Türkiye’de yetişen Ferula orientalis L’nin toprak üstü ve kök ekstrelerinin streptozotosin ile indüklenen diyabetik sıçanlarda erektil disfonksiyon üzerine etkisi

Abstract

Apiaceae familyasına ait Ferula L. cinsinin Türkiye’de 22 türü yetişmekte olup bu türlerin 13'ü endemiktir ve bu cins zamk-reçine bakımından zengindir. Ferula türleri Türkiye’nin çeşitli yörelerinde “çakşır, çakşırotu, helizan, çağşır, kıngor, çağ vb.” adlarıyla bilinmektedir. Türkiye’de F. orientalis “heliz” adıyla bilinir ve karminatif, sedatif, antispazmotik, laksatif, dijestif, ekspektoran, diüretik, afrodizyak, antiseptik, antihelmentik, analjezik ve stimulan olarak kullanılmaktadır. Bu çalışmanın amacı, F. orientalis ekstrelerinin corpus cavernosum (CC) üzerindeki gevşetici etkisini in vitro göstermektir. 16 adet erkek Sprague-Dawley sıçan diyabetik ve kontrol olarak iki gruba ayrıldı. Diyabet grubuna tek doz intraperitoneal enjeksiyonla streptozotosin (40 mg/kg) verilerek diyabet oluşturuldu. Organ banyosu çalışmalarında, izole korpus kavernosum dokularında asetilkoline (ACh, 1 mM), elektrik alan stimülasyonuna (EFS, 20 Hz) and sodyum nitroprusite (SNP, 0.1 μM) bağlı gevşeme cevapları alınmış, bitkinin toprak üstü ve köklerinden elde edilen sulu ekstreler ile tekrarlanmıştır. Ekstrelerin her iki grupta aktif olduğu görülmüştür. Kök ekstrelerinin % 98.12 gevşeme sağladığı bulunmuştur. Kök ekstrelerinin en iyi aktivite gösterdiği tespit edilmiştir. Bunun yanısıra, toprak üstü kısımlarına ait ekstreler en kötü aktiviteyi göstermiştir. Bu bulgulara bağlı olarak, bu türün kökleri afrodizyak potansiyel bakımından daha fazla in vivo değerlendirmeyi hak etmektedir.

Keywords

• erektil disfonksiyon
• Apiaceae
• rat
• Ferula

Effect of aerial part and root extracts from Ferula orientalis L. growing in Turkey on erectile dysfunction in streptozotocin-ınduced diabetic rats

Abstract

Ferula L. belongs to Apiaceae family and 22 species of this genus grow in Turkey, of which 13 are endemic and this genus is found to be a rich resource of gum-resin. Ferula species are known in various regions of Turkey as “çakşır, çakşırotu, helizan, çağşır, kıngor, çağ etc. ”. F. orientalis is known as “heliz” in Turkey and has been utilised as carminative, sedative, antispasmodic, laxative, digestive, expectorant, diuretic, aphrodisiac, antiseptic, anthelminitic, analgesic and stimulants. The purpose of this study is to indicate in vitro the relaxant effect of F. orientalis extracts on corpus cavernosum (CC). Total of 16 adult male Sprague-Dawley rats were levelly divided down the middle control and diabetic ingroups. Diabetes was induced by a single intraperitoneial injection of streptozotocin (40 mg/kg). The relaxation responses to acetylcholine (ACh, 1 mM), electrical field stimulation (EFS, 10Hz) sodium nitroprusside (SNP, 0.1 μM) of corpus cavernosum (CC) strips were studied after the incubation with the aerial parts and roots in organ baths. The extracts were active in both groups. It was found that root extracts yielded 98.12% relaxation. Among the extracts of roots indicated the best activity. In other respects, aerial part extracts demonstrated the worst activity. Depending upon this findings, the roots of this species deserve further in vivo assessments for its aphrodisiac potential.

Keywords

• erectile dysfunction
• Apiaceae
• rat
• Ferula

References

1. Davis, P.H. (1972). Flora of Turkey and the East Aegean Islands. Vol.4 Edinburgh: Edinburgh University Press.
2. Güner A, Aslan S, Ekim T, Vural M, Babaç MT (eds). (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi Yayınları.
3. Akalın, E., Özhatay, N. (1996). Üç yakın tıbbı cinsin ayırt edici morfolojik özellikleri: Peucedanum, Ferula, Ferulago. XI. Bitkisel İlaç Hammaddeleri Toplantısı, 22-24 Mayıs Bildiri Kitabı, Ankara, 195.
4. Heywood, V.H. (1971). The Chemistry and Biology of the Umbellifera. London: Academic press, 285-412.
5. Behçet, L., Arık M. (2013). An ethnobotanical investigation in East Anatolia (Turkey). Tr. J. Nature Sci., 2 (1), 86-110.
6. Dehpour, A.A., Ebrahimzadeh, M.A., Fazel, N.S., Mohammad, N.S. (2009). Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition. Grasas Y Aceites., 60(4), 405-412. https://doi.org/10.3989/gya.010109
7. Duke, J., Bogenschutz, M.J. (2002). Dr. Duke's phytochemical and ethnobotanical databases, Agricultural Research Service Publication.
8. Kojima, K., Isaka, K., Ondognii, P., Zevgeegiino, O., Gombosurengyin, P., Davgiin, K. (2000). Sesquiterpenoid derivatives from Ferula ferulioids. IV. Chem Pharm Bull., 48, 353-356. https://doi.org/10.1248/cpb.48.353

9. Karakaya, S., Göger, G., Bostanlik, F.D., Demirci, B., Duman, H., Kiliç, C.S. (2019). Comparison of essential oils of Ferula orientalis L., Ferulago sandrasica Peșmen & Quézel and Hippomarathrum microcarpum Petrov and their antimicrobial activity. Turk J Pharm Sci, 16(1), 69-75. https://doi.org/10.4274/tjps.77200
10. Mazzilli, R., Elia, J., Delfino, M., Benedetti, F., Scordovillo, G., Mazzilli F. (2015). Prevalence of diabetes mellitus (DM) in a population of men affected by erectile dysfunction (ED). Clin Ter., 166(5), 317-320. https://doi.org/10.7417/T.2015.1885
11. Rastrelli, G., Corona, G., Mannucci, E., Maggi, M. (2015). Vascular and chronological age in subjects with erectile dysfunction: a cross-sectional study. J Sex Med., 12(12), 2303-2312. https://doi.org/10.1111/jsm.13044
12. Johannes, C.B., Araujo, A.B., Feldman, H.A., Derby, C.A., Kleinman, K.P., Mckınlay, J.B. (2000). Incidence of erectile dysfunction in men 40 to 69 years old: longitudinal results from the Massachusetts male aging study. J Urol., 163(2), 460-463. https://doi.org/10.1016/S0022-5347(05)67900-1
13. Ruan, Y., Li, M., Wang, T., Yang, J., Rao, K., Wang, S., Yang, W., Liu, J., Ye, Z. (2016). Taurine supplementation improves erectile function in rats with streptozotocin-induced type 1 diabetes via amelioration of penile fibrosis and endothelial dysfunction. J Sex Med., 13(5), 778-785. https://doi.org/10.1016/j.jsxm.2016.02.164
14. El-Thaher, T.S., Matalka, K.Z., Taha, H.A., Badwan, A.A. (2001). Ferula harmonis `zallouh' and enhancing erectile function in rats: efficacy and toxicity study. Int J Impot Res., 13, 247-251.
15. Zanoli, P., Benelli, A., Rivasi, M., Baraldi, C., Vezzalini, F., Baraldi, M. (2003). Opposite effect of acute and subchronic treatments with Ferula hermonis on copulatory behavior of male rats. Int J Impot Res., 15, 450-455.
16. Hadidi, K.A., Aburjai, T., Battah, A.K. (2003). Acomparative study of Ferula hermonis root extracts and sildenafil on copulatory behaviour of male rats. Fitoterapia, 74, 242-246. https://doi.org/10.1016/S0367-326X(03)00032-7
17. Zanoli, P., Rivasi, M., Zavatti, M., Brusiani, F., Vezzalini, F., Baraldi, M. (2005). Activity of single components of Ferula hermonis on male rat sexual behavior. Int J Impot Res., 17, 513-518.
18. Kassis, E., Fulder, S., Khalil, K., Hadieh, B., Nahhas, F., Saad, B., Said, O. (2009). Efficacy and safety assessments of Ferula assa-foetida L., traditionally used in Greco-Arab herbal medicine for enhancing male fertility, libido and erectile function. The Open Complementary Medicine Journal, 1, 102-109. https://doi.org/10.2174/1876391X00901010102
19. Erdurak, C.S. (2003). Investigations on Ferulago isaurica Peşmen and F. syriaca Boiss. (Umbelliferae) species. PhD Thesis. Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Tandogan, Ankara, Turkey.
20. Ozturk, B., Gur, S., Coskun, M., Kosan, M., Erdurak, C.S., Hafez, G., Gonulalan, U., Cetinkaya, M. (2012). A new relaxant on human corpus cavernosum: Ferulago syriaca root extract. Afr J Pharm Pharmacol., 6(37), 2652-2656. https://doi.org/10.5897/AJPP11.642