Van Balığında (Alburnus tarichi Guldenstadt, 1814) Üreme Göçü Sırasında Oksidatif Stresin Araştırılması

Year 2019, , 90 - 97, 12.03.2019

Ahmet Regaib Oğuz , Necati Özok , Aso Omar Zehra Alkan , Ayşe Nur Kıraççakalı

https://doi.org/10.17798/bitlisfen.512275

Abstract

Van Balığı, Türkiye’nin en büyük gölüne
endemik anadrom bir türdür. Balıklar, her sene üremesini gerçekleştirmek için
alkalin Van Gölünden göle dökülen tatlı sulara göç ederler. Balık bu göç
sırasında açlık, tuz ve pH adaptasyonu gibi farklı stres faktörlerine maruz
kalır. Bu çalışmada, Van Balığının üreme göçü öncesi ve sonrasında plazma, solungaç,
karaciğer, beyin ve kas gibi dokularindaki total oksidan ve antioksidan, lipid
peroksidasyon ve DNA hasarları karşılaştırıldı. Total oksidan durum (TOS) üreme
öncesi gölden yakalanan balıkların karaciğer ve böbrek dokularında, total
antioksidan durum (TAS) ise akarsudan yakalanan balıklarda karaciğerde, gölden
yakalanan balıkların ise beyin ve böbrek dokularında yüksek olduğu gözlendi
(P<0.05). Malondialdehit (MDA) seviyelerinin akarsudan örneklenen balıkların
karaciğerinde gölden örneklenen balıklarda ise böbreklerinde daha fazla olduğu
belirlendi (P<0.05). DNA hasarınin ise sadece tatlı sudan örneklenen
balıkların karaciğerinde arttığı belirlendi. Sonuç olarak, Van Balığında üreme
göçü sırasında karşılaşılan stres etkilerine karşı organların gösterdiği
tepkiler belirlendi. 

Keywords

Van Balığı, Oksidatif Stres, Lipid Peroksidasyon, DNA Hasarı

Investigation of Oxidative Stress in Van Fish (Alburnus tarichi Guldenstadt, 1814) During Reproductive Migration

Abstract

Van Fish is an anadromous species of endemic to
Turkey's largest lake. The fish migrate to the freshwater pouring from the
alkaline Lake Van to the lake for the reproduction every year. Fish are exposed
to different stress factors such as fasting, salt and pH adaptation during
migration. In this study, total oxidant and antioxidant, lipid peroxidation and
DNA damages in tissues such as gill, liver, plasma, brain and muscle were
compared before and after reproductive migration. Total oxidant status (TOS) of
the fish caught in the lake before reproduction, liver and kidney tissues,
total antioxidant status (TAS), fish liver caught from freshwater, fish from
the lake were observed to be high in the brain and kidney tissues (P <0.05).
Malondialdehyde (MDA) levels were found to be higher in the liver and kidney of
the fish sampled from the freshwater and lake, respectively. (P <0.05). DNA
damage was found only in the liver of fish sampled from freshwater. As a result,
the reactions of the organs against stress effects encountered during
reproduction migration were determined.

Keywords

Van Fish, Oxidative stress, Lipid peroxidation, DNA damage

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