Bazı Trichoderma türlerin antepfıstığında Rhizoctonia Solani’nın neden olduğu kök çürüklüğü hastalığına karşı etkinliğinin belirlenmesi
Year 2026,
Volume: 66 Issue: 1
,
87
-
95
,
31.03.2026
Sena Nur Seba
,
Mehmet Hadi Aydın
Abstract
Tarımsal üretimde kullanılan birçok kimyasal ilaçın yol açtığı çevresel kirlilik, kalıntı sorunu ve toprak patojenleriyle mücadelenin zor olması nedeniyle, bitki hastalıklarıyla mücadelede alternatif yöntemler araştırılmaktadır. Biyolojik mücadele bu alternatif yöntemlerin en önemlilerinden biridir. Trichoderma Pers.türleri biyolojik mücadelede en fazla kullanılan antagonistlerdir. Bu çalışmada, bazı Trichoderma türlerinin Rhizoctonia solani Kühn.’e karşı uçucu metabolitler yoluyla, ikili kültür yöntemiyle ve yapay bulaştırılmış saksı toprağında etkinlikleri araştırılmıştır. Çalışma sonucunda uçucu metabolitler yoluyla Trichoderma sp.FT1 izolatının %33,88 oranıyla patojeni en fazla engelleyen izolat olduğu belirlenmiştir. İkili kültür yöntemiyle yapılan çalışmada ise Trichoderma harzianum TUZ16 izolatının patojeni %70,97 oranında engelleyerek güçlü hiperparazit özelliği gösterdiği tespit edilmiştir. Uygulama yapılmış antepfıstığı tohumlarıyla kurulan saksı denemesinde Trichoderma harzianum TUZ16, Trichoderma virens İB1 Trichoderma viride VG18 ve Trichoderma sp. FT1antagonistlerin sırasıyla, %66,66, %60,00, %56,66 ve %43,33 oranında patojeni baskıladığı belirlenmiştir.
Project Number
2023-SİÜFEB-007
References
-
Abbas A., Jiang D., Fu Y., 2017. Trichoderma spp. as antagonist of Rhizoctonia solani. Journal Plant Pathology Microbiology, 8 (1), 402-403.
-
Abbott W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18, 265-267.
-
Abu-Qaoud H., 2005. Effect of scarification, gibberellic acid and stratification on seed germination of three Pistacia species. An-Najah Univ. Journal for Research, 21, 1-11.
-
Ak B.E., Özgüven A.I., Nikpeyma Y., 1995. The effect of GA3 applications on pistachio nut seed germination and seedling growth. Acta Horticulturae, 419, 115-120.
-
Aydın M.H., Turhan G., 2009. Rhizoctonia solani’nin fungal antagonistlerinin belirlenmesi üzerinde araştırmalar. Anadolu Journal of Aegean Agricultural Research Institute, 19 (2), 49-72.
-
Aydın M.H., Turhan G., Göre E., 2011. Patates yumrularında Rhizoctonia solani Kühn sklerotlarının canlılığı ve oluşumu üzerine bazı antagonistlerin etkinliğinin belirlenmesi. Anadolu Journal of Aegean Agricultural Research Institute, 21 (2), 29-38.
-
Aydın M.H., 2015. Bitki fungal hastalıklarıyla biyolojik savaşta Trichoderma’lar. Türkiye Tarımsal Araştırmalar Dergisi, 2 (2), 135-148.
-
Aydın M.H., Turhan G., 2017. A novel technique for the recovery, isolation and preliminary evaluation of Rhizoctonia solani mycoparasites from soil. The journal of Turkish phytopathology, 46 (1), 43-51.
-
Aydın M.H., İnal B., 2019. Genetic characterization and virulence of Fusarium spp. isolated from chickpea, Cellular and Molecular Biology (Noisy le Grand), 65 (1), 56-60.
-
Aydın M.H., Ünal F., 2021. Siirt ilindeki fıstık (Pistacia vera L.) fidanlarından izole edilen Rhizoctonia solani Kühn izolatlarının anastomoz grupları ve patojenitesi. Türkiye Tarımsal Araştırmalar Dergisi, 8 (1), 18-26.
-
Aydın M.H., 2022. Rhizoctonia solani and its biological control. Turkish Journal of Agricultural Research, 9 (1), 118-135.
-
Aydın M.H., İnal B., Uzun T., Aydın Y., Kayalar B., 2023. Antep fıstığı (Pistacia vera L.) bahçelerinde kök çürüklüğü ve solgunluğa neden olan fungal etmenlerin araştırılması, Siirt İli Lokasyonu, Türkiye, Türkiye Tarımsal Araştırmalar Dergisi, 10 (3), 308-319.
-
Aydın M.H., Canpolat S., Uzun T., 2024. Some soilborne pathogens causing root rot and wilting of pistachio trees in southeastern Turkey. Canadian Journal of Plant Pathology, 46 (4), 395-409. doi: 10.1080/07060661.2023.2282549.
-
Bains P.S., Bennypaul H.S., Lynch D.R., Kwachuk L.M., Schaupmeyer C.A., 2002. Rhizoctonia disease of potatoes (Rhizoctonia solani): fungicidal efficacy and cultivar susceptibility. American Journal of Potato Research, 79 (2), 99-106.
-
Baker K.F., Cook R.J., 1974. Biological control of plant pathogens. W.H. Freeman and Company, San Francisco, 433 p.
-
Bell D.K., Weels H.D., Markham C.R., 1982. In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, 72, 379-382.
-
Biçici M., Erkılıç A., 1986. Patateste siyah kabukluk ve gövde kanseri yapan Rhizoctonia solani Kühn’nin entegre kontrolü. Doğa, 10 (2), 149-173.
-
Bisset J., 1991. Revision of the genus Trichoderma II, infrageneric classification. Canadian Journal of Botany, 69, 2357-2372.
-
Boosalis M.G., Scharen A.L., 1959. Methods for microscopic detection of Aphanomyces euteiches and Rhizoctonia solani and for isolation of Rhizoctonia solani associated with plant debris, Phytopathology, 49 (4), 192-198.
-
Bora T., Özaktan H., 1998. Bitki hastalıklarıyla biyolojik savaş. Prizma Matbaası, s. 205, Bornova-İzmir.
-
Cai F., Druzhinina I.S., 2021. In honor of John Bissett: authoritative guidelines on molecular identification of Trichoderma. Fungal Diversity, 107, 1–69. https://doi.org/10.1007/s13225-020-00464-4
-
Carling D.E., Rothrock C.S., Macnish G.C., Brainard M.W., Winters S.W., 1994. Characterization of anastomosis group 11 (AG 11) of Rhizoctonia solani. Phytopathology, 84 (12), 1387-1393.
-
Chen S.F., Morgan D.P., Michailides T.J., 2013. First report of Phoma fungicola associated with stem canker and fruit blight of pistachio in Arizona. Plant Pathology, 95 (2), 451.
-
Chitzanidis A., 1995. Pistachio diseases in Greece. In: First International Symposium on Pistachio Nut. Kaska N., Kuden A.B., Ferguson L., Michailides T., (Eds.), Acta Horticulturae, 419, 345-348.
-
Clarkson J.D.S., Cook R.J., 1983. Effect of sharp eyespot on yield loss in winter wheat. Plant Pathology, 32 (4), 421-428.
-
Delen N., 2020. Bitki patojenlerinde fungisitlere duyarlılık azalışı. Nobel Yayınevi, s.133, Ankara.
-
Dennis C., Webster J., 1971. Antagonistic properties of species groups of Trichoderma II. Production of volatile antibiotics. Transactions of the British Mycological Society, 57, 41-48.
-
Eskalen A., Küsek M., Danıştı L., Karadağ S., 2001. Fungal diseases in pistachio trees in East-Mediterranean and Southeast Anatolian regions. Cahiers Options Mediterraneennes, 56, 261-264.
-
Ganeshamoorthi P., Dubey S.C., 2015. Morphological and pathogenic variability of R. solani isolates associated with wet root rot of chickpea in India. Legume Research, 38 (3), 389-395.
-
Kredics L., Antal Z., Manczinger L., Szekeres A., Kevei F., Nagy E., 2003. Trichderma strains with biocontrol potential. Food Technology and Biotechnology, 41 (1), 37-42.
-
Mahalakshmi P., Raja Y.I., 2013. Biocontrol potential of Trichoderma species against wilt disease of carnation (Dianthus caryophyllus L.) caused by Fusarium oxysporum f.sp. dianthi. Journal of Biopesticides, 6 (1), 32-36.
-
Michailides T.J., Morgan D.P., Doster M.A., Kaska N., Kuden A.B., Ferguson L., Michailides T., 1995. Diseases of pistachio in California and their significance. Acta Horticulturae, 419 (56), 337-344.
-
Mohammadi M., Banihashemi M., Hedjaroude G.A., Rahimian H., 2003. Genetic diversity among Iranian isolates of Rhizoctonia solani Kühn anastomosis group 1 subgroups based on isozyme analysis and total soluble protein pattern. Journal of Phytopatology, 151 (3), 162-170.
-
Samuels G.J., Petrini O., Kuhls K., Lieckfeldt E., Kubicek P., 1998. The Hypocrea schweinitzii I complex and Trichoderma sect. Longibrachiatum. Studies in Mycology, 41, 1-54.
-
Samuels G.J., 2006. Trichoderma: systematics, the sexual state, and ecology. Phytopathology, 96 (2), 195-206.
-
Samuels G.J., Chaverri P., Farr D.F., McCray E.B., 2012. Trichoderma Online, Systematic Mycology and Microbiology Laboratory. (Washington DC: USDA-ARS). Available: http://nt.ars-grin.gov/taxadescriptions/keys/TrichodermaIndex.cfm
-
Stefany C., Petrillo C., Donadio G., Piaz F.D., Cimmino A., Masi M., Evidente A., Isticato R., 2021. Plant growth promotion function of Bacillus sp. strains isolated from Salt-Pan rhizosphere and their biocontrol potential against Macrophomina phaseolina. International Journal of Molecular Sciences, 22 (7), 3324.
-
Townsend G.R., Heuberger J.W., 1943. Methods for estimating losses caused by diseases in fungicide experiments. The Plant Disease Reporter, 27, 340-343.
-
Trillas M.I., Casanova E., Cotxarrera L., Ordovàs J., Borrero C., Avilès M., 2006. Composts from agricultural waste and the Trichoderma asperellum strain T-34 suppress Rhizoctonia solani in cucumber seedlings. Biological Control, 39 (1), 32-38.
-
Tsror L., 2010. Biology, epidemiology and management of Rhizoctonia solani on potato. Journal of Phytopathology, 158 (10), 649-658.
-
Turhan G., 1990. Further hyperparasites of Rhizoctonia solani Kühn as promising candidates for biological control. Zeitschrift für Pflanzenkrankheiten and Pflanzenschuts, 97, 208-215.
-
Turhan G., 1992. Unterdrückrung des Rhizoctonia-Befalls durch einen neuen Mykoparasiten, Stachybotrys elegans, 48. Deutsche Pflanzen schuztagung, Göttingen (FRG).
-
Türkölmez Ş., Çiftçi O., Derviş S., Ulubaş Serçe Ç., 2015. First report of Phytophthora palmivora causing crown and root rot of pistachio trees in Turkey. Plant Disease, 99 (12), 1866.
-
Vincent J.M., 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159, 850.
-
Whipps J.M., Davies K.G., 2000. Biocontrol of plant pathogens and nematods by microorganisms. In: Measures of success in biological control. Guit G., Wratten S.D., (Eds.), Kluwer, Dordrecht, pp. 231-269.
Determination of the effect of Trichoderma Pers. on root rot caused by Rhizoctonia Solani Kühn. in pistachios
Year 2026,
Volume: 66 Issue: 1
,
87
-
95
,
31.03.2026
Sena Nur Seba
,
Mehmet Hadi Aydın
Abstract
Due to reasons such as environmental pollution caused by chemical pesticides used in agriculture, residue problems and the difficulty of chemical control of soil pathogens, alternative methods are being investigated to control some plant diseases. Biological control is a method developed as an alternative to chemical control. Trichoderma species are known to be the most used antagonists in biological control. This study investigated the effectiveness of some Trichoderma species against Rhizoctonia solani through volatile metabolites, using a dual culture method and in artificially contaminated potting soil in 2022-2023. The study showed that Trichoderma sp. FT1 isolate suppressed the pathogen by volatile metabolites with a rate of 33.88%. In the dual culture method, the Trichoderma harzianum TUZ16 isolate demonstrated strong hyperparasitic properties, inhibiting the pathogen by 70.97%. It was determined that the antagonists T. harzianum TUZ16, Trichoderma virens İB1, Trichoderma viride VG18, and Trichoderma sp. FT1 suppressed the pathogen in the plant by 66.66%, 60.00%, 56.66%, and 43.33%, respectively in pot study.
Ethical Statement
The article meets all applicable standards of ethics in experimentation and research, and there are no requirements for duplicate publication, dishonesty, plagiarism or animal or human experimentation.
Supporting Institution
Siirt University Scientific Research Projects Coordination Unit (BAP)
Project Number
2023-SİÜFEB-007
Thanks
Thanks to Siirt University Scientific Research Projects Coordination Unit (BAP)
References
-
Abbas A., Jiang D., Fu Y., 2017. Trichoderma spp. as antagonist of Rhizoctonia solani. Journal Plant Pathology Microbiology, 8 (1), 402-403.
-
Abbott W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18, 265-267.
-
Abu-Qaoud H., 2005. Effect of scarification, gibberellic acid and stratification on seed germination of three Pistacia species. An-Najah Univ. Journal for Research, 21, 1-11.
-
Ak B.E., Özgüven A.I., Nikpeyma Y., 1995. The effect of GA3 applications on pistachio nut seed germination and seedling growth. Acta Horticulturae, 419, 115-120.
-
Aydın M.H., Turhan G., 2009. Rhizoctonia solani’nin fungal antagonistlerinin belirlenmesi üzerinde araştırmalar. Anadolu Journal of Aegean Agricultural Research Institute, 19 (2), 49-72.
-
Aydın M.H., Turhan G., Göre E., 2011. Patates yumrularında Rhizoctonia solani Kühn sklerotlarının canlılığı ve oluşumu üzerine bazı antagonistlerin etkinliğinin belirlenmesi. Anadolu Journal of Aegean Agricultural Research Institute, 21 (2), 29-38.
-
Aydın M.H., 2015. Bitki fungal hastalıklarıyla biyolojik savaşta Trichoderma’lar. Türkiye Tarımsal Araştırmalar Dergisi, 2 (2), 135-148.
-
Aydın M.H., Turhan G., 2017. A novel technique for the recovery, isolation and preliminary evaluation of Rhizoctonia solani mycoparasites from soil. The journal of Turkish phytopathology, 46 (1), 43-51.
-
Aydın M.H., İnal B., 2019. Genetic characterization and virulence of Fusarium spp. isolated from chickpea, Cellular and Molecular Biology (Noisy le Grand), 65 (1), 56-60.
-
Aydın M.H., Ünal F., 2021. Siirt ilindeki fıstık (Pistacia vera L.) fidanlarından izole edilen Rhizoctonia solani Kühn izolatlarının anastomoz grupları ve patojenitesi. Türkiye Tarımsal Araştırmalar Dergisi, 8 (1), 18-26.
-
Aydın M.H., 2022. Rhizoctonia solani and its biological control. Turkish Journal of Agricultural Research, 9 (1), 118-135.
-
Aydın M.H., İnal B., Uzun T., Aydın Y., Kayalar B., 2023. Antep fıstığı (Pistacia vera L.) bahçelerinde kök çürüklüğü ve solgunluğa neden olan fungal etmenlerin araştırılması, Siirt İli Lokasyonu, Türkiye, Türkiye Tarımsal Araştırmalar Dergisi, 10 (3), 308-319.
-
Aydın M.H., Canpolat S., Uzun T., 2024. Some soilborne pathogens causing root rot and wilting of pistachio trees in southeastern Turkey. Canadian Journal of Plant Pathology, 46 (4), 395-409. doi: 10.1080/07060661.2023.2282549.
-
Bains P.S., Bennypaul H.S., Lynch D.R., Kwachuk L.M., Schaupmeyer C.A., 2002. Rhizoctonia disease of potatoes (Rhizoctonia solani): fungicidal efficacy and cultivar susceptibility. American Journal of Potato Research, 79 (2), 99-106.
-
Baker K.F., Cook R.J., 1974. Biological control of plant pathogens. W.H. Freeman and Company, San Francisco, 433 p.
-
Bell D.K., Weels H.D., Markham C.R., 1982. In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, 72, 379-382.
-
Biçici M., Erkılıç A., 1986. Patateste siyah kabukluk ve gövde kanseri yapan Rhizoctonia solani Kühn’nin entegre kontrolü. Doğa, 10 (2), 149-173.
-
Bisset J., 1991. Revision of the genus Trichoderma II, infrageneric classification. Canadian Journal of Botany, 69, 2357-2372.
-
Boosalis M.G., Scharen A.L., 1959. Methods for microscopic detection of Aphanomyces euteiches and Rhizoctonia solani and for isolation of Rhizoctonia solani associated with plant debris, Phytopathology, 49 (4), 192-198.
-
Bora T., Özaktan H., 1998. Bitki hastalıklarıyla biyolojik savaş. Prizma Matbaası, s. 205, Bornova-İzmir.
-
Cai F., Druzhinina I.S., 2021. In honor of John Bissett: authoritative guidelines on molecular identification of Trichoderma. Fungal Diversity, 107, 1–69. https://doi.org/10.1007/s13225-020-00464-4
-
Carling D.E., Rothrock C.S., Macnish G.C., Brainard M.W., Winters S.W., 1994. Characterization of anastomosis group 11 (AG 11) of Rhizoctonia solani. Phytopathology, 84 (12), 1387-1393.
-
Chen S.F., Morgan D.P., Michailides T.J., 2013. First report of Phoma fungicola associated with stem canker and fruit blight of pistachio in Arizona. Plant Pathology, 95 (2), 451.
-
Chitzanidis A., 1995. Pistachio diseases in Greece. In: First International Symposium on Pistachio Nut. Kaska N., Kuden A.B., Ferguson L., Michailides T., (Eds.), Acta Horticulturae, 419, 345-348.
-
Clarkson J.D.S., Cook R.J., 1983. Effect of sharp eyespot on yield loss in winter wheat. Plant Pathology, 32 (4), 421-428.
-
Delen N., 2020. Bitki patojenlerinde fungisitlere duyarlılık azalışı. Nobel Yayınevi, s.133, Ankara.
-
Dennis C., Webster J., 1971. Antagonistic properties of species groups of Trichoderma II. Production of volatile antibiotics. Transactions of the British Mycological Society, 57, 41-48.
-
Eskalen A., Küsek M., Danıştı L., Karadağ S., 2001. Fungal diseases in pistachio trees in East-Mediterranean and Southeast Anatolian regions. Cahiers Options Mediterraneennes, 56, 261-264.
-
Ganeshamoorthi P., Dubey S.C., 2015. Morphological and pathogenic variability of R. solani isolates associated with wet root rot of chickpea in India. Legume Research, 38 (3), 389-395.
-
Kredics L., Antal Z., Manczinger L., Szekeres A., Kevei F., Nagy E., 2003. Trichderma strains with biocontrol potential. Food Technology and Biotechnology, 41 (1), 37-42.
-
Mahalakshmi P., Raja Y.I., 2013. Biocontrol potential of Trichoderma species against wilt disease of carnation (Dianthus caryophyllus L.) caused by Fusarium oxysporum f.sp. dianthi. Journal of Biopesticides, 6 (1), 32-36.
-
Michailides T.J., Morgan D.P., Doster M.A., Kaska N., Kuden A.B., Ferguson L., Michailides T., 1995. Diseases of pistachio in California and their significance. Acta Horticulturae, 419 (56), 337-344.
-
Mohammadi M., Banihashemi M., Hedjaroude G.A., Rahimian H., 2003. Genetic diversity among Iranian isolates of Rhizoctonia solani Kühn anastomosis group 1 subgroups based on isozyme analysis and total soluble protein pattern. Journal of Phytopatology, 151 (3), 162-170.
-
Samuels G.J., Petrini O., Kuhls K., Lieckfeldt E., Kubicek P., 1998. The Hypocrea schweinitzii I complex and Trichoderma sect. Longibrachiatum. Studies in Mycology, 41, 1-54.
-
Samuels G.J., 2006. Trichoderma: systematics, the sexual state, and ecology. Phytopathology, 96 (2), 195-206.
-
Samuels G.J., Chaverri P., Farr D.F., McCray E.B., 2012. Trichoderma Online, Systematic Mycology and Microbiology Laboratory. (Washington DC: USDA-ARS). Available: http://nt.ars-grin.gov/taxadescriptions/keys/TrichodermaIndex.cfm
-
Stefany C., Petrillo C., Donadio G., Piaz F.D., Cimmino A., Masi M., Evidente A., Isticato R., 2021. Plant growth promotion function of Bacillus sp. strains isolated from Salt-Pan rhizosphere and their biocontrol potential against Macrophomina phaseolina. International Journal of Molecular Sciences, 22 (7), 3324.
-
Townsend G.R., Heuberger J.W., 1943. Methods for estimating losses caused by diseases in fungicide experiments. The Plant Disease Reporter, 27, 340-343.
-
Trillas M.I., Casanova E., Cotxarrera L., Ordovàs J., Borrero C., Avilès M., 2006. Composts from agricultural waste and the Trichoderma asperellum strain T-34 suppress Rhizoctonia solani in cucumber seedlings. Biological Control, 39 (1), 32-38.
-
Tsror L., 2010. Biology, epidemiology and management of Rhizoctonia solani on potato. Journal of Phytopathology, 158 (10), 649-658.
-
Turhan G., 1990. Further hyperparasites of Rhizoctonia solani Kühn as promising candidates for biological control. Zeitschrift für Pflanzenkrankheiten and Pflanzenschuts, 97, 208-215.
-
Turhan G., 1992. Unterdrückrung des Rhizoctonia-Befalls durch einen neuen Mykoparasiten, Stachybotrys elegans, 48. Deutsche Pflanzen schuztagung, Göttingen (FRG).
-
Türkölmez Ş., Çiftçi O., Derviş S., Ulubaş Serçe Ç., 2015. First report of Phytophthora palmivora causing crown and root rot of pistachio trees in Turkey. Plant Disease, 99 (12), 1866.
-
Vincent J.M., 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159, 850.
-
Whipps J.M., Davies K.G., 2000. Biocontrol of plant pathogens and nematods by microorganisms. In: Measures of success in biological control. Guit G., Wratten S.D., (Eds.), Kluwer, Dordrecht, pp. 231-269.