Elma Kabuğu Ekstresi Temelli Organik-İnorganik Nançiçek ile İçsel Peroksidaz Benzeri ve Antimikrobiyal Aktiviteler

Abstract

Amaç: Bu çalışmada, flavonoid açısından zengin elma kabuğu özütü ve Cu2+ iyonlarının sırasıyla organik ve inorganik bileşenler olarak hareket ettiği hibrit organik-inorganik nanoçiçek (NF) sentezini geliştirdik. Metod: Elma kabuğu özütü temelli NÇ'ler (Ek-NÇ'ler) peroksidaz benzeri ve antimikrobiyal aktiviteler gösterdi. Ek-NÇ'lerin morfolojiyi (boyut ve şekil) izlemek için Taramalı Elektron Mikroskopu (SEM), eğilme ve gerilme titreşimleri için Fourier Dönüşümlü Kızılötesi Spektroskopisi (FTIR) ve Ek-NÇ'lerin kristal yapısını açıklamak için X-ışını kırınımı (XRD) ile karakterize edildi. Bulgular: Katalitik aktiviteler açısından, Ek-NÇ'ler hidrojen peroksit (H2O2) varlığında Fenton reaksiyonu yoluyla guaiacolün (2-metoksi fenol) 3,3-dimetoksi-4,4-difenokinona oksidasyonunu katalize etti. Hem Fenton reaksiyonundan hem de Ek-NÇ'lerin gözenekli morfolojisinden yararlanılarak, antimikrobiyal aktiviteleri Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) ve Candida albicans (C. albicans)'a karşı test edildi. Sonuç: Ek-NÇ'lerin H2O2 varlığında tüm mikroorganizmaların neredeyse %99'unu öldürerek büyük antimikrobiyal aktivite gösterdiğini belirtmekte fayda var. Sonuçlar oldukça ümit verici ve Ek-NÇ'lerin katalitik aktivite ve antimikrobiyal çalışmalar için ideal aday olabileceğini iddia ediyoruz.

Keywords

• Elma Kabuğu Ekstraktı
• Organik-İnorganik Nanoçiçek
• Peroksidaz Benzeri Aktivitesi
• Fenton Reaksiyonu
• Antimikrobiyal Aktivite

Ethical Statement

Conflicts of interest: No Conflict of Interest. Funding: No Funding Received. Ethical statement: This study does not require ethical approval. Author contributions: All authors read and approved the final manuscript. This study was presented as an oral presentation at the conference titled ‘Elegans International Scientific Conference Series, First International Conference on Life Science’ and its abstract was published in the conference book.

Apple peel extract based formation of organic-inorganic nanoflower with ıntrinsic peroxidase mimic and antimcrobial activities

Abstract

IPurpose: In this study, we developed synthesis of hybrid organic-inorganic nanoflower (NF) composed of flavonoid-rich apple peel extract and Cu2+ ions acted as organic and inorganic components, respectively. The apple peel extract based NFs (Ap-NFs) showed peroxidase mimic and antimicrobial activities. Method: The Ap-NFs were characterized with by Scanning Electron Microscope (SEM) for monitoring morphology (size and shape), Fourier transform Infrared Spectroscopy (FTIR) for bending and stretching vibrations and X-Ray Diffraction (XRD) for elucidation of crystal structure of Ap-NFs. Findings: In terms of the catalytic activities, Ap-NFs catalyzed oxidation of guaiacol (2-methoxy phenol) to 3,3-dimethoxy-4,4-diphenoquinone in the presence of hydrogen peroxide (H2O2) through the Fenton reaction. Benefiting from both the Fenton reaction and porous morphology of Ap-NFs, their antimicrobial activities were tested towards, Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Candida albicans (C. albicans). Conclusion: It is worthy to mention that, the Ap-NFs exhibited great antimicrobial activity in the presence of H2O2 by killing almost 99% of all microorganisms. The results are quite promising and we claim that Ap-NFs can be ideal candidate for catalytic activity and antimicrobial studies.

Keywords

• Apple Peel Extract
• Organic-Inorganic Nanoflower
• Peroxidase Mimic Activity
• Fenton Reaction
• Antimcrobial Activity

Ethical Statement

Conflicts of interest: No Conflict of Interest. Funding: No Funding Received. Ethical statement: This study does not require ethical approval. Author contributions: All authors read and approved the final manuscript. This study was presented as an oral presentation at the conference titled ‘Elegans International Scientific Conference Series, First International Conference on Life Science’ and its abstract was published in the conference book.

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