Kızılkanat (Scardinius erythrophthalmus) mide ve bağırsak bölgelerindeki bazı peptitlerin immunohistokimyasal incelenmesi

Yıl 2023, Cilt: 25 Sayı: 1, 203 - 214, 16.01.2023

Nurgül Şenol , Nurevşan Deniz

https://doi.org/10.25092/baunfbed.1056444

Öz

Bu çalışmada Scardinius erythrophthalmus (Kızılkanat) sindirim kanalı mukozasında bulunan bazı peptitlerin yerleşim ve yoğunluklarının belirlenmesi amaçlanmıştır. İmmunohistokimysal incelemeler doğrultusunda CCK immunoreaktif hücrelerin midede bulunmadığı ancak ilk, orta ve son bağırsakta bulunduğu gözlendi. Mide ve orta bağırsakta gastrin immunopozitif hücreler gözlenmezken, ilk bağırsakta bu hücreler tespit edildi. Son bağırsakta gastrin immunoreaktif hücrelere az sayıda rastlandı. Glukagon immunoreaktif hücrelere midede rastlanmazken, bağırsakta az miktarda saptandı. Midede somatostatin immunopozitif hücrelerin az yoğunlukta olduğu, bağırsakta ise bulunmadığı gözlendi. Sonuç olarak peptitlerin bağırsaktaki yoğunluğunun değiştiği belirlendi.

Anahtar Kelimeler

Kızılkanat, peptit, immunohistokimya, gastrointestinal kanal

Destekleyen Kurum

yok

Proje Numarası

yok

Teşekkür

yok

Immunohistochemical investigation of some peptides in the rudd (Scardinius erythrophthalmus) stomach and intestinal regions

Öz

The current study aimed to determine the localization and density of some peptides found in the digestive tract mucosa of Scardinius erythrophthalmus (Rudd). In accordance with the immunohistochemical examinations, it was observed that CCK immunopositive cells were found in the parts of the intestine except for the stomach. While gastrin immunoreactive (+) cells were not observed in the stomach and middle intestine region, a small amount of these cells were detected in the anterior intestine. In the posterior intestine, a very low density of gastrin immunopositive cells were detected. It was determined that glucagon immunopositive cells were found in very small amounts in the parts of the intestine except the stomach. While low density of somatostatin immunoreactive cells were observed in the stomach region, these cells were not found in the intestinal regions. In general, it was concluded in this study that the studied peptides were found in different concentrations in different parts of the intestine.

Anahtar Kelimeler

Rudd, peptide, immunohistochemistry, gastrointestinal tract

Proje Numarası

yok

Kaynakça

• Vieira-Lopes, D.A., Pinheiro, N. L., Sales, A., Ventura, A., Araújo, F. G., Gomes, I. D. ve Nascimento, A. A., Immunohistochemical study of the digestive tract of Oligosarcus hepsetus, World Journal of Gastroenterology, 19 (12), 1919-1929, (2013).
• Lee, C. S. ve Kaestner, K. H., Development of gut endocrine cells, Journal of Clinical Endocrinology and Metabolism, 18, 453–462, (2004).
• Şenol, N. ve Çınar, K., Immunohistochemical localization of cholecystokinin and histamine in gastrointestinal tract in flower fısh (Pseudophoxinus antalyae), Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi, 1(1-2), 26-34, (2006).
• Şenol, N., Sazan (Cyprinus carpio) ve Sudak (Stizostedion lucioperca) balıklarında gastrointestinal kanalın histokimyasal yapısı ve bazı peptidlerin lokalizasyonu, Doktora tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, Isparta, (2009).
• Blanco, A. M., Bertucci, J. I., Valenciano, A. I., Delgado, M. J. ve Unniappan, S., Ghrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) in the intestine, and attenuates the anorectic effects of CCK, PYY and GLP-1 in goldfish (Carassius auratus), Hormones and Behaviour, 93, 62–71, (2017).
• Cardosoa, N. N., Firmianoa, E. M. S., Gomes, I. D., Nascimentoa, A., Sales, A. ve Araúj, F. G., Histochemical and immunohistochemical study on endocrine cells (5HT, GAS, and SST) of the gastrointestinal tract of a teleost, the characin Astyanax bimaculatus, Acta Histochemica, 117, 595–604, (2015).
• Lee, J. H., Ku, S. K. Park, K. D. ve Lee, H. S., Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch, Journal of Fish Biology, 65, 170–181, (2004).
• Losada, A. P., Bermudez, R. ve Failde, L. D., Giancamillo, A. D., Domeneghini C. ve Quiroga, M. I., Effects of Enteromyxum scophthalmi experimental infection on the neuroendocrine system of turbot, Scophthalmus maximus (L.), Fish and Shellfish Immunology, 40 (2), 577–583, (2014).
• Özen, M. R., Çınar, K. ve Şenol, N., Endocrine cells in the gastrointestinal tract mucosa of zander (Stizostedion lucioperca), Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16 (Suppl-B): S339-S345, (2010).
• Chena, M., He, M., Peng, K., Liu, T., Jin, C., Cao, W., Wang L. ve Xiao, K. An immunohistochemical study of somatostatin in the stomach and the small intestine of the African ostrich (Struthio camelus), Tissue and Cell, 45, 363–366, (2013).
• Pereira, R. T., Costa, L.S., Oliveira, I. R., Araujo, J. C., Aerts, M., Vigliano, F. A. ve Rosa, P. V., Relative distribution of gastrin-, CCK-8-, NPY- and CGRP immunoreactive cells in the digestive tract of dorado (Salminus brasiliensis), Tissue and Cell, 47, 123–131, (2015).
• Kuru, N., Çınar, K., Şenol, N., Demirbağ, E. ve Diler, D., Endocrine Cells in the gastrointestinal tract of Garra rufa, Kafkas Universitesi Veteriner Fakültesi Dergisi,16 (Suppl-B), S235-S241, (2010).
• Kaptaner, B., Immunohistochemical distribution of insulin-, glucagon- and somatostatin containing cells in the pancreas of Lake Van fish (Alburnus tarichi Güldenstädt, 1814) (Cyprinidae), European Journal of Histochemistry, 63, 2999, (2019).
• Lin, X., Wong, P., Ou, Y., Li, J., ve Wen, J., An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos (Forsskal, 1775), Aquaculture Research, 48(4), 1–11, (2016).