New Experimental Approaches to Sand Hardening by Microbial Biocalcification

Year 2020, , 390 - 401, 13.03.2020

Alpcan Arıç İrem Deniz , Tuğba Keskin Gündoğdu

https://doi.org/10.17798/bitlisfen.570061

Abstract

In
recent years there has been a rapid growth in the construction sector in order
to meet the need for housing with the increase in population. This growth led
to the rapid development of the cement industry. In the process of increasing
industrialization, biotechnological processes are becoming more important. The
most important alternative of the cement industry is the microbial
bio-calcification processes. In this process, cement-like structures are
produced by bacteria at room temperature conditions. Microbial
bio-calcification processes have attracted the interest of researchers
especially in the process of self-healing in recent years as an alternative and
nature-friendly solution to the cement processes that are being produced at
high temperatures. This study presents a comparative study for sand hardening
by microbial bio-calcification process. Different production surfaces was used
for sand hardening with Sporosarcina
pasteurii such as agar plates, filter paper and polyurethane support
materials. The effect of different CaCl₂ concentrations (25 mM, 50
mM and 100 mM) and sand thickness (1mm, 5mm and 10 mm) was also tested. CaCO₃
was determined by FTIR and measured by chemical analysis. In addition, the
hardness and integrity of the samples were observed. Agar and polyurethane
support materials were found to be more effective in terms of support material
for sand hardening. Increased thickness reduced the hardness and 50 mM CaCl₂
concentration was found to be optimum for these types of processes. This study
shows the effects of sand hardness on innovative, environmentally friendly  and biotechnological approach optimization.

Keywords

Bio-calcification, Bio-cement, Agar, Polyurethane, Filter paper

Supporting Institution

TUBİTAK

Project Number

2209A

Thanks

The authors wish to thank TUBITAK for financial support under 2209A programme.

New Experimental Approaches to Sand Hardening by Microbial Biocalcification

Abstract

Son
yıllarda nüfustaki artışla birlikte barınma ihtiyacının giderilmesi için inşaat
sektöründe hızlı bir büyüme olmuştur. Bu büyüme beraberinde çimento
endüstrisinde hızlı gelişimi getirmiştir. Artan endüstrileşme sürecinde
biyoteknolojik süreçler her geçen gün daha fazla önem kazanmaktadır. Çimento
endüstrisinin en önemli alternatifi mikrobiyal biyokalsifikasyon prosesleridir.
Bu proseste çimento benzeri yapılar oda sıcaklığı koşullarında bakteriler
tarafından üretilmektedirler. Mikrobiyal biyo-kalsifikasyon prosesleri yüksek
sıcaklıklarda üretimi gerçekleşen çimento proseslerine alternatif ve doğa dostu
bir çözüm olarak son yıllarda araştırmacıların ilgisini özellikle kendi kendine
iyileşme sürecinde çekmiştir. Bu çalışma kapsamında mikrobiyal biyo-kalsifikasyon
işlemiyle kum sertleşmesinde farklı üretim yüzeyleri karşılaştırılmıştır. Agar,
filtre kağıdı ve poliüretan destek malzemeleri kullanılarak Sporosarcina pasteurii ile kum
sertleştirmesi proses verimleri incelenmiştir. Farklı CaCl₂
konsantrasyonlarının (25 mM, 50 mM ve 100 mM) ve kum kalınlığının (1 mm, 5 mm
ve 10 mm) etkisi de test edilmiştir. CaCO₃ varlığı FTIR testleri ile
belirlenmiş ve konsantrasyon karşılaştırmaları kimyasal yöntemlerle
yapılmıştır. Ek olarak, numunelerin sertliği ve bütünlüğü de gözlenerek en
uygun üretim yüzeyi karşılaştırmalı olarak incelenmiştir.  Sonuç olarak agar ve poliüretan destek
malzemelerinin, kum sertleşmesinde destek malzemesi açısından daha etkili
olduğu bulunmuştur. Artan kum kalınlığının sertliği azalttığı ve 50 mM CaCl₂
konsantrasyonunun optimum miktar olduğu belirlenmiştir. Bu çalışma yapı sektörü
ve çimento sektörü açısından yenilikçi, çevre dostu ve biyoteknolojik bir
yöntemin optimizasyonu niteliğindedir.

Keywords

Biyo-kalsifikasyon, Biyo-çimento, Agar, Poliüretan, Filtre kâğıdı

Project Number

2209A

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