Marul (Lactuca sativa L.) Tohumlarının Çimlenmesi Üzerinde Bazı Yabancı Otların Allelopatik Etkisi

Abstract

Yabancı otlar, kültür bitkilerinin büyüme ve gelişimini doğrudan veya dolaylı yoldan sınırlandırarak tarımsal üretimi olumsuz yönde etkileyebilir. Doğrudan etkiler arasında ışık, su, besin ve alan için rekabet öne çıkarken, dolaylı etkiler arasında allelopati önemli bir mekanizma olarak karşımıza çıkmaktadır. Tohum çimlenme aşaması, bitkilerin yaşam döngüsündeki en kritik evrelerden biri olup, allelopatik etkileşimler bu süreçte belirleyici bir rol oynayabilmektedir. Allelopati, bazı bitkiler tarafından salgılanan biyokimyasallar (allelokimyasallar) aracılığıyla diğer bitkilerin gelişimini baskılayan bir etkileşim biçimidir. Bu çalışmada, farklı yabancı ot türlerinin allelopatik etkilerinin belirlenmesi ve marul (Lactuca sativa) tohumlarının bu etkilere karşı duyarlılığının değerlendirilmesi amaçlanmıştır. Araştırma kapsamında, Amaranthus retroflexus, Sinapis arvensis ve Diplotaxis tenuifolia türlerinden elde edilen bitki materyalleri kurutulmuş, küçük parçalara ayrılmış ve distile suda 24 saat karıştırılmıştır. Elde edilen süspansiyon santrifüjlenmiş ve Whatman filtre kağıtlarından süzülerek ekstraktlar elde edilmiştir. Ekstraktlar %12,5, %25, %50 ve %100 oranlarında seyreltilerek, her birinde 10 marul tohumu bulunan petri kaplarına 6 mL uygulanmıştır. Örnekler, 18°C sıcaklık ve %70 bağıl nemde, 16 saat aydınlık ve 8 saat karanlık döngüsüne sahip iklim dolabında 6 gün süreyle inkübe edilmiştir. Çalışmada, kontrol grubu ile karşılaştırmalı olarak yabancı ot ekstraktlarının çimlenme yüzdesi ve kök uzunluğu üzerindeki etkileri değerlendirilmiştir. Elde edilen sonuçlar, A. retroflexus’un marul çimlenmesi üzerinde belirgin bir allelopatik etkiye sahip olmadığını gösterirken, S. arvensis ve D. tenuifolia türlerinin ise önemli düzeyde allelopatik etki gösterdiğini ortaya koymuştur. Bu bulgular, belirli yabancı ot türlerinin kültür bitkileri üzerindeki olası baskılayıcı etkilerini anlamak ve yabancı ot yönetimi stratejileri geliştirmek açısından önemli katkılar sağlamaktadır.

Keywords

• Allelokimyasallar
• Ekstraksiyon
• Amaranthus retroflexus
• Sinapis arvensis
• Diplotaxis tenuifolia

Allelopathic Effect of Some Weeds on Germination of Lettuce (Lactuca sativa L.) Seeds

Abstract

Weeds can limit the reproductive and survival traits of cultivated plants either directly or indirectly. Direct effects involve factors such as light, nutrients, water, and space, while indirect effects primarily involve allelopathy. Seed germination is one of the most crucial stages in the life cycle of all plants. Allelopathic effects are one of the significant factors influencing seed germination. Allelopathy occurs through allelochemicals, which are biochemical substances released by one plant that affect the growth of other plants. This study aimed to observe allelopathic effects and determine which weed species have a greater impact on the germination of a selected crop plant. Samples of Amaranthus retroflexus L., Sinapis arvensis L., and Diplotaxis tenuifolia (L.) DC were collected, dried, and ground into small pieces, which were then mixed in distilled water for 24 hours. The mixture was centrifuged, filtered using Whatman filter paper, and the extracts were obtained. The extracts were prepared at concentrations of 12.5%, 25%, 50%, and 100%.These extracts were applied at 6 ml per petri dish containing 10 lettuce seeds each. The petri dishes were kept in an incubator for 6 days at 18°C, with 70% humidity, and under a 16-hour light and 8-hour dark cycle. The effects of the various weed extracts on seed germination count and seedling length were observed and compared with control samples.The results indicated that Amaranthus retroflexus did not have a critical level of allelopathic effect, while Diplotaxis tenuifolia and Sinapis arvensis exhibited significant allelopathic abilities that were of critical importance.

Keywords

• Allelochemicals
• extracts
• Amaranthus retroflexus
• Sinapis arvensis
• Diplotaxis tenuifolia

References

1. Akın B., Akanıl Bingöl N., Leblebici S., 2017. Allelopathic effect of Lythrum salicaria L. extracts on germination and seedling growth of lettuce seeds. Akademia Disiplinlerarası Bilimsel Araştırmalar Dergisi, 3 (1), 23–30.
2. Akın B., Bingöl N., Leblebici S., 2019. Allelopathic effects of different temperatures and concentrations of Lythrum salicaria L. extracts on lettuce seed germination. Avrupa Bilim ve Teknoloji Dergisi, 17, 290–296.
3. Alam S.M., Islam E., 2002. Effects of aqueous extract of leaf, stem and root of nettleleaf goosefoot and NaCl on germination and seedling growth of rice. Pakistan Journal of Seed Technology, 1, 47–52.
4. Al-Qudah M.A., Al-Jaber H.I., Muhaidat R., Hussein E.I., Hamid A.A., Al-Smadi M.L., Abu-Orabi S.T., 2011. Chemical composition and antimicrobial activity of the essential oil from Sinapis alba L. and Sinapis arvensis L. (Brassicaceae) growing wild in Jordan. Natural Product Research, 25 (15), 1481–1490.
5. Başaran F., 2022. Weed–crop interaction: competition. Journal of Sustainable Environment, 2 (1), 9–18.
6. Baziar M.R., Farahvash F., Mirshekari B., Rashidi V., 2014. Allelopathic effect of ryegrass (Lolium persicum) and wild mustard (Sinapis arvensis) on barley. Pakistan Journal of Botany, 46 (6), 2069–2075.
7. Bendimerad N., Bendiab S.A.T., Breme K., Fernandez X., 2007. Essential oil composition of aerial parts of Sinapis arvensis L. from Algeria. Journal of Essential Oil Research, 19 (3), 206–208.
8. Bingöl Ö., Battal A., Erez M.E., Aslan A., 2022. Investigation of the allelopathic effects of lyophilized ethanol extract of Xanthoparmelia somloensis (Gyelnik) Hale lichen on tomato plant. Anatolian Journal of Botany, 6 (1), 39–43.

9. Bradow J.M., Connick W.J.Jr., 1987. Allelochemicals from Palmer amaranth (Amaranthus palmeri Wats.). Journal of Chemical Ecology, 13, 185–202.
10. Buchholtz K.P., 1967. Report of the terminology committee of the Weed Science Society of America. Weeds, 15, 388–389.
11. Cheng F., Cheng Z., 2015. Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Frontiers in Plant Science, 6, 1020.
12. Connick W.I., Brandon I.M., Legenori M.G., Vail S.L., Meneges R.M., 1987. Identification of volatile allelochemicals from Amaranthus palmeri S. Wats. Journal of Chemical Ecology, 13, 463–472.
13. Cunedioğlu T., Üremiş İ., 2018. Effects of essential oils of Rosmarinus officinalis L. and Origanum minutiflorum on germination of some weed seeds. Mustafa Kemal University Journal of Agricultural Faculty, 23 (1), 24–32.
14. De Souza M.C., De Carvalho L B., Da Costa Aguiar Alves P.L., Fidelis Giancotti P.R., 2011. Allelopathy in pigweed: a review. Communications in Plant Sciences, 1 (1), 5–12.
15. Efil F., Üremiş İ., 2019. Effects of essential oils obtained from Origanum syriacum L. and Origanum majorana L. on germination and development of some weed seeds. Turkish Journal of Weed Science, 22 (1), 25–35.
16. Fiorito S., Epifano F., Palmisano R., Genovese S., Taddeo V.A., 2017. A re-investigation of the phytochemical composition of the edible herb Amaranthus retroflexus L. Journal of Pharmaceutical and Biomedical Analysis, 143, 183–187.
17. Harvey J.A., Malcicka M., 2015. Climate change, range shifts and multitrophic interactions. In: Biodiversity in Ecosystems – Linking Structure and Function. InTech Open, 85–109 p.
18. Housny, O., 2023. Allelopathic activity of crop residues against noxious weeds: a review with particular emphasis on wheat residues. Journal of King Abdulaziz University: Meteorology, Environment & Arid Land Agriculture Sciences, 32 (1), 51–75.
19. InderjitDakshini K.M.M., Einhellig F.A., 1995. Allelopathy: organisms, processes, and applications. ACS Symposium Series 582. American Chemical Society, Washington DC, USA.abran K., Mahajan G., Sardana V., Chauhan B.S., 2015. Allelopathy for weed control in agricultural systems. Crop Protection, 72, 57–65.
20. Jin J., Koroleva O.A., Gibson T., Swanston J., Magan J., Zhang Y., Wagstaff C., 2009. Analysis of phytochemical composition and chemoprotective capacity of rocket (Eruca sativa and Diplotaxis tenuifolia) leafy salad following cultivation in different environments. Journal of Agricultural and Food Chemistry, 57 (12), 5227–5234.
21. Kaymak H.Ç., 2018. Effects of juglone and walnut leaf extract on some growth parameters and yield of radish (Raphanus sativus L.). Atatürk University Journal of Agricultural Faculty, 49 (1), 1–5.
22. Kocaçalışkan İ., 2006. Allelopathy. Bizim Büro Publishing, Kütahya, 132 p.
23. Kombıçak F.K., Arısoy G., Kuşluk B., Yılar M., 2023. Allelopathic effects of Diplotaxis tenuifolia (L.) DC. on some cultivated plants. Kırşehir Ahi Evran University Journal of Agricultural Faculty, 3 (2), 235–243.
24. Konstantinović B., Blagojević M., Samardžić N., 2014. Allelopathic effect of weed species Amaranthus retroflexus L. on maize seed germination. Herbologia, 14 (2), 123–130.
25. Menges R.M., 1987. Allelopathic effects of Palmer amaranth (Amaranthus palmeri) and other plant residues in soil. Weed Science, 35, 339–347.
26. Menges R.M., 1988. Allelopathic effect of Palmer amaranth (Amaranthus palmeri) on seedling growth. Weed Science, 36, 325–328.
27. Mlakar S.G., Jakop M., Bavec M., Bavec F., 2012. Allelopathic effects of Amaranthus retroflexus and Amaranthus cruentus extracts on garden cress germination. African Journal of Agricultural Research, 7 (10), 1492–1497.
28. Modhej A., Rafatjoo A., Behdarvandi B., 2013. Allelopathic inhibitory potential of some crop species (wheat, barley, canola, and safflower) and wild mustard (Sinapis arvensis). Annals of Biological Research, 4 (3), 195–198.
29. Öten M., Şin B., Kiremitci S., 2022. Effects of seed and leaf extracts of Sorghum bicolor on germination parameters of some plants. Journal of Agricultural Biotechnology, 3 (1), 40–52.
30. Putnam A.R., Tang C.S. (Eds.), 1986. The science of allelopathy. John Wiley & Sons, New York, 317 p.
31. Reigosa M.J., Pedrol N., González L. (Eds.), 2006. Allelopathy: a physiological process with ecological implications. Springer, Berlin, 320 p.
32. RStudio Team., 2023. RStudio: Integrated Development for R. RStudio, PBC. https://www.rstudio.com/ (Access Date: 30th March 2025).
33. Rice E.L., 1984. Allelopathy. 2nd Edition. Academic Press, Orlando, Florida, USA, 424 p.
34. Shams M., Esfahan E.Z., Ramezani M., Ghandkanlou M., 2014. Cuminum cyminum. Iranian Journal of Field Crops Research, 12 (1), 54–63.
35. Soylu S., Sertkaya E., Üremiş İ., Bozkurt İ.A., Kurt Ş., 2017. Identification and prevalence of major pathogens, pests, and weeds in lettuce (Lactuca sativa L.) production areas in Hatay Province. Mustafa Kemal University Journal of Agricultural Faculty, 22 (1), 23–33.
36. Telci İ., 2006. Essential oils and allelopathy. In: Proceedings of the Allelopathy Workshop “Allelopathy in Turkey: Past, Present, Future”, 13–15 June 2006, Yalova, 153–159 p.
37. Tursun N., Işıkber A.A., Alma M.H., Bozhüyük A.U., 2022. Inhibitory effect of oregano and laurel essential oils and their components on seed germination. Selcuk Journal of Agriculture and Food Sciences, 36 (2), 275–281.
38. Tutenocaklı T., Coşkun Y., Taş İ., Oral A., Türker G., 2022. Allelopathic effects of some essential oil components on germination and seedling growth of wheat. Current Trends in Natural Sciences, 11 (21), 513–520.
39. Uludağ A., Üremiş İ., Ruşen M., Tursun N., 2017. Possible uses of allelopathy in weed control in organic farming in Turkey. Acta Herbologica, 26 (2), 87–93.
40. Üremiş İ., Arslan M., Sangun M.K., 2009. Herbicidal potential of essential oils on the germination of some problem weeds. Asian Journal of Chemistry, 21 (4), 3199–3210.
41. Waller G.R., 1987. Allelochemicals: role in agriculture and forestry. ACS Symposium Series, 330 p.
42. Weston L.A., 2005. History and current trends in the use of allelopathy for weed management. HortTechnology, 15 (3):, 529–534.
43. Xuan T.D., Tsuzuki E., 2001. Effects of application of alfalfa pellet on germination and growth of weeds. Journal of Crop Production, 4, 303–312.
44. Xuan T.D., Tsuzuki E., 2002. Varietal difference in allelopathic potential of alfalfa. Journal of Agronomy and Crop Science, 188 (1), 2–7.
45. Xuan T.D., Tsuzuki E., 2004. Allelopathic plants: buckwheat. Allelopathy Journal, 13, 137–148.
46. Yarnia M., İkincikarakaya S.Ü., Rezaei F., Khawar K M., 2011. Effects of rye residues on soil seed bank and development of redroot pigweed (Amaranthus retroflexus L.). Iğdır University Journal of Institute of Science and Technology, 1 (2), 91–96.
47. Yılar M., Bayar Y., Onaran A., 2019. Chemical composition and allelopathic effect of Origanum onites L. essential oil. Plant Protection Bulletin, 59 (3), 71–78.