. R., Araujo, J. C., Aerts, M., Vigliano, F. A. ve Rosa, P. V., Relative distribution of gastrin-, CCK-8-, NPY- and CGRP immunoreactive cells in the digestive tract of dorado (Salminus brasiliensis), Tissue and Cell, 47, 123–131, (2015).
  • Kuru, N., Çınar, K., Şenol, N., Demirbağ, E. ve Diler, D., Endocrine Cells in the gastrointestinal tract of Garra rufa, Kafkas Universitesi Veteriner Fakültesi Dergisi,16 (Suppl-B), S235-S241, (2010).
  • Kaptaner, B., Immunohistochemical distribution of insulin-, glucagon- and somatostatin containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae), European Journal of Histochemistry, 63, 2999, (2019).
  • Lin, X., Wong, P., Ou, Y., Li, J., ve Wen, J., An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos (Forsskal, 1775), Aquaculture Research, 48(4), 1–11, (2016).

Immunohistochemical investigation of some peptides in the rudd (Scardinius erythrophthalmus) stomach and intestinal regions

Year 2023, Volume: 25 Issue: 1, 203 - 214, 16.01.2023
https://doi.org/10.25092/baunfbed.1056444

Abstract

The current study aimed to determine the localization and density of some peptides found in the digestive tract mucosa of Scardinius erythrophthalmus (Rudd). In accordance with the immunohistochemical examinations, it was observed that CCK immunopositive cells were found in the parts of the intestine except for the stomach. While gastrin immunoreactive (+) cells were not observed in the stomach and middle intestine region, a small amount of these cells were detected in the anterior intestine. In the posterior intestine, a very low density of gastrin immunopositive cells were detected. It was determined that glucagon immunopositive cells were found in very small amounts in the parts of the intestine except the stomach. While low density of somatostatin immunoreactive cells were observed in the stomach region, these cells were not found in the intestinal regions. In general, it was concluded in this study that the studied peptides were found in different concentrations in different parts of the intestine.

Project Number

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References

  • Vieira-Lopes, D.A., Pinheiro, N. L., Sales, A., Ventura, A., Araújo, F. G., Gomes, I. D. ve Nascimento, A. A., Immunohistochemical study of the digestive tract of Oligosarcus hepsetus, World Journal of Gastroenterology, 19 (12), 1919-1929, (2013).
  • Lee, C. S. ve Kaestner, K. H., Development of gut endocrine cells, Journal of Clinical Endocrinology and Metabolism, 18, 453–462, (2004).
  • Şenol, N. ve Çınar, K., Immunohistochemical localization of cholecystokinin and histamine in gastrointestinal tract in flower fısh (Pseudophoxinus antalyae), Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi, 1(1-2), 26-34, (2006).
  • Şenol, N., Sazan (Cyprinus carpio) ve Sudak (Stizostedion lucioperca) balıklarında gastrointestinal kanalın histokimyasal yapısı ve bazı peptidlerin lokalizasyonu, Doktora tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Isparta, (2009).
  • Blanco, A. M., Bertucci, J. I., Valenciano, A. I., Delgado, M. J. ve Unniappan, S., Ghrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) in the intestine, and attenuates the anorectic effects of CCK, PYY and GLP-1 in goldfish (Carassius auratus), Hormones and Behaviour, 93, 62–71, (2017).
  • Cardosoa, N. N., Firmianoa, E. M. S., Gomes, I. D., Nascimentoa, A., Sales, A. ve Araúj, F. G., Histochemical and immunohistochemical study on endocrine cells (5HT, GAS, and SST) of the gastrointestinal tract of a teleost, the characin Astyanax bimaculatus, Acta Histochemica, 117, 595–604, (2015).
  • Lee, J. H., Ku, S. K. Park, K. D. ve Lee, H. S., Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch, Journal of Fish Biology, 65, 170–181, (2004).
  • Losada, A. P., Bermudez, R. ve Failde, L. D., Giancamillo, A. D., Domeneghini C. ve Quiroga, M. I., Effects of Enteromyxum scophthalmi experimental infection on the neuroendocrine system of turbot, Scophthalmus maximus (L.), Fish and Shellfish Immunology, 40 (2), 577–583, (2014).
  • Özen, M. R., Çınar, K. ve Şenol, N., Endocrine cells in the gastrointestinal tract mucosa of zander (Stizostedion lucioperca), Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16 (Suppl-B): S339-S345, (2010).
  • Chena, M., He, M., Peng, K., Liu, T., Jin, C., Cao, W., Wang L. ve Xiao, K. An immunohistochemical study of somatostatin in the stomach and the small intestine of the African ostrich (Struthio camelus), Tissue and Cell, 45, 363–366, (2013).
  • Pereira, R. T., Costa, L.S., Oliveira, I. R., Araujo, J. C., Aerts, M., Vigliano, F. A. ve Rosa, P. V., Relative distribution of gastrin-, CCK-8-, NPY- and CGRP immunoreactive cells in the digestive tract of dorado (Salminus brasiliensis), Tissue and Cell, 47, 123–131, (2015).
  • Kuru, N., Çınar, K., Şenol, N., Demirbağ, E. ve Diler, D., Endocrine Cells in the gastrointestinal tract of Garra rufa, Kafkas Universitesi Veteriner Fakültesi Dergisi,16 (Suppl-B), S235-S241, (2010).
  • Kaptaner, B., Immunohistochemical distribution of insulin-, glucagon- and somatostatin containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae), European Journal of Histochemistry, 63, 2999, (2019).
  • Lin, X., Wong, P., Ou, Y., Li, J., ve Wen, J., An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos (Forsskal, 1775), Aquaculture Research, 48(4), 1–11, (2016).
There are 14 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Nurgül Şenol 0000-0002-3090-9835

Nurevşan Deniz 0000-0003-0516-252X

Project Number yok
Publication Date January 16, 2023
Submission Date January 11, 2022
Published in Issue Year 2023 Volume: 25 Issue: 1

Cite

APA Şenol, N., & Deniz, N. (2023). Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 25(1), 203-214. https://doi.org/10.25092/baunfbed.1056444
AMA Şenol N, Deniz N. Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi. BAUN Fen. Bil. Enst. Dergisi. January 2023;25(1):203-214. doi:10.25092/baunfbed.1056444
Chicago Şenol, Nurgül, and Nurevşan Deniz. “Kızılkanat (Scardinius Erythrophthalmus) Mide Ve bağırsak bölgelerindeki Bazı Peptitlerin Immunohistokimyasal Incelenmesi”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 25, no. 1 (January 2023): 203-14. https://doi.org/10.25092/baunfbed.1056444.
EndNote Şenol N, Deniz N (January 1, 2023) Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 25 1 203–214.
IEEE N. Şenol and N. Deniz, “Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi”, BAUN Fen. Bil. Enst. Dergisi, vol. 25, no. 1, pp. 203–214, 2023, doi: 10.25092/baunfbed.1056444.
ISNAD Şenol, Nurgül - Deniz, Nurevşan. “Kızılkanat (Scardinius Erythrophthalmus) Mide Ve bağırsak bölgelerindeki Bazı Peptitlerin Immunohistokimyasal Incelenmesi”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 25/1 (January 2023), 203-214. https://doi.org/10.25092/baunfbed.1056444.
JAMA Şenol N, Deniz N. Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi. BAUN Fen. Bil. Enst. Dergisi. 2023;25:203–214.
MLA Şenol, Nurgül and Nurevşan Deniz. “Kızılkanat (Scardinius Erythrophthalmus) Mide Ve bağırsak bölgelerindeki Bazı Peptitlerin Immunohistokimyasal Incelenmesi”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 25, no. 1, 2023, pp. 203-14, doi:10.25092/baunfbed.1056444.
Vancouver Şenol N, Deniz N. Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi. BAUN Fen. Bil. Enst. Dergisi. 2023;25(1):203-14.