Research Article

Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation

Volume: 6 Number: 2 December 31, 2025
EN TR

Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation

Abstract

Mikroorganizmalar, toprak ekosisteminin işlevi, sağlığı, verimliliği, üretkenliği, kalitesi ve sürdürülebilir gelişimi için gereklidir. Bu çalışma, siyah kimyonun yetiştirildiği toprakların biyokimyasal ve mikrobiyal özellikleri üzerinde bakteri formülasyonlarının etkilerini belirlemek için yürütülmüştür. Altı tek suş (Pseudomonas fluorescens RC24, P. fluorescens RC36, P. fluorescens RC38, Bacillus licheniformis RC33, B. megaterium RC61 ve B. subtilis RC121), iki adet çift kombinasyon (Pseudomonas fluorescens RC24 + Bacillus megaterium RC61 ve Pseudomonas fluorescens RC36 + Bacillus subtilis RC121) ve üç adet üçlü kombinasyondaki (Pseudomonas fluorescens RC24 + Bacillus megaterium RC61 + Bacillus suptilis RC121, Pseudomonas fluorescens RC36 + Bacillus licheniformis RC33 + Bacillus megaterium RC61 ve Pseudomonas fluorescens RC38 + Bacillus licheniformis RC33 + Bacillus subtilis RC121) formülasyonlarının üreaz, katalaz, dehidrogenaz ve alkali fosfataz enzim aktiviteleri, ekstrakte edilebilir amonyum ve nitrat azot içerikleri, toprak solunumu ve mezofil aerobik mikroorganizma sayıları üzerine olası etkileri araştırıldı. Toprak biyolojik özelliklerinin farklı düzeylerde uygulanan bakteriyel uygulamalardan etkilendiği belirlenmiş, en etkili bakteriyel uygulamalar Pseudomonas fluorescens RC36, Pseudomonas fluorescens RC38, Pseudomonas fluorescens RC24 olarak sıralanmış ve hepsinin araştırılan toprak biyolojik özelliklerini olumlu yönde etkilediği görülmüştür. Tekli bakteriyel uygulamaların ikili ve üçlü bakteriyel kombinasyonlardan daha etkili olduğu sonucuna varılmıştır.

Keywords

Supporting Institution

This study has not been published or presented in any form previously. The research was carried out on the agricultural fields of the Balıkesir Metropolitan Municipality Farmer Training Center (BAÇEM). We extend our sincere thanks to the institution for their collaboration.

Ethical Statement

There is no need for any ethics committee decision for the work. The authors of the article declare that the data, information and documents presented in this article were obtained within the framework of academic and ethical rules, and that all information, documents, evaluations and results were presented in accordance with scientific ethics and moral rules.

Thanks

This study has not been published or presented in any form previously. The research was carried out on the agricultural fields of the Balıkesir Metropolitan Municipality Farmer Training Center (BAÇEM). We extend our sincere thanks to the institution for their collaboration.

References

  1. Ahmed, T., Shahid, M., Noman, M., Hussain, S., Khan, M. A., Zubair, M., ... Mahmood, F. (2019). Plant growth-promoting rhizobacteria as biological tools for nutrient management and soil sustainability. In Plant Growth Promoting Rhizobacteria for Agricultural Sustainability (pp. 95-110). Springer, Singapore.
  2. Akçura, S., Çakmakçı, R. (2023). Bitki gelişimini teşvik edici bakterilerin şam çörek otunda (Nigella damascena L.) bazı bitkisel özellikler üzerine etkisi. ISPEC Journal of Agricultural Sciences, 7(3), 472-488 (in Turkish).
  3. Akter, A., Zuan, A. T. K., Kasim, S., Amin, A. M., Ab Aziz, Z. F., Md Rahmatullah, N., Sadeq, B. M., Chompa, S. S., Rahman, Md E. (2023). Nitrogen Dynamics in Soil Treated with Plant-growth Promoting Bacteria and Urea Fertilizer. Pertanika Journal of Science & Technology, 31(6), 2995-3011. https://doi.org/10.47836/pjst.31.6.20.
  4. Allison, L. E., & Moodie, C. D. (1965). Carbonate. In: C.A. Black et al.(ed.) Methods of Soil Analysis, Part 2. Agronomy, 9, 1379-1400. Am. Soc. Of Agron., Inc., Madison, Wisconsin, USA.
  5. Allmaras, R. R., & Gardner, C. O. (1956). Soil Sampling for Moisture Determination in Irrigation Experiments 1. Agronomy Journal, 48(1), 15-17. e-ISSN: 1435-0645.
  6. Amezquita-Aviles, C. F., Coronel-Acosta, C. B., Santos-Villalobos, S. D. L., Santoyo, G., & Parra-Cota, F. I. (2022). Characterization of native plant growth-promoting bacteria (PGPB) and their effect on the development of maize (Zea mays L.). Biotecnia, 24(1), 15-22. https://doi.org/10.18633/biotecnia.v24i1.1353.
  7. Anderson, J. P. E. (1983). Soil respiration. In: Methods of Soil Analysis Part 2 Chemical and Microbiological Properties (Ed. JPE. Anderson), 831-871. http://doi.org/10.2134/agronmonogr9.2.2ed.c41.
  8. Anderson, T. H., & Domsch, K. H. (2010). Soil microbial biomass: the eco-physiological approach. Soil Biology and Biochemistry, 42(12), 2039-2043. http://doi.org/10.1016/j.soilbio.2010.06.026.

Details

Primary Language

English

Subjects

Enzyme and Microbial Biotechnology in Agriculture

Journal Section

Research Article

Publication Date

December 31, 2025

Submission Date

May 29, 2025

Acceptance Date

December 21, 2025

Published in Issue

Year 2025 Volume: 6 Number: 2

APA
Türkmen, C., Akçura, S., Çikili, Y., Cakmakcı, R., & Müftüoğlu, N. M. (2025). Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ, 6(2), 147-157. https://izlik.org/JA85BW89WA
AMA
1.Türkmen C, Akçura S, Çikili Y, Cakmakcı R, Müftüoğlu NM. Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ. 2025;6(2):147-157. https://izlik.org/JA85BW89WA
Chicago
Türkmen, Cafer, Sevim Akçura, Yakup Çikili, Ramazan Cakmakcı, and Nuray Mücellâ Müftüoğlu. 2025. “Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation”. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ 6 (2): 147-57. https://izlik.org/JA85BW89WA.
EndNote
Türkmen C, Akçura S, Çikili Y, Cakmakcı R, Müftüoğlu NM (December 1, 2025) Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ 6 2 147–157.
IEEE
[1]C. Türkmen, S. Akçura, Y. Çikili, R. Cakmakcı, and N. M. Müftüoğlu, “Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation”, BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ, vol. 6, no. 2, pp. 147–157, Dec. 2025, [Online]. Available: https://izlik.org/JA85BW89WA
ISNAD
Türkmen, Cafer - Akçura, Sevim - Çikili, Yakup - Cakmakcı, Ramazan - Müftüoğlu, Nuray Mücellâ. “Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation”. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ 6/2 (December 1, 2025): 147-157. https://izlik.org/JA85BW89WA.
JAMA
1.Türkmen C, Akçura S, Çikili Y, Cakmakcı R, Müftüoğlu NM. Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ. 2025;6:147–157.
MLA
Türkmen, Cafer, et al. “Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation”. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ, vol. 6, no. 2, Dec. 2025, pp. 147-5, https://izlik.org/JA85BW89WA.
Vancouver
1.Cafer Türkmen, Sevim Akçura, Yakup Çikili, Ramazan Cakmakcı, Nuray Mücellâ Müftüoğlu. Effects of Single, Double and Triple Bacterial Formulations on Soil Biological Properties in Black Cumin Cultivation. BİLİM-TEKNOLOJİ-YENİLİK EKOSİSTEMİ DERGİSİ [Internet]. 2025 Dec. 1;6(2):147-5. Available from: https://izlik.org/JA85BW89WA