Year 2020, Volume 13 , Issue 3, Pages 298 - 304 2020-12-15

Comparative Study of Total Phenolic Contents, Antioxidant and Antimicrobial Activities of Different Extracts of Corchorus olitorius L. Growing in North Cyprus
Kuzey Kıbrıs'ta Yetişen Corchorus olitorius L.'nin Farklı Ekstraktlarının Toplam Fenolik Madde İçeriği, Antioksidan ve Antimikrobiyal Aktivitelerinin Karşılaştırılması

İ̇lkin YÜCELŞENGÜN [1] , Ahmet GARGI [2]


Objective: In this study, antimicrobial and antioxidant activities as well as total phenolic content of dried leaf extracts of Corchorus olitorius grown in North Cyprus were determined. Methods: The total phenolic content, antioxidant and antimicrobial activity of the extracts were determined using the Folin-Ciocalteu, DPPH free radical and broth dilution methods, respectively. The antimicrobial activity of the extracts was tested against eight different microorganisms (Bacillus subtilis ATCC 6037, Listeria monocytogenes Scott A, Enterococcus faecalis ATCC 29212, Staphylococcus aureus 6538P, Bacillus cereus No 8, Escherichia coli O157:H7 ATCC 43895, Escherichia coli ATCC 1103, Salmonella Typhimurium NRRL-B-4420). Results: The highest phenolic content was obtained from ethanol extract as 24.61 mg GAE/g. Water, ethanol and methanol extracts of C. olitorius were inhibited the DPPH radical by 17.50%, 87.10% and 86.00%, respectively. MIC values of ethanol and methanol extracts of C. olitorius against test cultures were varied between 50.00% and 6.25%, while no inhibitory effect was observed for water extract. The most susceptible microorganisms to methanol extract of the plant were L. monocytogenes Scott A, E. faecalis ATCC 29212 and B. subtilis ATCC 6037 (MIC value of 6.25%). Conclusion: All these results showed that C. olitorius extracts are important sources showing antimicrobial and antioxidant properties.
Amaç: Bu çalışmada, Kuzey Kıbrıs'ta yetiştirilen Corchorus olitorius'un kurutulmuş yaprak ekstraktlarının antimikrobiyal ve antioksidan aktiviteleri ile toplam fenolik madde miktarı belirlenmiştir. Methodlar: Ekstraktların toplam fenolik madde miktarı, antioksidan ve antimikrobiyal aktivitesi sırasıyla Folin-Ciocalteu, DPPH serbest radikal ve sıvı seyreltme yöntemleri kullanılarak belirlenmiştir. Ekstraktların antimikrobiyal aktivitesi, sekiz farklı mikroorganizmaya (Bacillus subtilis ATCC 6037, Listeria monocytogenes Scott A, Enterococcus faecalis ATCC 29212, Staphylococcus aureus 6538P, Bacillus cereus No 8, Escherichia coli O157:H7 ATCC 43895, Escherichia coli ATCC 1103, Salmonella Typhimurium NRRL-B-4420) karşı test edilmiştir. Bulgular: En yüksek toplam fenolik madde miktarı etanol ekstraktından 24.61 mg GAE/g olarak belirlenmiştir. C. olitorius'un su, etanol ve metanol ekstraktları DPPH radikalini sırasıyla %17.50, %87.10 ve %86.00 oranında inhibe etmiştir. C. olitorius'un etanol ve metanol ekstraktlarının test kültürlerine karşı MIC değerleri %50.00 ile %6.25 arasında değişirken, su ekstraktının herhangi bir engelleyici etkisi gözlenmemiştir. L. monocytogenes Scott A, E. faecalis ATCC 29212 ve B. subtilis ATCC 6037 (%6.25 MIC değeri), bitkinin metanol ekstraktına karşı en duyarlı mikroorganizmalar olmuştur. Sonuç: Tüm bu sonuçlar, C. olitorius ekstraktlarının antimikrobiyal ve antioksidan özellikler gösteren önemli kaynaklar olduğunu göstermiştir.
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Primary Language en
Subjects Agricultural, Engineering
Journal Section Research Article
Authors

Orcid: 0000-0002-9445-5166
Author: İ̇lkin YÜCELŞENGÜN (Primary Author)
Institution: Ege Üniversitesi
Country: Turkey


Orcid: 0000-0003-4860-3241
Author: Ahmet GARGI
Institution: EGE UNIVERSITY
Country: Turkey


Dates

Application Date : October 28, 2020
Acceptance Date : December 11, 2020
Publication Date : December 15, 2020

APA Yücelşengün, İ , Gargı, A . (2020). Comparative Study of Total Phenolic Contents, Antioxidant and Antimicrobial Activities of Different Extracts of Corchorus olitorius L. Growing in North Cyprus . Biyolojik Çeşitlilik ve Koruma , 13 (3) , 298-304 . Retrieved from https://dergipark.org.tr/en/pub/biodicon/issue/58137/817146