MTT-Based Assessment of the Anticancer Potential of a Known 1,2-Disubstituted Benzimidazole Compound on DLD-1 Cell Line
Abstract
In this study, the cytotoxic effect of 1-(4-methylbenzyl)-2-p-tolyl-1H-benzo[d]imidazole (SL-13) was investigated on human colorectal cancer DLD-1 cells using the MTT assay. Treatment with SL-13 at various concentrations (100, 50, and 25 µM) for 72 hours resulted in a potent cytotoxic response with an IC₅₀ value of 6.093 µM, indicating strong antiproliferative activity. This potency surpasses that reported for many other benzimidazole derivatives tested against DLD-1 cells. These findings show that SL-13 is a promising candidate for further development in colorectal cancer therapy, warranting additional mechanistic and in vivo studies to elucidate its therapeutic potential.
Keywords
Benzimidazole, Colorectal Cancer, Cytotoxic Activity, DLD-1
Supporting Institution
Süleyman Demirel University
Project Number
TSG-2024-9516
Ethical Statement
As this research was conducted entirely on established cancer cell lines, ethics committee approval was not required.
Thanks
SA would like to thank the Suleyman Demirel University Research Fund for financial support with project number TSG-2024-9516.
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