Determination of genetic relationships between some endemic Salvia species using RAPD markers

Abstract

In this study, randomly amplified polymorphic DNA RAPD analysis was used to determine genetic relationship among four endemic Salvia species. A total of 15 primers were screened, among them 7 primers produced polymorphic and reproducible bands. Eighty one DNA bands were amplified, among which 80 bands 98.7% were polymorphic. The number of bands amplified per primer varied from 7 to 14. Similarity coefficients were calculated and a dendrogram was constructed by using UPGMA algorithm. In dendrogram, the four Salvia species were divided into two major clusters. Cluster I included two subclusters, one containing all accessions of S. wiedemannii and the other one containing accessions of S. tchihatcheffii. Cluster II was also separated into two subclusters. The first subcluster contained all accessions belonging to S. crypthanta and the second subcluster contained S. cyanescens. The genetic relationships estimated by the RAPD analysis are basically in agreement with morphological data. Thus, RAPD technique is a reliable marker system that can be used to study genetic relationship in the genus Salvia

Keywords

• Salvia,Genetic relationship,RAPD,PCR,Endemic plant

Bazı endemik Salvia türleri arasındaki genetik akrabalığın RAPD belirteçleri ile saptanması

Abstract

Bu çalışmada, rastgele çoğaltılmış polimorfik DNA RAPD analizi 4 endemik Salvia türü arasındaki genetik akrabalığı belirlemek amacıyla kullanılmıştır. Toplamda 15 primer denenmiş bunlardan 7’si polimorfik ve tekrar çoğaltılabilen bantlar oluşturmuştur. Çoğaltılan 81 banttan 80’i %98.7 polimorfiktir. Primer başına çoğaltılan bant sayısı 7 ila 14 arasında değişmiştir. Benzerlik katsayısı hesaplanarak UPGMA işlemi ile dendrogram oluşturulmuştur. Bu dendrogramda 4 Salvia türü iki ana kümeye ayrılmıştır. Birinci küme iki alt kümeye ayrılarak ilki S. wiedemannii’ye ait bireyleri, diğer alt küme ise S. tchihatcheffii’ye ait bireyleri içermiştir. İkinci küme de iki alt kümeye ayrılmıştır. İlk alt küme S. crypthanta’ya ait bireyleri içerirken ikinci alt küme S. cyanescens’i içermiştir. RAPD analizi ile belirlenen bu genetik ilişki temelde morfolojik verilerle uyum göstermektedir. Dolayısıyla, RAPD tekniği Salvia cinsi içerisinde genetik akrabalık çalışmalarında kullanılabilir, güvenilir bir belirteç sistemidir

Keywords

• Salvia,genetik ilişki,RAPD,PCR,endemik bitki

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