Streptozotosin kaynaklı diyabetik sıçanlarda Arum rupicola'nın in vivo antioksidan potansiyeli

Year 2019, , 866 - 874, 30.09.2019

Necati Özok , İnan Güneş

https://doi.org/10.17798/bitlisfen.547871

Abstract

Diabetes mellitus, dünya popülasyonunun
azımsanmayacak bir kesimini etkileyen metabolik bir hastalıktır. Arum rupicola, Doğu ve Güneydoğu Anadolu’da,
halk hekimliğinde diyabetin tedavisi için kullanılmaktadır. Bu çalışma, Arum rupicola’nın streptozotosin (STZ)
ile oluşturulan diyabetik sıçanlarda toprak üstü kök ve gövde liyoflize
ekstresinin oksidatif stres belirteçleri üzerine etkisini değerlendirmek için
tasarlanmıştır. Çalışmanın deneysel modellemesine göre, toplam 36 adet erkek
sıçan rastgele altı gruba ayrıldı. İlk grup, normal kontrol (NK), ikinci grup
streptozotosin (STZ) deneysel diyabet oluşturuldu (DK). Üçüncü grup, standart
drug- akarboz (DAK) deneme süresince verildi. Dört, beş ve altıncı gruplardaki sıçanlar
STZ ile diyabet oluşturulduktan sonra sırasıyla 100, 200 ve 400 mg/kg (DAR 100,
DAR 200, DAR 400 mg/kg.) doz aralığında Arum
rupicola liyoflize ekstresi gavaj yolu sıçanlara verildi.

Bu çalışmada Antioksidan savunma sistemi
unsurlarından olan süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx),
katalaz (CAT) aktivitesi ile non-enzimatik antioksidan olan glutatyon (GSH)'un
düzeylerinin ölçümleri gerçekleştirildi. Ayrıca, lipit peroksidasyonun (LPO)
son ürünü malondialdehit (MDA) içeriği ölçüldü. Diyabetik gruplarda, uygulama
dozlarının tümünde SOD, GPx ve CAT ve GSH düzeylerindeki azalışlar normal
kontrol (NK) grubuna göre anlamlı bulundu. Ancak eritrosit ve karaciğer
dokularında lipit peroksidasyonu sonucu oluşan MDA'in düzeyinde görülen azalma
normal kontrol ve diyabetik kontrol gruplarına göre anlamlı bulundu (p≤0.05). Diğer
yandan, beyin dokusu DAR3 grubunda ve böbrek dokusu DAR1, DAR2, DAR3
gruplarında artan MDA düzeyleri istatiksel olarak anlamlı bulunmuştur. Bitki
ekstresi uygulanan diyabetik gruplarda, farklı dokularda belirlenen değişken
SOD, GPx ve CAT aktivite ve GSH’un ölçümleri istatistiksel olarak anlamlı
bulundu (p≤0.05).

Sonuç olarak Arum rupicola, diyabetik sıçanlarda oksidatif strese karşı
uygulanan bitki ekstresinin doz aralığına göre dokudan-dokuya farklılık
gösteren antioksidatif etkiye sahip olduğu söylenebilir.

Keywords

Arum rupicola, Antioksidan, Oksidatif stres

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