A new microsporidian pathogen of the Mediterranean flour moth, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)

Year 2021, Volume: 14 Issue: 2, 277 - 285, 15.08.2021

Çağrı Bekircan Onur Tosun

https://doi.org/10.46309/biodicon.2021.966465

Abstract

In this study, a new Vairimorpha isolate was defined from Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) in Turkey (Malatya). Infection was determined in the host's hindgut, both larval and adult stages. Two types spore originated from the polymorphic sporulation was detected during the observations. While the binucleate ovocylindrical fresh spores were measured 4.38 ± 0.31 μm (5.09–3.77 μm, n = 100) in length and 2.80 ± 0.18 μm (3.21–2.43 μm, n=100) in width, the ovoid meiospores were measured as 3.50 ± 0.46 μm (4.98–2.51 μm, n = 100) in length and 2.02 ± 0.28 μm (2.94–1.50 μm, n=100) in width. The multilocus genetic analyses based on SSU rRNA and RPB1 genes were carried for determining the phylogenetic status of the current microsporidium. In ML and BI trees, the current microsporidium was separated into a different branch from V. necatrix strains within the Vairimorpha clade

Keywords

Key words: Ephestia kuehniella, Lepidoptera, RPB1, SSU rRNA, Vairimorpha

Akdeniz un güvesi, Ephestiae kuehniella (Zeller) (Lepidoptera: Pyralidae)’nin yeni bir mikrosporidian patojeni

Abstract

Bu çalışmada, Türkiye'de (Malatya) Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) konağından yeni bir Vairimorpha izolatı tanımlanmıştır. Enfeksiyon, hem larva hem de yetişkin aşamalarında konağın arka bağırsağında belirlendi. Gözlemler sırasında polimorfik sporülasyon kaynaklı iki tip spor tespit edilmiştir. Çift çekirdekli ovosilindirik taze sporlar 4.38 ± 0.31 μm (5.09–3.77 μm, n = 100) uzunluğunda ve 2.80 ± 0.18 μm (3.21–2.43 μm, n=100) genişliğinde ölçülürken, oval meiosporlar 3.50 ± olarak ölçüldü. 0,46 μm (4,98–2,51 μm, n = 100) uzunluğunda ve 2,02 ± 0,28 μm (2,94–1,50 μm, n=100) genişliğinde. SSU rRNA ve RPB1 genlerine dayalı multilokus genetik analizler, mevcut mikrosporidyumun filogenetik durumunu belirlemek için gerçekleştirilmiştir. ML ve BI ağaçlarında, mevcut microsporidium, Vairimorpha dizisi içindeki V. necatrix suşlarından farklı bir dala ayrıldı.

Keywords

Ephestia kuehniella, Lepidoptera, RPB1, SSU rRNA, Vairimorpha

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